EMBRYOTOXIC AND TERATOGENIC EFFECTS OF MONOSODIUM GLUTAMATE AND SACCHARIN ON WADER (Rasbora lateristriata) FISH
DOI:
https://doi.org/10.55766/sujst-2024-06-e04047Keywords:
Abnormality, Embryo development, MSG, Saccharin, Teratogenicity, WaderAbstract
The widespread utilization of food additives has garnered increasing attention owing to the safety implications. The continuous reassessment of potential toxicogenic and teratogenic risks associated with food additives is imperative. Monosodium glutamate (MSG) and saccharin, which are prevalently used in the food industry, were the focus of this study. This research aimed to evaluate the effects of MSG, saccharin, and the MSG-saccharin combination on the embryos of wader (Rasbora lateristriata) fish. Observations were conducted at 24, 48, and 72 h postfertilization (hpf) following substance administration. The findings indicated that the MSG- and MSG-saccharin-treated groups exhibited higher incidences of spinal abnormalities, pericardial edema, and bradycardia. Regarding safety, the survival rates of the saccharin-treated groups were higher than those of the MSG- and MSG-saccharin-treated groups. The observations further suggested variations in survival and hatching rates, body pigmentation, and blood clotting. Spinal irregularities, pericardial edema, and bradycardia were the predominant abnormalities recorded. These results establish the developmental risks associated with MSG and its combination with saccharin, emphasizing the need for further investigations to elucidate the mechanisms underlying these abnormalities.
References
Abdelkader, T.S., Seo-Na, C., Tae-Hyun, K., Juha, S., Dongso, K., and Park, J.H. (2012). Teratogenicity and brain aromatase-induction of monosodium glutamate in estrogen-responsive mosaic transgenic zebrafish Danio rerio. African Journal of Biotechnology, 11(48):10,816-10,823. https://doi.org/10.5897/AJB12.1262
Al-Ghamdi, F.A.M. (2022). Pomegranate peel extracts effects to reduce monosodium glutamate toxic effects on chicken embryos: Morphological studies. Saudi Journal of Biological Sciences, 29(2):975-983. https://doi.org/10.1016/j.sjbs.2021.10.004
Azeez, O.H., Alkass, S.Y., and Persike, D.S. (2019). Long-term saccharin consumption and increased risk of obesity, diabetes, hepatic dysfunction, and renal impairment in rats. Medicina, 55(10):681. https://doi.org/10.3390/medicina55100681
Banerjee, A., Mukherjee, S., and Maji, B.K. (2021). Worldwide flavor enhancer monosodium glutamate combined with high lipid diet provokes metabolic alterations and systemic anomalies: An overview. Toxicology Reports, 8:938-961. https://doi.org/10.1016/j.toxrep.2021.04.009
Bayındır Gümüş, A., Keser, A., Tunçer, E., Altuntaş Yıldız, T., and Kepenekci Bayram, İ. (2022). Effect of saccharin, a non-nutritive sweetener, on insulin and blood glucose levels in healthy young men: A crossover trial. Diabetes and Metabolic Syndrome: Clinical Research and Reviews, 16(6):102500. https://doi.org/10.1016/j.dsx.2022.102500
Brosnan, J.T., Drewnowski, A., and Friedman, M.I. (2014). Is there a relationship between dietary MSG and obesity in animals or humans? Amino Acids, 46(9):2,075-2,087. https://doi.org/10.1007/s00726-014-1771-6
Buka, I., Osornio-Vargas, A., and Clark, B. (2011). Food additives, essential nutrients and neurodevelopmental behavioural disorders in children: A brief review. Paediatrics and Child Health, 16(7):e54-e56. https://doi.org/10.1093/pch/16.7.e54
