BIOREMEDIATION OF PLASTICS BY MICROORGANISMS
DOI:
https://doi.org/10.55766/sujst-2024-03-e03377Keywords:
Bioremediation, Degradation, Microorganisms, PlasticsAbstract
Soil is an inherent medium that sustains life on our planet. Soil serves as a foundation that is essential for the development and sustenance of life on our planet. It functions as a storage area for air, water, and nutrients that are crucial for the development of living creatures, particularly vegetation and microbes. The nutrient content of the soil is determined by the composition and abundance of microorganisms that reside within it. Plastic pollution is a significant form of pollution that poses a continuing hazard to the entire ecosystem due to its ongoing deposition in the environment. The current research focuses on the degradation of plastic through the method of bioremediation using microorganisms isolated from the garden soil taken from St. Ann’s College for Women in Mehdipatnam, Hyderabad. Fungal extraction has been conducted, and shaker flask studies have been performed to assess the fungi’s capacity for degrading plastics. The bioremediation process was evaluated by measuring the weight loss caused by various microorganisms. The bioremediation experiments were conducted over a period of 90 days using a potato dextrose agar medium (PDA). The microbes which degraded the plastics are Aspergillus species and Fusarium species. The percentage of degradation was found to be 25%.
References
Adesemoye, A.O., Opere, B.O., and Makinde, S.C.O. (2006) Microbial content of abattoir wastewater and its contaminated soil in Lagos, Nigeria. African Journal of biotechnology, 5(20):1963-1968.
Ahsan, A., Ashraf, M., Aslam, R., Zubair., M.H. Rahman, S.U., and Ail, S. (2016). Isolation and screening of polyethylene degrading fungi from solid waste material. Journal of Agriculture And Basic Sciences, 1(1):1-6.
Ayeni, T.O., Arotupin, D.J., and Ayo, O.E. (2022). Biodegradation of polyethylene by indigenous fungi from waste recycling site, South West, Nigeria. Bulletin of the national Research Centre, 46(1):182. https://doi.org/10.1186/s42269-022-00871-4.
Bryant, J.A., Clemente, T.M., Viviani, D.A., Fong, A.A., Thomas, K.A., kemp, P., Karl, D.M., White, A.E., and DeLong, E.F. (2016). Diversity and Activity of Communities Inhabiting Plastic Debris in the North Pacific Gyre. mSystems, 1:e00024-16. https://doi.org/10.1128/mSystems.00024-16
Caruso, G. (2015). Plastic degrading microorganisms as a tool for bioremediation of plastic contamination in aquatic environments. Journal of Pollution Effects & Control, 3:1000e112. https://doi.org/10.4172/2375-4397.1000e112
Das, M. and Adholeya, A. (2012). Role of Microorganisms in Remediation of Contaminated Soil. In: Satyanarayana, T., Johri, B. (eds) Microorganisms in Environmental Management. Springer, Dordrecht. p. 81-111. https://doi.org/10.1007/978-94-007-2229-3_4
Deepika, S. and Madhuri, J.R. (2015) Biodegradation of Low-Density Polyethylene by Microorganisms from Garbage Soil. Journal of Experimental Biology and Agriculture Sciences, 3(1):15-21.
Gajendiran, A., Krishnamoorthy, S., and Abraham, J. (2016). Microbial degradation of low-density polyethylene (LDPE) by Aspergillus clavatus strain JASK1 isolated from landfill soil. 3Biotech, 6(1):52. https://doi.org/10.1007/s13205-016-0394-x
Glanz, J. (1995). Saving our soil: solutions for sustaining earth's vital resource. Boulder, USA, Johnson Printing.
Gong, Z., Jin, L., Yu, X., Wang, B., Hu, S., Ruan, H., Sung, Y-J., Lee, H-G., and Jin, F. (2023). Biodegradation of Low Density Polyethylene by the Fungus Cladosporium sp. Recovered from a Landfill Site. Journal of Fungi, 9(6):605. https://doi.org/10.3390/jof9060605
Horton, A.A., Walton, A., Spurgeon, D.J., Lahive, E., and Svendsen, C. (2017). Microplastics in freshwater and terrestrial environments: evaluating the current understanding to identify the knowledge gaps and future research priorities. Science of The Total Environment, 586:127-141. https://doi.org/10.1016/j.scitotenv.2017.01.190
Ishii, N., Inoue, Y., Shimada, K-I., Tezuka, Y., Mitomo, H., and Kasuya, K-I. (2007). Fungal degradation of poly (ethylene succinate). Polymer Degradation and Stability, 92(1):44-52. https://doi.org/10.1016/j.polymdegradstab.2006.09.014
Khruengsai, S., Sripahco, T., Pripdeevech, P. (2021). Low-Density Polythene Film Biodegradation potential by Fungal Species from Thailand. Journal of Fungi, 7(8):594. https://doi.org/10.3390/jof7080594
Kyaw, B.M., Champakalakshmi, R., Sakharkar, M.K., Lim, C.S., and Sakharkar, K.R. (2012). Biodegradation of low density polythene (LDPE) by Pseudomonas species. Indian Journal of Microbiology, 52(3):411-419. https://doi.org/10.1007/s12088-012-0250-6
Lal, R., Monger, C., Nave, L., Smith, P. (2021). The role of soil in regulation of climate. Philosophical Transactions of The Royal Society B: Biological Sciences, 376(1838):20210420. https://doi.org/10.1098/rstb.2021.0084
Mahdiyah, D. and Mukti, B.H. (2013) Isolation of Polyethylene Plastic Degrading-Bacteria. Biosciences International, 2:29-32.
