ABUNDANCE OF POLLUTION-INDICATOR BACTERIA IN CIMANUK WATERS, INDRAMAYU

Pollution-Indicator Bacteria in Cimanuk Waters

Authors

  • Nur Fitriah Afianti National Research and Innovation Agency
  • Lies Indah Sutiknowati National Research and Innovation Agency
  • Risky Ayu Kristanti National Research and Innovation Agency

DOI:

https://doi.org/10.55766/sujst-2024-06-e04798

Keywords:

Cimanuk Waters, Bacterial Density, Microbiological Parameter, Pollutant Indicator

Abstract

Cimanuk waters are one of Indonesia’s areas with abundant natural resource potential that have been threatened with contamination due to impulsive population growth and high human activities. Microbiological aspects are one of the parameters that can indicate environmental pollution. Therefore, pollution-indicator bacteria such as coliform and pathogenic bacteria have become one of the most important indicators of environmental conditions. This study conducted an analysis of pollution indicator bacteria at several sampling sites around the Cimanuk estuary to assess the environmental status. Surface seawater (±30 cm) was taken using a Zobell water sampler with
a sterile 300 ml bottle. Samples were subjected to microbiological analysis, including total coliform contaminating bacteria, Escherichia coli, heterotrophic bacteria, halotolerant, and pathogenic bacteria. Results showed severe fecal contamination of stations compared to the WHO standards, and total coliforms varied between stations in the range of 1500-3000 CFU/100mL. The presence of pathogenic bacteria such as E. coli, Vibrio cholera, Aeromonas spp., Salmonela spp., and other bacteria indicative of fecal contamination were reported. Coliform bacteria and E. coli were found at the highest density in coastal waters near the mainland, indicating waste input due to anthropogenic activities. This study shows that Cimanuk waters are not good for biota and recreational studies.

References

Afianti, N.F. and Sutiknowati, L. (2020). Environmental pollution conditions based on microbiological parameters around the mouth of the Cimandiri River. Pelabuhan Ratu Bay, West Java. Biologi Biosfera, 37(3):135-140.

Antonara, S. and Ardura, M.I. (2018). Citrobacter species. In S.S. Long, M. Fischer, and C.G. Prober (Eds.), Principles and practice of pediatric infectious disease, pp. 827-892. Elsevier. https://doi.org/10.1016/C2013-0-19020-4

Barrow, G.I., and Miller, D.C. (1976). Vibrio parahaemolyticus and seafood. Society for Applied Bacteriology Symposium Series, 4:181-195.

Bisimwa, A.M., Kisuya, B., Kazadi, Z.M., Muhaya, B.B., and Kanjonda, A.B. (2022). Monitoring faecal contamination and relationship of physicochemical variables with faecal indicator bacteria number in Bukavu surface water. tributaries of Lake Kivu in Democratic Republic of Congo. Hygiene and Environmental Health Advance, 3:100012. https://doi.org/10.1016/j.heha.2022.100012

Curutiu, C., Iordache, F., Gurban, P., Lazar, V., and Chifiriuc, M.C. (2019). Main microbiological pollutants of bottled waters and beverages. In A.M. Grumezescu and A.M. Holban (Eds.), Bottled and packaged beverages, 1st ed., pp. 403-422. Woodhead Publishing. https://doi.org/10.1016/B978-0-12-815272-0.00014-3

Dziubańska-Kusibab, P.J., Berger, H., Battistini, F., Bouwman, B.A.M., Iftekhar, A., Katainen, R., Crosetto, N., Orozco, M., Aaltonen, L.A., and Meyer, T.F. (2020). Colibactin DNA damage signature indicates causative role in colorectal cancer. Nature Medicine, 26:1,063-1,069. https://doi.org/10.1038/s41591-020-0908-2

Flynn, A., Davis, J.K., Atherly, E., Olson, G., Bower, J.C., DePaola, A., and Curriero, F.C. (2019). Associations of environmental conditions and Vibrio parahaemolyticus genetic markers in Washington State Pacific oysters. Frontiers in Microbiology, 10:2797. https://doi.org/10.3389/fmicb.2019.02797

Follett, C.L., Dutkiewicz, S., Ribalet, F., Zakem, E., Caron, D., Armbrust, E.V., and Follows, M.J. (2022). Trophic interactions with heterotrophic bacteria limit the range of Prochlorococcus. Proceedings of the National Academy of Sciences, 119(2):e2110993118. https://doi.org/10.1073/pnas.2110993118

