Innovation for plant-based foods: Allergen-free vegan meat and egg products from rice processing by-products


  • Yupakanit Puangwerakul Faculty of Food Technology, College of Agricultural and Food, Rangsit University, Patumthani 12000, Thailand
  • Suvimol Soithongsuk Faculty of Food Technology, College of Agricultural and Food, Rangsit University, Patumthani 12000, Thailand


allergy free, bioactive compound, nutritional value, plant-based food, rice protein hydrolysate, vegan egg, vegan meat


This study aimed to develop plant-based vegan meat and egg products that still contain high nutritional and functional components from the by-products of rice processing. The ingredients such as rice malt, red rice malt, and bio-cellulose from rice kombucha processing were prepared and mixed to produce vegan meat and egg products. The optimal ratio of a rice protein hydrolysate, as an alternative emulsifier to commercial food hydrocolloid, was also investigated.  The results showed that the vegan meat contained comparable content of protein and minerals, with 16 times lower fat and 3 times higher dietary fiber than the commercial vegan meat. Similarly, the vegan egg product contained the same content of protein and minerals, but with 14 times lower fat and 4 times higher fiber than the product from a real egg. The vegan meat and egg products contained higher polyphenol, antioxidant, ACE inhibitory peptide, D-saccharic acid 1,4-lactone (DSL), and gamma-aminobutyric acid (GABA) than the real meat, real egg, and commercial vegan products. The commercial hydrocolloid can be substituted by the natural rice protein hydrolysate at 70% and 100% for vegan meat and egg products, respectively. Both products exhibited comparable properties to the commercial products in terms of color, firmness, oil holding capacity, size expansion, as well as sensory attributes (color and taste). The shelf-life study indicated that the vegan meat and vegan egg powder could be stored at -18°C and at ambient temperature for 18 and 16 months, respectively. Product cost and pricing calculations showed that the products were highly competitive. This innovative research presented a high potential for further commercialization for allergen-free plant-based proteins.


Allen, M. (2019). New study highlights plant-based and cultivated meat acceptance in the U.S., China, and India. Retrieved from

Ang, J. F. (1991). Water retention capacity and viscosity effect of powdered cellulose. Journal of Food Science, 56(6), 1682-1684.

Association of Official Agricultural Chemists (AOAC), & Horwitz, W. (1975). Official methods of analysis (Vol. 222). Association of Official Analytical Chemists: Washington, DC.

Bhatia, M. (2019). Food habits of Indians:Ipsos study 74% Indians are content with their current body weight; 63% Indians are willing to eat a plant based substitute for meat The study busts myths about Indians. Retrieved from

Bogracheva, T. Y., Morris, V. J., Ring, S. G., & Hedley, C. L. (1998). The granular structure of C‐type pea starch and its role in gelatinization. Biopolymers: Original Research on Biomolecules, 45(4), 323-332.

Brand-Williams, W., Cuvelier, M. E., & Berset, C. L. W. T. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food science and Technology, 28(1), 25-30.

Chamba, M. V. M., Hua, Y., & Simwaka, J. E. (2014). Comparative sensory evaluation of soy protein isolates extracted from full-fat and defatted flours using natural and conventional synthetic extraction chemicals. International Food Research Journal, 21(1). 209-215.

Chassagnez-Mendez, A. L., Correa, N. C. F., Franca, L. F., Machado, N. T., & Araujo, M. E. (2000). A mass transfer model applied to the supercritical extraction with CO2 of curcumins from turmeric rhizomes (Curcuma longa L). Brazilian Journal of Chemical Engineering, 17(3), 315-322.

Choudhury, D., Singh, S., Seah, J. S. H., Yeo, D. C. L., & Tan, L. P. (2020). Commercialization of plant-based meat alternatives. Trends in Plant science, 25(11), 1055-1058.

Danziger, L. (2020). Survey: 23% of Americans eating more plant-based foods during COVID-19. Retrieved from

Dijksterhuis, J., & Samson, R. A. (2007). Food Mycology: A multifaceted approach to fungi and food. Taylor & Francis: Florida, USA.

Franck, T. (2019). Alternative meat to become $140 billion industry in a decade, Barclays predicts. Retrieved from

Galliard, T. & Bowler, P. (1987). Morphology and composition of starch. In T. Gailliard (Ed.).Starch: Properties and Potential. John Wiley and Sons: New York.

Geijer, T. (2017). Executive summary: the protein shift in Europe. Retrieved from

Grossi, G., Goglio, P., Vitali, A., & Williams, A. (2019). Livestock and climate change: impact of livestock on climate and mitigation strategies. Anim. Front, 9(1),69–76.