Mueller, R-J. (2006). Biological degradation of synthetic polyesters-Enzymes as potential catalysts for polyester recycling. Process Biochemistry, 41(10):2124-2128. https://doi.org/10.1016/j.procbio.2006.05.018
Nizzetto, L., Futter, M., and Langaas, S. (2016): Are agricultural soils dumps for microplastics of urban origin?. Environmental Science and Technology, 50(20):10777-10779. https://doi.org/10.1021/acs.est.6b04140
Norman, M.J.T., Pearson, C.J., and Searle, P.G.E. (1995). The Ecology of Tropical Food Crops. 2nd ed. Cambridge University Press. p. 249-251. https://doi.org/10.1017/CBO9781139172479
Ogunbayo, A.O., Olanipekun, O.O., and Adamu, I.A. (2019) Preliminary studies on the Microbial Degradation of Plastic Waste Using Aspergillus niger and Pseudomonas sp. Journal of Environmental Protection, 10(5):625-631. https://doi.org/10.4236/jep.2019.105037
Olicon-Hernandez, D.R., Gonzalez-Lopez, J., and Aranda, E. (2017). Overview on the Biochemical Potential of Filamentous Fungi to Degrade Pharmaceutical Compounds. Frontiers in Microbiology, 8:1792. https://doi.org/10.3389/fmicb.2017.01792
Orr, I.G., Hadar, y., and Sivan, A. (2004). Colonization, biofilm formation and biodegradation of polyethylene by a strain of Rhodococcus ruber. Applied Microbiology and Biotechnology, 65(1):97-104. https://doi.org/10.1007/s00253-004-1584-8
Ponniah, S., Anandan, S., Kaliappan, N., Thangavelu, S., and Veerapandiyan, T. (2014) Biodegradation of Plastics by Pseudomonas Putida Isolated from Garden Soil Samples. Journal of Advance Botany and Zoology, 1(3):2348-7313.
Roy, P.K., Titus, S., Surekha, P., Tulsi, E., Deshmukh, C., and Rajagopal, C. (2008). Degradation of abiotically aged LDPE films containing pro-oxidant by bacterial consortium. Polymer Degradation and Stability, 93(10):1917-1922. https://doi.org/10.1016/j.polymdegradstab.2008.07.016
Sanchez, C. (2020). Fungal potential for the degradation of petroleum-based polymers: An overview of macro- and microplastics biodegradation. Biotechnology Advances, 40:107501. https://doi.org/10.1016/j.biotechadv.2019.107501
Sivasankari, S. and Vinotha, T. (2014). In Vitro Degradation of Plastics (Plastic Cup) Using Micrococcus Luteus and Masoniella Sp. Scholar Acadademic Journal of Biosciences, 2(2):85-89. https://doi.org/10.36347/sajb.2014.v02i02.004
Sridharan, K. and Ji, S. (2023). Climate Change Impacts on the Built Heritage of Hyderabad. In: Chakrabarti, A., Singh, V. (eds) Design in the Era of Industry 4.0, Volume 2. ICORD 2023. Smart Innovation, Systems and Technologies, 342:1087-1011. https://doi.org/10.1007/978-981-99-0264-4_89
Strong, A.B. (2006) Plastics: Materials and Processing. 3rd Edition, PEARSON Prentice Hall, Upper saddle River, NJ.
Sumathi, T., Viswanath, B., Sri Lakshmi, A., and SaiGopal, D.V. (2016). Production of Laccase by Cochliobolus sp. Isolated from Plastic Dumped Soils and Their Ability to Degrade Low Molecular Weight PVC. Biochemistry Research International, 2016(1):9519527. https://doi.org/10.1155/2016/9519527
Varshney, S., Gupta, V., Yadav, A.N., Rahi, R.K., Devki,. and Neelam, D.K. (2023). An overview on role of fungi in systematic plastic degradation. Journal of Applied Biology and Biotechnology, 11(3):61-69. https://doi.org/10.7324/JABB.2023.108929
Yoon, M.G., Jeon, H.J., and Kim, M.N. (2012). Biodegradation of Polyethylene by a Soil Bacterium and AlkB Cloned Recombinant Cell, Journal of Bioremediation & Biodegradation, 3(4):145. https://doi.org/10.4172/2155-6199.1000145
Yu, J.R., Adingo, S., Liu, X.L., Li, X.D., Sun, J., and Zhang X.N. (2022). Micro plastics in soil ecosystem - A review of sources, fate, and ecological impact. Plant, Soil and Environment, 68(1):1-17. https://doi.org/10.17221/242/2021-PSE
Zahra, S., Abbas, S.S., Mahsa, M-T., and Mohsen, N. (2010). Biodegradation of low-density polyethylene(LDPE) by isolated fungi in solid waste medium. Waste Management, 30(3):396-401. https://doi.org/10.1016/j.wasman.2009.09.027
Zettler, E.R., Mincer, T.J., and Amaral-Zettler, L.A. (2013). Life in the “Plastisphere”: Microbial Communities on Plastic Marine Debris. Environmental Science & Technology, 47(13):7137-7146. https://doi.org/10.1021/es401288x








