ISOLATION AND SCREENING OF PHOSPHATE AND POTASH SOLUBILIZING BACTERIA FROM RHIZOSPHERE SOIL OF MAIZE AND COTTON
Phosphate and Potash Solubilizing Bacteria in Maize and Cotton Soil
DOI:
https://doi.org/10.55766/sujst3933Keywords:
16S RNA Sequencing, Soil Fertility, Biochemical Characterization, Microbial Solubilization, Nutrient Accessibility, Phosphate and Potash Bacteria, Rhizosphere IsolationAbstract
The availability of soil fertility and growth of plants mainly depends on the interaction of microorganisms with soil nutrients. However, Phosphorus and potassium are indeed essential nutrients for plant growth, but they are often present in the soil as insoluble forms. A microorganism plays a critical role in nutrient mobilization, hence reducing the reliance on chemical fertilizers. The objective of this research is to isolate and screen Phosphate-Solubilizing Bacteria (PSB) and Potash-Solubilizing Bacteria (KSB) from the rhizospheric soil of maize (Zea mays) and cotton (Gossypium herbaceum). 38 different colonies of distinct bacteria were isolated and then screened using Pikovskaya’s agar and Aleksandrow’s agar. Through morphological identification and biochemical characterization, there were 14 isolates with the same morphology as well as 24 unique isolates. Out of them, 12 strains could solubilize phosphate, 10 could dissolve potassium, and 8 could perform both activities simultaneously. The two highest isolates for phosphate-solubilizing efficiency and the three best for potassium dissolution were found by measuring zones of solubilization on agar plates. Those isolates were characterized based on their 16S r RNA sequencing. This research clearly indicates that soil microorganisms play an important role in the availability of nutrients to plates, and provides a way for this dependence on chemical fertilizers to decrease.
References
Aleksandrov, V.G., Blagodyr, R.N., and Ilev, I.P. (1967). Liberation of phosphoric acid from apatite by silicate bacteria. Microchemical Journal, 29:111-114.
Archana, D.S., Nandish, M.S., Savalagi, V.P., and Alagawadi, A. (2012). Screening of potassium solubilizing bacteria (KSB) for plant growth promotional activity. BIOINFOLET, 9(4):627-630.
Azizah, H., Rahajeng, S.M., and Jatmiko, Y.D. (2020). Isolation and screening of phosphate and potassium solubilizing endophytic bacteria in maize (Zea mays L.). Journal of Experimental Life Science, 10(3):165-170. https://doi.org/10.21776/ub.jels.2020.010.03.04
Bashan, Y., Kamnev, A.A., and de-Bashan, L.E. (2013). Tricalcium phosphate is inappropriate as a universal selection factor for isolating and testing phosphate-solubilizing bacteria that enhance plant growth: A proposal for an alternative procedure. Biology and Fertility of Soils, 49(4):465-479. https://doi.org/10.1007/s00374-012-0737-7
Bashan, Y., Rodriguez, T., Gonzalez, I., and Goire, Y.H. (2004). Gluconic acid production and phosphate solubilization by the plant growth-promoting bacterium Azospirillum spp. Naturwissenschaften, 91(9):552-555. https://doi.org/10.1007/s00114-004-0566-0
Bekele Mamo, G., and Gebremedhin, W. (2022). Phosphate solubilizing bacteria in optimizing phosphorus acquisition and crop production. American Journal of Bioscience, 10(2):61-68. https://doi.org/10.11648/j.ajbio.20221002.14
Chabot, R., Antoun, H., and Cescas, M.P. (1993). Stimulation de la croissance du maïs et de la laitue romaine par des microorganismes dissolvant le phosphore inorganique. Canadian Journal of Microbiology, 39(10):941-947. https://doi.org/10.1139/m93-142
Chaskes, S., Frases, S., Cammer, M., Gerfen, G., and Casadevall, A. (2008). Growth and pigment production on D-tryptophan medium by Cryptococcus gattii, Cryptococcus neoformans, and Candida albicans. Journal of Clinical Microbiology, 46(1):255-264. https://doi.org/10.1128/JCM.01721-07
Coico, R. (2006). Gram staining. Current Protocols in Microbiology, 00(1):A.3C.1-A.3C.2. https://doi.org/10.1002/9780471729259.mca03cs00
Cullen, J.J., and MacIntyre, H.L. (2016). On the use of the serial dilution culture method to enumerate viable phytoplankton in natural communities of plankton subjected to ballast water treatment. Journal of Applied Phycology, 28(1):279-298. https://doi.org/10.1007/s10811-015-0601-x
Darlene, B., and Sabey, B.R. (1976). Use of a microwave oven for moisture determination in a soil science laboratory. Supported by the Colorado State University Experiment Station, Publication Science S, Paper No. 2159.