Kitaoka, S., & Nakano,Y. (1969). Colorimetric determination of -amino acids. The Journal of Biochemistry, 66(1), 87-94.

Kreutz, J. M., Adriaanse, M. P., van der Ploeg, E. M., & Vreugdenhil, A. C. (2020). Narrative review: nutrient deficiencies in adults and children with treated and untreated celiac disease. Nutrients, 12(2), 500. DOI:10.3390/nu12020500

Kunze, W. (2004). Technology brewing and malting (3rd international edition ed.).Berlin: VBL berlin.

Li, D. (2014). Effect of the vegetarian diet on non‐communicable diseases. Journal of the Science of Food and Agriculture, 94(2), 169-173. DOI:10.1002/jsfa.6362.Epub2013Oct2.

Li, G. H., Liu, H., Shi, Y. H., & Le, G. W. (2005). Direct spectrophotometric measurement of angiotensin I-converting enzyme inhibitory activity for screening bioactive peptides. Journal of pharmaceutical and biomedical analysis, 37(2), 219-224.

Liang, Y., & Kristinsson, H. G. (2005). Influence of pH‐Induced unfolding and refolding of egg albumen on its foaming properties. Journal of food science, 70(3), C222-C230.

Lin, M. N., Chiu, T. H., & Chang, C. E. (2019). The impact of plant-based dietary pattern on dementia risk: a prospective cohort study. Innovation in Aging, 3(Suppl1), S734. DOI:10.1093/geroni/igz038.2691

Maturin, L & Peeler, J. T. (2001). BAM Chapter 3: Aerobic Plate Count. Retrieved from

Mintel Press Team. (2018). Thai consumers are on the road to self-betterment. Retrieved from

Nuntramas, P., Suprasert, A., & Akegachan, P. (2020). Plant-based food. Retrieved from Krungthai COMPASS. Retrieved from EconomyResourcesDownload_452Plant_based_Food.pdf

Ottaway, P. B. (1993). Stability of vitamins in food. In The technology of vitamins in food (pp. 90-113). Boston, MA, USA: Springer.

Packer, L., Hiramatsu, M., & Yoshikawa, T. (Eds.). (1999). Antioxidant food supplements in human health. Academic Press: USA.

Prag, A. A., & Henriksen, C. B. (2020). Transition from animal-based to plant-based food production to reduce greenhouse gas emissions from agriculture—the case of Denmark. Sustainability, 12(19), 8228.

Puangwerakul, Y. and Soithongsuk,S. (2021). Research and development of plant-based entirely nonallergenic egg and business plan. Research Report of Academic Service Center (ASC), Rangsit University: Patumthani, Thailand.

Puangwerakul, Y., & Soithongsuk, S. (2020). Research and development of vegan faux-meat fortified with rice protein hydrolysate and business plan. Research Report of Ministry of Higher Education, Science, Research and Innovation (MHESI): Bangkok, Thailand.

Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American journal of Enology and Viticulture, 16(3), 144-158.

Steele, R. (Ed.). (2004). Understanding and measuring the shelf-life of food.

Washington, DC, USA: Woodhead Publishing.

Steinfeld, H., Gerber, P., Wassenaar, T. D., Castel, V., Rosales, M., Rosales, M., & de Haan, C. (2006). Livestock's long shadow: environmental issues and options. Food & Agriculture Org: Rome, Italy.

Swinkels, J. J. M. (1985). Composition and properties of commercial native starches. Starch‐Stärke, 37(1), 1-5.

Wang, K., Gan, X., Tang, X., Wang, S., & Tan, H. (2010). Determination of d-saccharic acid-1, 4-lactone from brewed kombucha broth by high-performance capillary electrophoresis. Journal of Chromatography B, 878(3-4), 371-374.

Wrolstad, R. E., Acree, T. E., Decker, E. A., Penner, M. H., Reid, D. S., Schwartz, S. J., ... & Sporns, P. (Eds.). (2005). Handbook of food analytical chemistry, volume 1: Water, proteins, enzymes, lipids, and carbohydrates. John Wiley & Sons.

Xu, X., Sharma, P., Shu, S., Lin, T. S., Ciais, P., Tubiello, F. N., ... & Jain, A. K. (2021). Global greenhouse gas emissions from animal-based foods are twice those of plant-based foods. Nature Food, 2(9), 724-732.




How to Cite

Puangwerakul, Y. ., & Soithongsuk, S. . (2023). Innovation for plant-based foods: Allergen-free vegan meat and egg products from rice processing by-products . Journal of Current Science and Technology, 12(2), 358–371. Retrieved from



Research Article