EVALUATION OF ANTICANCER POTENTIAL OF EPIPREMNUM AUREUM: AN IN-VITRO AND IN-SILICO APPROACH
Anticancer potential of Epipremnum aureum
DOI:
https://doi.org/10.55766/sujst5419Keywords:
Anticancer, Epipremnum aureum, Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl) ethyl ester, Octadecanoic acid, 2, 3-dihydroxypropyl ester, Yes-Associated ProteinAbstract
The use of phytochemicals in cancer therapeutics is an approach that has gained significant attention in recent years. Several plants commonly found in the environment have been observed to possess secondary metabolites with medicinal properties. This study aims to examine the anticancer activity of the methanolic extract of Epipremnum aureum (MEEA), a well-known ornamental vine. The constituents of the extract have been identified by the use of biochemical tests, as well as Gas Chromatography Mass Spectrometry. The extract has been shown to exhibit a cytotoxic effect against the HCT-116 cell line, with an IC50 value of 35.7 μg/mL. Docking and Molecular Dynamics Simulation (MDS) conducted against the Yes-associated protein (YAP) using the constituents of E. aureum as ligands showed that Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester (HE) and Octadecanoic acid, 2,3-dihydroxypropyl ester (OE) may be able to inhibit the interaction of YAP with TEA DNA-binding domain protein (TEAD) of the Hippo pathway, and hence prevent cell proliferation.
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