OPTIMIZATION OF LEGUME BASED MEAT ALTERNATIVE USING FREEZE STRUCTURING AND EVALUATION OF ITS QUALITY INDICES

Authors

  • Devanampriyan Rajan -Kongu Engineering college
  • Chitra Devi Venkatachalam
  • Sangeetha Arunachalam

DOI:

https://doi.org/10.55766/sujst-2024-01-e02685

Keywords:

meat analogues, Protein isolates, Freeze structuring, Fibrous structure, Textural attributes

Abstract

The continuous development and increasing demand of the fast-growing market for meat alternatives will offer abundant opportunities for food scientists and entrepreneurs to innovate new technologies. Product development and applied research primarily focus on the sensory qualities related to the texture of meat analogues. The preference for sustainable plant-based meat over animal meat worldwide is primarily driven by the growing concerns regarding the environment and health. In this study, various plant-based composites were investigated to create plant-based meat alternatives with distinctive texture profiles using an innovative freeze-structuring technique. Statistical ANOVA was utilized to optimize the composite consisting of pea proteins isolate (PPI) and soy protein isolate (ISP). The physicochemical, textural, and sensory properties of the analogs were thoroughly examined. The findings indicated that the analog with a ratio of 40:60 (PPI:ISP) was the most favored compared to other formulations. The incorporation of soy protein seemed to have an impact on the textural properties. The optimized composition level (40:60) exhibited a hardness of 842.87g, chewiness of 721.35 N/cm, and the highest sensory properties on an 8.3 hedonic scale. Additionally, the water activity was measured to be 0.553, classifying the product as an intermediate moisture food. The nutritional analysis shows that optimized meat substitutes have 16 % increased protein levels when compared to conventional chicken meat. This study improved the comprehension of the impacts of composites on the structure and physicochemical characteristics of plant-based meat substitutes. It highlighted the potential of utilizing plant protein composites in the development of satisfactory meat analogues.

References

Ahmad, M., Qureshi, S., Akbar, M.H., Siddiqui, S.A., Gani, A., Mushtaq, M., Hassan, I., and Dhull, S.B. (2022). Plant-based meat alternatives: Compositional analysis, current development and challenges. Applied Food Research, 2(2):100154. https://doi.org/10.1016/j.afres.2022.100154.

Aktas, N. and Genccelep, H. (2006). Effect of starch type and its modifications on physicochemical properties of bologna-type sausage produced with sheep tail fat. Meat Science, 74(2):404-408. https://doi.org/10.1016/j.meatsci.2006.04.012.

Alvarez, D., Delles, R.M., Xiong, Y.L., Castillo, M., Payne, F.A., and Laencina, J. (2011). Influence of canola-olive oils, rice bran and walnut on functionality and emulsion stability of frankfurters. LWT-Food Science and Technology, 44(6):1435-1442. https://doi.org/10.1016/j.lwt.2011.01.006.

Amazon. (2023a). Search by pea protein isolate powder: Amazon India. Available from: https://amzn.eu/d/8KdwXOq. Accessed date: August 18, 2023.

Amazon. (2023b). Search by Vital Wheat Gluten powder: Amazon India. Available from: https://amzn.eu/d/hqjUr4a. Accessed date: September 20, 2023

Amazon. (2023c). Search by soy protein isolate powder: Amazon India. Available from: https://amzn.eu/d/dkFHGNZ. Accessed date: August 18, 2023.

Artz, W.E., Osidacz, P.C., and Coscione, A.R. (2005). Iron accumulation in oil during the deep‐fat frying of meat. Journal of the American Oil Chemists’ Society, 82(4):249. https://doi.org/10.1007/s11746-005-1063-8.

Asgar, M., Fazilah, A., Huda, N., Bhat, R., and Karim, A. (2010). Nonmeat protein alternatives as meat extenders and meat analogs. Comprehensive Reviews in Food Science and Food Safety, 9(5):513-529. https://doi.org/10.1111/j.1541-4337.2010.00124.x.

Bader, S., Bez, J., and Eisner, P. (2011). Can protein functionalities be enhanced by high-pressure homogenization?-A study on functional properties of lupin proteins. Procedia Food Science, 1:1359-1366. https://doi.org/10.1016/j.profoo.2011.09.201.

Bakhsh, A., Lee, S.J., Lee, E.Y., Sabikun, N., Hwang, Y.H., and Joo, S.T. (2021). A novel approach for tuning the physicochemical, textural, and sensory characteristics of plant-based meat analogs with different levels of methylcellulose concentration. Foods, 10(3), 560. https://doi.org/10.3390/foods10030560.

Biscarra-Bellio, J.C., de Oliveira, G.B., Marques, M.C., and Molento, C.F. (2023). Demand changes meat as changing meat reshapes demand: The great meat revolution. Meat Science, 196:109040. https://doi.org/10.1016/j.meatsci.2022.109040.

