Effect of astaxanthin and yeast (Saccharomyces cerevisiae) supplemented in diets on growth performance and resistant against virulence Vibrio parahaemolyticus (Vp AHPND) in sand worms (Perinereis nuntia)
Keywords:astaxanthin, Perinereis nuntia, sand worm, yeast, growth performance, Vibrio parahaemolyticus(Vp AHPND)
Sand worm (Perinereis nuntia) are commonly used to feed shrimp broodstocks in Thailand. The wild sandworm is in serious trouble, because of this the production from culture in a biosecurity system hatchery is necessary. The objective of this research was to study the effect of feeding supplementation with astaxanthin and yeast (Saccharomyces cerevisiae.) on growth, chemical composition and inhibitory effect against the growth of Vibrio parahaemolyticus (Acute Hepatopancreatic Necrosis Disease; AHPND) of farmed sand worms (Perinereis nuntia).
A completely randomized design (CRD) was carried out using 4 treatments with 3 replications. The sand worms (average weight of 0.05 g) were reared in black plastic tanks and fed for 90 days with 4 different types of diet as follows: T1, commercial Pacific white shrimp diet (control); T2, commercial Pacific white shrimp diet supplementation with astaxanthin (AS); T3, commercial Pacific white shrimp diet supplementation with live cell yeast (LY); and T4, commercial Pacific white shrimp diet supplementation with autolyzed yeast (AY). For the results, the sand worms fed with AS, LY and AY diets (T2,T3,T4) had weight gain, average daily gain, specific growth rate and survival rate significantly higher than the control group (p<0.05). Moreover, feed intake and feed conversion ratio of sand worms in T2, T3 and T4 were significantly lower than the control group (p<0.05). Protein and lipid contents of the sand worms meat in T2, T3 and T4 were significantly higher than the control group (p<0.05). The results indicated that feed supplementation with astaxanthin and yeast (both live cell and autolyzed yeast) increased growth, protein and lipids contents of farmed sand worms. The coelomic fluid of sandworms in T2, T3 and T4 showed significantly higher inhibitory effect against the growth of Vp AHPND than the control group. The results indicated that feed supplementation with astaxanthin and yeast increased growth, protein, lipids contents and inhibitory effect against the growth of Vp AHPND. These findings could significantly contribute to the value of farmed sand worm in further studies.
AOAC. (2016). Official methods of analysis of the Association of Official Analytical Chemists, 20th ed. Association of Official Analytical Chemists, Gaithersburg, USA. ISBN 0-935584-87-0
Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of pharmaceutical analysis, 6(2), 71-79. DOI: 10.1016/j.jpha.2015.11.005
Bauernfeind, J. C. (1981). Carotenoids as colorants and vitamin A precursors; technological and nutritional applications. ISBN 9780323139779
Bendich, A. (1989). Carotenoids and the immune response. The Journal of nutrition, 119(1), 112-115. DOI:10.1093/jn/119.1.112
Buyuksirit, T., & Kuleasan, H. (2014). Antimicrobial agents produced by yeasts. International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering, 8(10), 1096-1099. DOI: 10.5281/zenodo.1096429
Chanchaichaovivat, A. (2015). Yeast and yeast technology. 1st ed. Bangkok, Thailand: kaothai-advertising-printing. ISBN: 978-974-373-596-7
Chuchird, N. (1995). Effects of astaxanthin on growth survival rate and tolerance to pathogenic bacteria of black tiger shrimp in low salinity conditions. (Master’s thesis, KasetsartUniversity, Thailand). https://kukr.lib.ku.ac.th/db/index.php?/BKN/search_detail/result/214819
Department of Fishery. (2008). Method of water analysis for coastal aquaculture. Bangkok Thailand: Department of Fishery. http://ebook.lib.ku.ac.th/item/2/20160034
Foss, P., Storebakken, T., Schiedt, K., Liaaen-Jensen, S., Austreng, E., & Streiff, K. (1984). Carotenoids in diets for salmonids: I. Pigmentation of rainbow trout with the individual optical isomers of astaxanthin in comparison with canthaxanthin. Aquaculture, 41(3), 213-226. DOI: 10.1016/0044-8486(84)90284-9
Goodwin, T. W. (1980). Biogeochemistry of Carotenoids. In The biochemistry of the carotenoids (pp. 346-349). Springer, Dordrecht. DOI: 10.1007/978-94-009-5860-9_11
Lim, K. C., Yusoff, F. Md., Shariff, M., & Kamarudin, M. S. (2017). Astaxanthin as feed supplement in aquatic animals. Reviews in Aquaculture, 10(3), 738-773. DOI: https://doi.org/10.1111/raq.12200
Lipke, P. N., & Ovalle, R. (1998). Cell wall architecture in yeast: new structure and new challenges. Journal of bacteriology, 180(15), 3735-3740. DOI: 10.1128/jb.180.15.3735-3740.1998
Paibulkichakul, C. (2002). Effects of dietary astaxanthin and fish oil on maturation of pond-reared black tiger prawn Penaeus monodon (Doctoral dissertation, Chulalongkorn University, Thailand).
Rajabi, B., Salarzadeh, A. R., Yahyavi, M., Masandani, S., & Niromand, M. (2012). Effect of astaxanthin pigment on growth performance, survival and pigmentation in postlarval stage of white leg shrimp, Litopenaeus vannamei. Iranian Fisheries Science Research Journal (ISFJ), 21(1), 89-100. DOI: 10.22092/ISFJ.2017.110041
Rumsey, G. L., Hughes, S. G., & Kinsella, J. L. (1990). Use of dietary yeast Saccharomyces cerevisiae nitrogen by lake trout. Journal of the World Aquaculture Society, 21(3), 205-209. DOI:10.1111/j.17497345.
Salarzadeh, A., & Rajabi, B. (2015). The effects of dietary supplementation synthetic Astaxanthin on body astaxanthin, survival, growth of white leg shrimp (Litopenaeus vannamei). International Journal, 3(3), 797-803.
Srimarksuk, A., Chuchird, N., Limsuwan, C., & Purivirojkul, W. (2011). Effects of Yeast Cell Debris on Growth, Survival and Disease Resistance of Pacific White Shrimp Litopenaeus vannamei. Journal of Fisheries and Environment, 35(3), 22-29. DOI:https://li01.tci-thaijo.org/index.php/JFE/
Techaprempreecha, S. (2007). Purification and characterization of antimicrobial peptides from the sandworm Perinereis nuntia savigny (Doctoral dissertation, Chulalongkorn University).http://cuir.car.chula.ac.th
Techaprempreecha, S., Khongchareonporn, N., Chaicharoenpong, C., Aranyakananda, P., Chunhabundit, S., & Petsom, A. (2011). Nutritional composition of farmed and wild sandworms, Perinereis nuntia. Animal feed science and technology, 169(3-4), 265-269.DOI:10.1016/j.anifeedsci.2011.06.007
Xiong, J., Jin, M., Yuan, Y., Luo, J. X., Lu, Y., Zhou, Q. C., & Tan, Z. L. (2018). Dietary nucleotide‐rich yeast supplementation improves growth, innate immunity and intestinal morphology of Pacific white shrimp (Litopenaeus vannamei). Aquaculture nutrition, 24(5), 1425-1435. DOI: 10.1111/anu.1267
How to Cite
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.