OPTIMUM FACTORS FOR PATHENOCARPIC CUCUMBER OVARY CULTURE
DOI:
https://doi.org/10.55766/sujst6874Keywords:
Cucumber (Cucumis sativus L.); Parthenocarpic; Ovary culture; Doubled haploidAbstract
Parthenocarpic cucumbers, which can bear fruit without pollination, produce seedless fruits that are highly desired by consumers, agricultural producers, and breeders. However, the seedless nature of these cucumbers poses challenges for breeding improvements, often requiring extended periods. Ovary culture or unpollinated ovule culture, a plant tissue culture technique, can expedite the production of inbred lines. This research aims to investigate the factors influencing ovules and ovaries culture in parthenocarpic cucumbers by examining the influence of three factors: genotype (Hom Bai Toey, Smile, Basimah and Cengel), incubation temperature (25°C and 33°C), and TDZ concentration (0.00, 0.04, 0.06, and 0.08 mg/L). The results showed that Smile with 0.06 mg/L TDZ induced the highest percentage of ELS at 90.0%, which was not significantly different from Smile with 0.08 mg/L TDZ and Cengel with 0.08 mg/L TDZ (83.3% and 70.0%, respectively). These cultures were then transferred to Murashige and Skoog (MS) medium without hormones to induce callus or ELS development into plants, resulting in the induction of five plants. Flow cytometer and ISSR marker analyses confirmed that 40% of the plants were double haploids and 60% were mixoploids. Additionally, ISSR analysis revealed that the double haploid plants derived from cucumber ovary culture exhibited genetic differences from all the mother plants.
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