Effect of astaxanthin and yeast (Saccharomyces cerevisiae) supplemented in diets on  growth performance and resistant against virulence Vibrio parahaemolyticus (Vp AHPND) in sand worms (Perinereis nuntia)

Chompunut  Samhuay; Orapint Jintasataporn; Ruangvit  Yoonpundh

Authors

Chompunut Samhuay Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand
Orapint Jintasataporn Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand
Ruangvit Yoonpundh Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand

Keywords:

astaxanthin, Perinereis nuntia, sand worm, yeast, growth performance, Vibrio parahaemolyticus(Vp AHPND)

Abstract

Sand worm (Perinereis nuntia) are commonly used to feed shrimp broodstocks in Thailand. The wild sandworm is in serious trouble, because of this the production from culture in a biosecurity system hatchery is necessary. The objective of this research was to study the effect of feeding supplementation with astaxanthin and yeast (Saccharomyces cerevisiae.) on growth, chemical composition and inhibitory effect against the growth of Vibrio parahaemolyticus (Acute Hepatopancreatic Necrosis Disease; AHPND) of farmed sand worms (Perinereis nuntia).
A completely randomized design (CRD) was carried out using 4 treatments with 3 replications. The sand worms (average weight of 0.05 g) were reared in black plastic tanks and fed for 90 days with 4 different types of diet as follows: T1, commercial Pacific white shrimp diet (control); T2, commercial Pacific white shrimp diet supplementation with astaxanthin (AS); T3, commercial Pacific white shrimp diet supplementation with live cell yeast (LY); and T4, commercial Pacific white shrimp diet supplementation with autolyzed yeast (AY). For the results, the sand worms fed with AS, LY and AY diets (T2,T3,T4) had weight gain, average daily gain, specific growth rate and survival rate significantly higher than the control group (p<0.05). Moreover, feed intake and feed conversion ratio of sand worms in T2, T3 and T4 were significantly lower than the control group (p<0.05). Protein and lipid contents of the sand worms meat in T2, T3 and T4 were significantly higher than the control group (p<0.05). The results indicated that feed supplementation with astaxanthin and yeast (both live cell and autolyzed yeast) increased growth, protein and lipids contents of farmed sand worms. The coelomic fluid of sandworms in T2, T3 and T4 showed significantly higher inhibitory effect against the growth of Vp _AHPND than the control group. The results indicated that feed supplementation with astaxanthin and yeast increased growth, protein, lipids contents and inhibitory effect against the growth of Vp AHPND. These findings could significantly contribute to the value of farmed sand worm in further studies.

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