Daroonkolaei, S., Amerian, M., Pirdashti, H., Bakhshandeh, E., and Gholami, A. (2022). Evaluation of the impact of potassium solubilizing bacteria on potassium efficiency and yield of canola under saline and non-saline soil conditions. Archives of Agronomy and Soil Science, 69(1):1-17. https://doi.org/10.1080/03650340.2022.2096217
Deepak, P., and Chayanika, P. (2018). Isolation and characterization of phosphate solubilizing bacteria from the rhizosphere of potato plant. International Journal of Current Microbiology and Applied Sciences, 7(1):967-975. https://doi.org/10.20546/ijcmas.2018.701.116
Feng, S., Ou, Q., Wang, N., Guo, Z., Ou, Y., Li, N., and Peng, C. (2020). Isolation and identification of potassium-solubilizing bacteria from Mikania micrantha rhizospheric soil and their effect on M. micrantha plants. Global Ecology and Conservation, 23:e01076. https://doi.org/10.1016/j.gecco.2020.e01141
Ganesh Moorthy, S.R., Kumar, S.S., Devandaran, K., Anguchamy, S., Ragunathan, R., and Johney, J. (2024). Evaluation of the antimicrobial, antioxidant, and cytotoxicity against MCF-7 breast cancer cell lines of Phyllanthus emblica L. bark extract. Journal of Natural Remedies, 24(5):1091-1097. https://doi.org/10.18311/jnr/2024/36228
Goldstein, A.H. (1994). Involvement of the quinoprotein glucose dehydrogenase in the solubilization of exogenous phosphates by gram-negative bacteria. In A. Torriani-Gorini, E. Yagil, and S. Silver (Eds.), Phosphate in microorganisms: Cellular and molecular biology (pp. 197-203). ASM Press.
Gram, H.C. (1884). über die isolierte Farbung der Schizomyceten in Schnittund Trocken praparaten. Fortschritte der Medizin, 2:185-189.
Halder, A.K., Mishra, A.K., Bhattacharyya, P., and Chakrabartty, P.K. (1990). Solubilization of rock phosphate by Rhizobium and Bradyrhizobium. Journal of General and Applied Microbiology, 36(2):81-92. https://doi.org/10.2323/jgam.36.81
He, L.Y., Zhang, Y.F., Ma, H.Y., Su, L.N., Chen, Z.J., Wang, Q.Y., Qian, M., and Sheng, X.F. (2010). Characterization of copper-resistant bacteria and assessment of bacterial communities in rhizosphere soils of copper-tolerant plants. Applied Soil Ecology, 44(1):49-55. https://doi.org/10.1016/j.apsoil.2009.09.004
Jacoby, R., Peukert, M., Succurro, A., Koprivova, A., and Kopriva, S. (2017). The role of soil microorganisms in plant mineral nutrition-Current knowledge and future directions. Frontiers in Plant Science, 8:1617. https://doi.org/10.3389/fpls.2017.01617
Kandi, V. (2015). Bacterial colony: First report of donut colony morphology among diphtheroids isolated in blood. Cureus, 7(11). https://doi.org/10.7759/cureus.374
Krauss, A. (2003). The International Potash Institute in retrospect and prospect. In J. E. Johnston (Ed.), Proceedings of the IPI Golden Jubilee Congress 1952-2002: Invited papers “Feed the soil to feed the people. The role of potash in sustainable agriculture” (Vol. 1, pp. 11-22). International Potash Institute.