Cai, C., Sivak, A., and Davenport, M.H. (2021). Effects of prenatal artificial sweeteners consumption on birth outcomes: A systematic review and meta-analysis. Public Health Nutrition, 24(15):5,024-5,033. https://doi.org/10.1017/S1368980021000173
Carocho, M., Morales, P., and Ferreira, I.C.F.R. (2017). Sweeteners as food additives in the XXI century: A review of what is known, and what is to come. Food and Chemical Toxicology, 107:302-317. https://doi.org/10.1016/j.fct.2017.06.046
Chakraborty, S.P. (2019). Patho-physiological and toxicological aspects of monosodium glutamate. Toxicology Mechanisms and Methods, 29(6):389-396. https://doi.org/10.1080/15376516.2018.1528649
Chattopadhyay, K. (2017). Ayurveda and lifestyle modification: Research to practice. International Journal of Medicine and Public Health, 7(3):132-133. https://doi.org/10.5530/ijmedph.2017.3.27
Chen, Z.-Y., Li, N.-J., Cheng, F.-Y., Hsueh, J.-F., Huang, C.-C., Lu, F.-I., Fu, T.-F., Yan, S.-J., Lee, Y.-H., and Wang, Y.-J. (2020). The effect of the chorion on size-dependent acute toxicity and underlying mechanisms of amine-modified silver nanoparticles in zebrafish embryos. International Journal of Molecular Sciences, 21(8):2864. https://doi.org/10.3390/ijms21082864
Dewanti, A.I.A., Kunjirika, T.P., Putri, R.R.R.A.D., Adaninggar, A., Raharjeng, A.R.P., Retnoaji, B., Nuriliani, A., Sofyantoro, F., Septriani, N.I., and Saragih, H.T.S.S.G. (2023). Effects of paracetamol on the development of zebrafish (Danio rerio). Pertanika Journal of Tropical Agricultural Science, 46(4):1,173-1,188. https://doi.org/10.47836/pjtas.46.4.06
Durazzo, A., Carocho, M., Heleno, S., Barros, L., Souto, E.B., Santini, A., and Lucarini, M. (2022). Food dyes and health: Literature quantitative research analysis. Measurement: Food, 7:100050. https://doi.org/10.1016/j.meafoo.2022.100050
Freeman, M. (2006). Reconsidering the effects of monosodium glutamate: A literature review. Journal of the American Academy of Nurse Practitioners, 18(10):482-486. https://doi.org/10.1111/j.1745-7599.2006.00160.x
Hamm, J. (2019). Characterizing sources of variability in zebrafish embryo screening protocols. ALTEX, 36(1):103-120. https://doi.org/10.14573/altex.1804162
Jang, W., Jeoung, N.H., and Cho, K.-H. (2011). Modified apolipoprotein (apo) A-I by artificial sweetener causes severe premature cellular senescence and atherosclerosis with impairment of functional and structural properties of apoA-I in lipid-free and lipid-bound states. Molecules and Cells, 31(5), 461-470. https://doi.org/10.1007/s10059-011-1009-3
Kayode, O.T., Bello, J.A., Oguntola, J.A., Kayode, A.A.A., and Olukoya, D.K. (2023). The interplay between monosodium glutamate (MSG) consumption and metabolic disorders. Heliyon, 9(9):e19675. https://doi.org/10.1016/j.heliyon.2023.e19675
Kayode, O.T., Rotimi, D.E., Kayode, A.A.A., Olaolu, T.D., and Adeyemi, O.S. (2020). Monosodium glutamate (MSG)-induced male reproductive dysfunction: A mini review. Toxics, 8(1):7. https://doi.org/10.3390/toxics8010007
Kazmi, Z., Fatima, I., Perveen, S., and Malik, S.S. (2017). Monosodium glutamate: Review on clinical reports. International Journal of Food Properties, 20(sup1):1-9. https://doi.org/10.1080/10942912.2017.1295260