Jung, S.-W. (2018). A foodborne outbreak of gastroenteritis caused by Vibrio parahaemolyticus associated with cross-contamination from squid in Korea. Epidemiology and Health, 40:e201856. https://doi.org/10.4178/epih.e2018056

Khouadja, S., Suffredini, E., Spagnoletti, M., Croci, L., Colombo, M.M., and Amina, B. (2013). Presence of pathogenic Vibrio parahaemolyticus in waters and seafood from the Tunisian Sea. World Journal of Microbiology and Biotechnology, 29:1,341-1,348. https://doi.org/10.1007/s11274-013-1297-1

Kristanti, R.A., Hadibarata, T., Syafrudin, M., Yilmaz, M., and Abdullah, S. (2022). Microbiological contaminants in drinking water: Current status and challenges. Water, Air, and Soil Pollution, 233(8):299. https://doi.org/10.1007/s11270-022-05698-3

Letchumanan, V., Chan, K.-G., and Lee, L.-H. (2014). Vibrio parahaemolyticus: A review on the pathogenesis, prevalence, and advance molecular identification techniques. Frontiers in Microbiology, 5:705. https://doi.org/10.3389/fmicb.2014.00705

Li, D. and Liu, S. (2019). Water quality monitoring in aquaculture. In D. Li and S. Liu (Eds.), Water quality monitoring and management, 1st ed., pp. 303-328. Academic Press. https://doi.org/10.1016/B978-0-12-811330-1.00012-0

Li, F., Xiong, X.-S., Yang, Y.-Y., Wang, J.-J., Wang, M.-M., Tang, J.-W., Liu, Q.-H., Wang, L., and Gu, B. (2021). Effects of NaCl concentrations on growth patterns, phenotypes associated with virulence, and energy metabolism in Escherichia coli BW 25113. Frontiers in Microbiology, 12:705326. https://doi.org/10.3389/fmicb.2021.705326

Ma, Q., Meng, N., Li, Y., and Wang, J. (2021). Occurrence, impacts, and microbial transformation of 3-methylindole (skatole): A critical review. Journal of Hazardous Materials, 416:126181. https://doi.org/10.1016/j.jhazmat.2021.126181

Megantara, I., Sylviana, N., Amira, P.A., Pradini, G.W., Krissanti, I., and Lesmana, R. (2023). Potential of waterbodies as a reservoir of Escherichia coli pathogens and the spread of antibiotic resistance in Indonesia aquatic environment. Water, Sanitation and Hygiene for Development, 13(10):776-792. https://doi.org/10.2166/washdev.2023.040

Mekasha, S., and Linke, D. (2021). Secretion systems in Gram-negative bacterial fish pathogens. Frontiers in Microbiology, 12:782673. https://doi.org/10.3389/fmicb.2021.782673

Ministry of Environment (MOE). (2004). Guidelines for Ministry of Environment Decree No 51/2004 on Standard Quality of Seawater. Retrieved January 5, 2024, from https://wepa-db.net/wp-content/uploads/2023/02/3_Indonesia_Marine-water-standards_from-the-former-WEPA-HP.pdf.

Mulyani, L.S., Mardiani, R., Ardiana, C., and Nurkamilah, S. (2020). Relationship between distribution of phytoplankton with Cimanuk River water quality. IOP Conference Series: Materials Science and Engineering, 1098:052020. https://doi.org/10.1088/1757-899X/1098/5/052020

Murray, P.R., Rosenthal, K.S., and Pfaller, M.A. (2009). Medical microbiology (6th ed.). Mosby Elsevier.

Nair, S., Zhang, Z., Li, H., Zhao, H., Shen, H., Kao, S.-J., Jiao, N., and Zhao, Y. (2022). Inherent tendency of Synechococcus and heterotrophic bacteria for mutualism on long-term coexistence despite environmental interference. Science Advances, 8:eabf4792. https://doi.org/10.1126/sciadv.abf4792

Nascimento, A., Biguino, B., Borges, C., Cereja, R., Cruz, J.P. C., Sousa, F., Dias, J., Brotas, V., Palma, C., and Brito, A.C. (2021). Tidal variability of water quality parameters in a mesotidal estuary (Sado estuary, Portugal). Scientific Reports, 11:23112. https://doi.org/10.1038/s41598-021-02603-6

Passante, E.K., Dechant, L.E., Paradis, C.J., and McLellan, S.L. (2022). Halophilic bacteria in a Lake Michigan drainage basin as potential biological indicators of chloride-impacted freshwater. Science of the Total Environment, 846:157458. https://doi.org/10.1016/j.scitotenv.2022.157458

Rahaman, S.M.B., Sarder, L., Rahaman, M.S., Ghosh, A.K., Biswas, S.K., Siraj, S.M.S., Huq, K.A., Hasanuzzaman, A.F.M., and Islam, S.S. (2013). Nutrient dynamics in the Sundarbans mangrove estuarine system of Bangladesh under different weather and tidal cycles. Ecological Processes, 2:29. https://doi.org/10.1186/2192-1709-2-29

Roseline, A. (2019). Hydrocarbon degradation potential of heterotrophic bacteria in Nigeria. LAP Lambert Academic Publishing.