Mazhar, S., Kilcawley, K.N., Hill, C., and McAuliffe, O. (2020). A systems-wide analysis of proteolytic and lipolytic pathways uncovers the flavor-forming potential of the Gram-positive bacterium Macrococcus caseolyticus subsp. caseolyticus. Frontiers in Microbiology, 11:1533. https://doi.org/10.3389/fmicb.2020.01533
Meena, M.A., Maurya, B.R., Verma, J.P., Aeron, A., Kumar, A., Kim, K., and Bajpai, V.K. (2015). Potassium solubilizing rhizobacteria (KSR): Isolation, identification, and K-release dynamics from waste mica. Ecological Engineering, 81:340-347. https://doi.org/10.1016/j.ecoleng.2015.04.065
Pal, S.S. (1998). Interaction of an acid tolerant strain of phosphate solubilizing bacteria with a few acid tolerant crops. Plant and Soil, 200(1):169-177. https://doi.org/10.1023/A:1004318814385
Rajawat, M.V.S., Ansari, W.A., Singh, D., and Singh, R. (2019). Potassium solubilizing bacteria (KSB). In D. P. Singh & R. Prabha (Eds.), Microbial interventions in agriculture and environment: Volume 3: Soil and crop health management (pp. 189-209). Springer Singapore. https://doi.org/10.1007/978-981-32-9084-6_9
Rodríguez, H., and Fraga, R. (1999). Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances, 17(4-5):319-339. https://doi.org/10.1016/S0734-9750(99)00014-2
Römheld, V., and Kirkby, E.A. (2010). Research on potassium in agriculture: Needs and prospects. Plant and Soil, 335(1-2):155-180. https://doi.org/10.1007/s11104-010-0520-1
Sharma, S.B., Sayyed, R.Z., Trivedi, M.H., and Gobi, T.A. (2013). Phosphate solubilizing microbes: Sustainable approach for managing phosphorus deficiency in agricultural soils. SpringerPlus, 2(1):587. https://doi.org/10.1186/2193-1801-2-587
Sun, F., Ou, Q., Wang, N., Guo, Z., and Ou, Y. (2020). Isolation and identification of potassium-solubilizing bacteria from Mikania micrantha rhizospheric soil and their effect on M. micrantha plants. Global Ecology and Conservation, 23:e01141. https://doi.org/10.1016/j.gecco.2020.e01141
Vassilev, N., and Vassileva, M. (2003). Biotechnological solubilization of rock phosphate on media containing agro-industrial wastes. Applied Microbiology and Biotechnology, 61(5-6):435-440. https://doi.org/10.1007/s00253-003-1318-3
Verma, D.K., Vasudeva, G., Sidhu, C., Pinnaka, A.K., Prasad, S.E., and Thakur, K.G. (2020). Biochemical and Taxonomic Characterization of Novel Haloarchaeal Strains and Purification of the Recombinant Halotolerant α-Amylase Discovered in the Isolate. Frontiers in Microbiology, 11:2082. https://doi.org/10.3389/fmicb.2020.02082
White, P.J., and Brown, P.H. (2010). Plant nutrition for sustainable development and global health. Annals of Botany, 105(7):1073-1080. https://doi.org/10.1093/aob/mcq085
Yassin, S.N., Jiru, T.M., and Indracanti, M. (2021). Screening and characterization of thermostable amylase-producing bacteria isolated from soil samples of Afdera, Afar region, and molecular detection of amylase-coding gene. International Journal of Microbiology, 2021:5592885. https://doi.org/10.1155/2021/5592885
Zhang, C., Maurya, B.R., Verma, J.P., and Kong, F. (2014). Isolation and identification of potassium-solubilizing bacteria from tobacco rhizospheric soil and their effect on tobacco plants. Applied Soil Ecology, 82:18-25. https://doi.org/10.1016/j.apsoil.2014.05.002