Kim, J.-Y., Seo, J., and Cho, K.-H. (2011). Aspartame-fed zebrafish exhibit acute deaths with swimming defects and saccharin-fed zebrafish have elevation of cholesteryl ester transfer protein activity in hypercholesterolemia. Food and Chemical Toxicology, 49(11):2899-2905. https://doi.org/10.1016/j.fct.2011.08.001
Konrad, S.P., Farah, V., Rodrigues, B., Wichi, R.B., Machado, U.F., Lopes, H.F., Schaan, B.D., De Angelis, K., and Irigoyen, M.C. (2012). Monosodium glutamate neonatal treatment induces cardiovascular autonomic function changes in rodents. Clinics, 67(10):1209-1214. https://doi.org/10.6061/clinics/2012(10)14
Lee, W. and Wang, Y.-C. (2015). Assessing developmental toxicity of caffeine and sweeteners in medaka (Oryzias latipes). SpringerPlus, 4(1):486. https://doi.org/10.1186/s40064-015-1284-0
Lubzens, E., Young, G., Bobe, J., and Cerdà, J. (2010). Oogenesis in teleosts: How fish eggs are formed. General and Comparative Endocrinology, 165(3):367-389. https://doi.org/10.1016/j.ygcen.2009.05.022
Mahmood, A.A.R. and Al-Juboori, S.B. (2020). A review: Saccharin discovery, synthesis, and applications. Iraqi Journal of Pharmaceutical Sciences, 33(2):43. https://doi.org/10.30526/33.2.2442
Mayden, R.L., Tang, K.L., Conway, K.W., Freyhof, J., Chamberlain, S., Haskins, M., Schneider, L., Sudkamp, M., Wood, R.M., Agnew, M., Bufalino, A., Sulaiman, Z., Miya, M., Saitoh, K., and He, S. (2007). Phylogenetic relationships of Danio within the order Cypriniformes: A framework for comparative and evolutionary studies of a model species. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, 308B(5):642-654. https://doi.org/10.1002/jez.b.21175
More, T.A., Shaikh, Z., and Ali, A. (2021). Artificial sweeteners and their health implications: A review. Biosciences Biotechnology Research Asia, 18(2):227-237. https://doi.org/10.13005/bbra/2910
Nakagawa, T., Ukai, K., Ohyama, T., Gomita, Y., and Okamura, H. (2000). Effects of chronic administration of sibutramine on body weight, food intake, and motor activity in neonatally monosodium glutamate-treated obese female rats: Relationship of antiobesity effect with monoamines. Experimental Animals, 49(4):239-249. https://doi.org/10.1538/expanim.49.239
Nakanishi, Y., Tsuneyama, K., Fujimoto, M., Salunga, T.L., Nomoto, K., An, J.-L., Takano, Y., Iizuka, S., Nagata, M., Suzuki, W., Shimada, T., Aburada, M., Nakano, M., Selmi, C., and Gershwin, M.E. (2008). Monosodium glutamate (MSG): A villain and promoter of liver inflammation and dysplasia. Journal of Autoimmunity, 30(1-2):42-50. https://doi.org/10.1016/j.jaut.2007.11.016
Niaz, K., Zaplatic, E., and Spoor, J. (2018). Extensive use of monosodium glutamate: A threat to public health? EXCLI Journal, 17:273. https://doi.org/10.17179/EXCLI2018-1092
Pressman, P., Clemens, R., Hayes, W., and Reddy, C. (2017). Food additive safety: A review of toxicologic and regulatory issues. Toxicology Research and Application, 1:239784731772357. https://doi.org/10.1177/2397847317723572
Raharjeng, A.R.P., Sani, L.P.A., Harahap, J.O., Blatama, D., Pratama, S.F., Adaninggar, A., Nuriliani, A., Retnoaji, B., and Saragih, H.T.S.S. (2022). Effect of chlorpyrifos on embryo development of wader pari fish (Rasbora lateristriata Bleeker, 1854). Biology and Medicine Bulletin, 13(2):21-31. https://doi.org/10.22146/bib.v13i2.4766
