OPTIMIZATION OF LIPID PRODUCTION BY OLEAGINOUS YEAST USING RESPONSE SURFACE METHODOLOGY
Keywords:
Rhodotorulagraminis, response surface methodology, ipid, oleaginous yeastAbstract
The effects of growth parameters of Rhodotorula graminis TISTR 5124 in batch fermentation were studied and optimized for lipid production by using response surface methodology via a Box-Behnken Design. Values of the fermentation parameters affecting the lipid production were varied as follows: carbon sources (glucose, glucose and glycerol and glycerol), temperatures (28, 30, and 32oC) and shaking speeds (150, 200, 250 rpm). Fermentation was carried out in 100 mL Erlenmeyer flasks with a 24 h cultivation time. After eliminating insignificant terms, we found that a good fit (R2 = 0.7555) for lipid production was given by the quadratic regression relationship:Lipid = (4.00*Temperature)- (4.75*Carbon) + (0.70*Shaking speed) – (0.02*Temperature*Shaking speed) -116.0. The results showed that lipid production was significantly influenced by carbon source (p < 0.0001). For the range of conditions studied, we found that the highest yield of lipid was 17.40 g/L, which was obtained using glycerol as sole carbon source at 10 g/L, a temperature of 28oC and a shaking speed of 239 rpm. We found that a good fit (R2 = 0.9107) for the biomass production results was given by the quadratic regression relationship: Biomass = 64.29 -(41.13*Carbon) – (0.19*Temperature) – (0.48*Shaking speed) + (1.38*Carbon* Temperature) – (0.03* Carbon*Shake speed) + (1.28E - 003* Shake speed 2). The highest biomass concentration produced was 27.99 g/L, which was obtained using glycerol as sole carbon source, a temperature of 28oC and a shaking speed of 239 rpm. The mathematical relationship of lipid production can be scaled up to apply to fermentation in a laboratory scale bioreactor.
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