Chantanuson, R., Nagamine, S., Kobayashi, T., and Nakagawa, K. (2022). Preparation of soy protein-based food gels and control of fibrous structure and rheological property by freezing. Food Structure, 32:100258. https://doi.org/10.1016/j.foostr.2022.100258.

Cheftel, J., Kitagawa, M., and Queguiner, C. (1992). New protein texturization processes by extrusion cooking at high moisture levels. Food Reviews International, 8(2):235-275. https://doi.org/10.1080/87559129209540940.

Chitradevi,V. and Devanampriyan, R. (2023). Optimization of legume based composite meat alternatives ad evaluation of its quality indices. Conference theme on Millets as a nutritional paradigm for ensuring food safety, September 14-15, 2023; College of food and dairy technology, Chennai, Tamilnadu, p.172.

Consolacion, F.I. and Jelen, P. (1986). Freeze texturation of proteins: Effect of the alkali, acid and freezing treatments on texture formation. Food Structure, 5(1):5.

Das, S.K., Prabhakaran, P., Tanwar, V.K., and Biswas, S. (2015). Effect of some plant starches and carrageenan as fat substitutes in chicken patties. Journal of animal science, 93(7):3704-3712. https://doi.org/10.2527/jas.2013-6667.

De Angelis, D., Kaleda, A., Pasqualone, A., Vaikma, H., Tamm, M., Tammik, M.-L., Squeo, G., and Summo, C. (2020). Physicochemical and sensorial evaluation of meat analogues produced from dry-fractionated pea and oat proteins. Foods, 9(12):1754. https://doi.org/10.3390/foods9121754.

Dekkers, B.L., Boom, R.M., and van der Goot, A.J. (2018). Structuring processes for meat analogues. Trends in Food Science & Technology, 81:25-36. https://doi.org/10.1016/j.tifs.2018.08.011.

Devanampriyan, R., Sangeetha, A., and Harini, A. (2023). Effect of Textural Properties on Prawn Chips Using Image Processing Technique. Journal of Food and Dietetics Research, 3(1):10-14.

Diaz, J.R., Kantanen, K., Edelmann, J.M., Suhonen, H., Sontag-Strohm, T., Jouppila, K., and Piironen, V. (2022). Fibrous meat analogues containing oat fiber concentrate and pea protein isolate: Mechanical and physicochemical characterization. Innovative Food Science & Emerging Technologies, 77:102954. https://doi.org/10.1016/j.ifset.2022.102954.

Don, C., Lichtendonk, W., Plijter, J., and Hamer, R. (2003). Glutenin macropolymer: a gel formed by glutenin particles. Journal of Cereal Science, 37(1):1-7. https://doi.org/10.1006/jcrs.2002.0481.

Fenton, T., Kanyuck, K., Mills, T., and Pelan, E. (2021). Formulation and characterisation of kappa-carrageenan gels with non-ionic surfactant for melting-triggered controlled release. Carbohydrate Polymer Technologies and Applications, 2:100060. https://doi.org/10.1016/j.carpta.2021.100060

Flores Llovera, M., Hernán, A., Salvador, A., and Belloch, C. (2023). Experimental Data of manuscript Influence of soaking and solvent extraction for deodorization of texturized pea protein isolate on the formulation and properties of hybrid meat patties. https://doi.org/10.1002/jsfa.12453.

Frank, D., Oytam, Y., Hughes, J., McDonnell, C.K., and Buckow, R. (2022). Chapter 31 - Sensory perceptions and new consumer attitudes to meat, Editor(s): Peter Purslow, In Woodhead Publishing Series in Food Science, Technology and Nutrition, New Aspects of Meat Quality (Second Edition), Woodhead Publishing, p. 853-886. https://doi.org/10.1016/B978-0-323-85879-3.00016-7.

Galina, G., Irina, P., Sergey, S., Alesya, G., Oksana, A., Olga, M., and Marina, P. (2020). Biological value of semismoked sausages with cedar oil cake. Foods and Raw materials, 8(1), 30-39. https://doi.org/10.21603/2308-4057-2020-1-30-39.

Geerts, M.E., Dekkers, B.L., van der Padt, A., and van der Goot, A.J. (2018). Aqueous fractionation processes of soy protein for fibrous structure formation. Innovative Food Science & Emerging Technologies, 45:313-319. https://doi.org/10.1016/j.ifset.2017.12.002.

Giridharaprasad, S., Ravi, D.K., Miller, K., and Rajoo, B. (2021). Optimization of incorporating κ‐Carrageenan‐based gels on improving cloud stability, physical stability, and viscosity of ready‐to‐drink mango juice. Journal of Food Science, 86(9):4017-4025. https://doi.org/10.1111/1750-3841.15874.