Retnoaji, B., Nurhidayat, L., Pratama, S.F., Anshori, K., Hananya, A., Sofyantoro, F., and Bessho, Y. (2023). Embryonic development of Indonesian native fish yellow rasbora (Rasbora lateristriata). Journal of King Saud University - Science, 35(7):102810. https://doi.org/10.1016/j.jksus.2023.102810
Richardson, R., Tracey-White, D., Webster, A., and Moosajee, M. (2017). The zebrafish eye-A paradigm for investigating human ocular genetics. Eye, 31(1):68-86. https://doi.org/10.1038/eye.2016.198
Sambu, S., Hemaram, U., Murugan, R., and Alsofi, A.A. (2022). Toxicological and teratogenic effect of various food additives: An updated review. BioMed Research International, 2022:1-11. https://doi.org/10.1155/2022/6829409
Saputra, F., Lai, Y.-H., Fernandez, R.A.T., Macabeo, A.P.G., Lai, H.-T., Huang, J.-C., and Hsiao, C.-D. (2021). Acute and sub-chronic exposure to artificial sweeteners at the highest environmentally relevant concentration induce less cardiovascular physiology alterations in zebrafish larvae. Biology, 10(6):548. https://doi.org/10.3390/biology10060548
Shuliakevich, A., Schröder, K., Nagengast, L., Muz, M., Pipal, M., Brückner, I., Hilscherova, K., Brack, W., Schiwy, S., and Hollert, H. (2022). Morphological and behavioral alterations in zebrafish larvae after exposure to contaminated river sediments collected in different weather conditions. Science of the Total Environment, 851:157922. https://doi.org/10.1016/j.scitotenv.2022.157922
Stevenson, D.D. (2000). Monosodium glutamate and asthma. Journal of Nutrition, 130(4):1067S-1073S. https://doi.org/10.1093/jn/130.4.1067S
Sun, L., Xin, F., and Alper, H.S. (2021). Biosynthesis of food additives and colorants-a growing trend in future food. Biotechnology Advances, 47:107694. https://doi.org/10.1016/j.biotechadv.2020.107694
Suthamnatpong, N. and Ponpornpisit, A. (2017). Effects of monosodium glutamate on heartbeat and the embryonic development of zebrafish. The Thai Journal of Veterinary Medicine, 47(4):523-530. https://doi.org/10.56808/2985-1130.2865
Valdebenito, I., Figueroa, E., Valdebenito, M., and Paiva, L. (2021). Chorion alterations in eyed-stage salmonid eggs farmed in La Araucanía, Chile: A retrospective study. Animals, 11(8):2427. https://doi.org/10.3390/ani11082427
Vasilaki, A., Panagiotopoulou, E., Koupantsis, T., Katsanidis, E., and Mourtzinos, I. (2022). Recent insights in flavor-enhancers: Definition, mechanism of action, taste-enhancing ingredients, analytical techniques, and the potential of utilization. Critical Reviews in Food Science and Nutrition, 62(32):9,036-9,052. https://doi.org/10.1080/10408398.2021.1939264
Williams, A.N. and Woessner, K.M. (2009). Monosodium glutamate 'allergy': Menace or myth? Clinical and Experimental Allergy, 39(5):640-646. https://doi.org/10.1111/j.1365-2222.2009.03221.x
Wu, L., Zhang, C., Long, Y., Chen, Q., Zhang, W., and Liu, G. (2022). Food additives: From functions to analytical methods. Critical Reviews in Food Science and Nutrition, 62(30):8,497-8,517. https://doi.org/10.1080/10408398.2021.1929823
Zanfirescu, A., Ungurianu, A., Tsatsakis, A.M., Nițulescu, G. M., Kouretas, D., Veskoukis, A., Tsoukalas, D., Engin, A. B., Aschner, M., and Margină, D. (2019). A review of the alleged health hazards of monosodium glutamate. Comprehensive Reviews in Food Science and Food Safety, 18(4):1,111-1,134. https://doi.org/10.1111/1541-4337.12448
