COMPARATIVE EVALUATION OF IFN-γ SERUM LEVELS AND mRNA EXPRESSION IN CONNECTION WITH GALLSTONE PATIENTS: A CASE-CONTROLLED STUDY
DOI:
https://doi.org/10.55766/sujst9608Keywords:
Diagnostic Marker, Gallstone disease, Inflammatory Cytokine, mRNA expressionAbstract
IFN-γ is a complex cytokine that plays a major role in immune system regulation. In addition to strengthening host defence, IFN-γ also causes autoantibodies, activates autologous cytotoxic T cells, and kills target cells, all of which contribute to a chain of several diseases. The current study enrolled 30 gallstone patients and 30 healthy controls. Blood and serum concentrations were considered for quantifying relative mRNA expression levels, followed by evaluation of serum concentration for the IFN-γ gene by comparing it with healthy controls. The current study has shown significant expression of mRNA levels for the IFN-γ gene with a 3.4008 fold change difference, having a P<0.008. At the same time, serum concentrations of gallstone patients found high levels of IFN-γ (17.06 vs 33.12) when compared to Healthy controls with a (P<0.0001). Along with these safety parameters like ALP (P<0.004), Bilirubin (P<0.00001), Urea (P<0.0010) serum creatinine (P<0.00001), cholesterol, HDL and VLDL (P<0.0005, P<0.0002 and P< 0.00001) and Hematological tests like RBC, Lymphocytes and ESR (P<0.0035, P<0.0046, P<0.00001 and P<0.00006) shown significant increase in patients having gallstone disease when compared with healthy controls. This study has shown a significant mRNA expression and serum levels of IFN-γ in patients when compared to healthy controls. This findings may be a risk aspect for the development of gallstone disease, signifying that it might be utilised as a marker to measure disease activity and pave the way for future disease treatment strategies.
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