FACTORIAL DESIGN OPTIMIZATION OF PHENOLIC AND ANTIOXIDANT EXTRACTION FROM Justicia gendarussa Burm.F.
Optimizing Phenolic and Antioxidant Extraction from Justicia gendarussa
DOI:
https://doi.org/10.55766/sujst-2024-03-e03434Keywords:
antioxidant, factorial, Justicia gendarussa, phenolicAbstract
Extraction is a pivotal initial step in the process of harnessing active constituents from plants. The extraction process aims to efficiently yield the highest concentration of target compounds. This study focused on optimizing the extraction of phenolic compounds and antioxidant capacity from Justicia gendarussa Burm.f., a plant esteemed for its medicinal properties. A two-level half-factorial design was utilized to systematically investigate the effects of the liquid-to-solid ratio, temperature, solvent concentration, time, and pH on the extraction yields. The study determined that the optimal extraction was achieved in run-13, where the total phenolic content reached 22.75 mg GAE/g, and the antioxidant capacities measured by DPPH and FRAP assays were 43.36 μmol TE/g and 166.72 μmol TE/g, respectively. Temperature was found to be a significant factor influencing phenolic production, whereas the liquid-to-solid ratio and pH had significant effects on the antioxidant capacity of DPPH, according to statistical analysis. Similarly, the liquid-to-solid ratio is crucial for the antioxidant capacity of FRAP. Optimal extraction parameters were identified with a liquid-to-solid ratio of 93.7 mL/g, temperature of 43.4°C, methanol concentration of 60.6%, extraction time of 113 min, and pH of 3.45, leading to the most effective extraction process with a desirability level of 1. The results of this study provide a valuable methodology for enhancing the extraction efficiency of bioactive compounds from J. gendarussa, thus supporting its potential for pharmaceutical utilization.
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