Safitri, L.F., Widyorini, N., and Jati, O.E. (2018). Analysis of total abundance of coliform bacteria at the Sayung River Estuary, Morosari, Demak. Indonesian Journal of Fisheries Science and Technology, 14(1):30-35. https://doi.org/10.14710/ijfst.14.1.30-35

Shen, C. and Zhang, Y. (2022). Total plant counts and coliform counts of pond water. In Shen, C., and Zhang, Y. (Eds.), Introductory microbiology lab skill and techniques in food science. 1st ed., pp. 143-148. Academic Press. https://doi.org/10.1016/B978-0-12-821678-1.00003-4

Solihuddin, T., Husrin, S., Salim, H.L., Kepel, T.L., Mustikasari, E., Heriati, A., Ati, R.N.A., Purbani, D., Mbay, L.O.N., Indriasari, V.Y., and Berliana, B. (2021). Coastal erosion on the north coast of Java: Adaptation strategies and coastal management. IOP Conference Series: Earth and Environmental Sciences, 777:012035. https://doi.org/10.1088/1755-1315/777/1/012035

Some, S., Mondal, R., Mitra, D., Jain, D., Verma, D., and Das, S. (2021). Microbial pollution of water with special reference to coliform bacteria and their nexus with environment. Energy Nexus, 1:100008. https://doi.org/10.1016/j.nexus.2021.100008

Stec, J., Kosikowska, U., Mendrycka, M., Stępień-Pyśniak, D., Niedźwiedzka-Rystwej, P., Bębnowska, D., Hrynkiewicz, R., Ziętara-Wysocka, J., and Grywalska, E. (2022). Opportunistic pathogens of recreational waters with emphasis on antimicrobial resistance: A possible subject of human health concern. International Journal of Environmental Research and Public Health, 19(12):7308. https://doi.org/10.3390/ijerph19127308

Valente, C.D.V. and Wan, A.H.L. (2021). Vibrio and major commercially important vibriosis diseases in decapod crustaceans. Journal of Invertebrate Pathology, 181:107527. https://doi.org/10.1016/j.jip.2020.107527

Wen, X., Chen, F., Lin, Y., Zhu, H., Yuan, F., Kuang, D., Jia, Z., and Yuan, Z. (2020). Microbial indicators and their use for monitoring drinking water quality: A review. Sustainability, 12(6):2249. https://doi.org/10.3390/su12062249

World Health Organization (WHO). (2021). Guidelines on recreational water quality: Volume 1: Coastal and fresh waters. Geneva: World Health Organization.

Wulandari, I., Yogaswara, D., Khozanah, K., Edward, E., Rositasari, R., and Falahudin, D. (2019). Pengukuran Total Petroleum Hidrokarbon (TPH) Melalui Pendekatan Kadar Minyak-Lemak dalam Sedimen di Perairan Delta Cimanuk, Jawa Barat. OLDI (Oseanologi dan Limnologi di Indonesia), 4(2):123-132.

Zhang, X-H. and Austin, B. (2005). Haemolysins in vibrio species. Journal of Applied Microbiology, 98(5):1,011-1,019. https://doi.org/10.1111/j.1365-2672.2005.02584.x.

Zhao, X., Liu, J., Zhuo, S., Zheng, Y., Wu, Y., Kogure, K., and Zhang, X.-H. (2020). Diversity of culturable heterotrophic bacteria from the Mariana Trench and their ability to degrade macromolecules. Marine Life and Science Technology, 2:181-193. https://doi.org/10.1007/s42995-020-00027-1

Downloads

Published

2025-01-29

How to Cite

Afianti, N. F., Sutiknowati, L. I., & Kristanti, R. A. (2025). ABUNDANCE OF POLLUTION-INDICATOR BACTERIA IN CIMANUK WATERS, INDRAMAYU: Pollution-Indicator Bacteria in Cimanuk Waters. Suranaree Journal of Science and Technology, 31(6), 030235(1–7). https://doi.org/10.55766/sujst-2024-06-e04798

Issue

Section

Research Article

Categories