Hamid, M.A., Tsia, F.L.C., Okit, A.A.B., Xin, C.W., Cien, H.H., Harn, L.S., Patrick, P.N., Samirin, S., Azizi, W.A.A.W., Irfanian, A., and Yee, C.F. (2020). The application of Jackfruit by-product on the development of healthy meat analogue. In IOP Conference Series: Earth and Environmental Science, 575(1):012001. https://doi.org/10.1088/1755-1315/575/1/012001.

Herbivo. (2023). Search by Good Dot Meatless mince kit: Herbivo India. Available from: https://herbivo.in/product/gooddot-meatless-mince-kit/.Accessed date: October, 17, 2023.

Kitcharoenthawornchai, N. and Harnsilawat, T. (2015). Characterization of meat analogue nugget: effect of textured vegetable protein. Food and Applied Bioscience Journal, 3(2):121-129.

Kolaric, L. and Šimko, P. (2020). The comparison of HPLC and spectrophotometric method for cholesterol determination. Potravinarstvo, 14(1):118-124. https://doi.org/10.5219/1302.

Lee, J.S., Kim, S., Jeong, Y.J., Choi, I., and Han, J. (2023). Impact of interactions between soy and pea proteins on quality characteristics of high-moisture meat analogues prepared via extrusion cooking process. Food Hydrocolloids, 139:108567. https://doi.org/10.1016/j.foodhyd.2023.108567.

McClements, D.J., Weiss, J., Kinchla, A.J., Nolden, A.A., and Grossmann, L. (2021). Methods for testing the quality attributes of plant-based foods: Meat-and processed-meat analogs. Foods, 10(2):260. https://doi.org/10.3390/foods10020260.

Mintel. (2023). Search by ingredient complies: Mintel India: Availablefrom:https://www.gnpd.com/sinatra/analysis/cha rt_results/sear ch/FyS6DqJvk0/? analysis_id=216f0be4- 7bcd-4179-9c4a- b28281ccb780&current_tab=216f0be4- 7bcd-4179-9c4a- b28281ccb780/. Accessed date: October, 10, 2023.

Muhialdin, B.J. and Ubbink, J. (2023). Effects of pH and aging on the texture and physicochemical properties of extruded pea protein isolate. Food Hydrocolloids, 140:108639. https://doi.org/10.1016/j.foodhyd.2023.108639.

Patrakova, I.S. and starkov, V.V. (2023). Social innovation - reduced sodium meat products. p. 325-327. https://doi.org/10.21603/-I-IC-101.

Pietsch, V.L., Karbstein, H.P., and Emin, M.A. (2018). Kinetics of wheat gluten polymerization at extrusion-like conditions relevant for the production of meat analog products. Food Hydrocolloids, 85:102-109. https://doi.org/10.1016/j.foodhyd.2018.07.008.

Profeta, A., Baune, M. C., Smetana, S., Broucke, K., Van Royen, G., Weiss, J., Hieke, S., Heinz, V., and and Terjung, N. (2021). Consumer preferences for meat blended with plant proteins-Empirical findings from Belgium. Future Foods, 4:100088. https://doi.org/10.1016/j.fufo.2021.100088.

Rajan, D., Arunachalam, S., Subramani, D., Alagumurugan, H., and Ganesan, A. (2023). Green foods as sustainable meat alternative: a review. Suranaree Journal of Science and Technology, 30(5):020024(1-12). https://doi.org/10.55766/sujst-2023-05-e0154.

Raney, T., Skoet, J., and Steinfeld, H. (2009). Communications division, Food and Agriculture Organization of the United Nations. The state of food and agriculture: livestock in the balance. FAO, Rome. 166p.

Rareunrom, K., Tongta, S., and Yongsawatdigul, J. (2008). Effects of soy protein isolate on chemical and physical characteristics of meat analog. Asian Journal of Food and Agro-Industry, 1(2):99-106.

Riascos, J.J., Weissinger, A.K., Weissinger, S.M., and Burks, A.W. (2010). Hypoallergenic legume crops and food allergy: factors affecting feasibility and risk. Journal of agricultural and food chemistry, 58(1):20-27. https://doi.org/10.1021/jf902526y.

Sangeetha, A., Devanampriyan, R., Harini, A., and Sairagul, G. (2023). Optimization and formulation of prawn to develop a substitute for coarse spice powder. Biochemical & Cellular Archives, 23(2):775. https://doi.org/10.51470/bca.2023.23.2.775.

Sha, L. and Xiong, Y.L. (2020). Plant protein-based alternatives of reconstructed meat: Science, technology, and challenges. Trends in Food Science & Technology, 102:51-61. https://doi.org/10.1016/j.tifs.2020.05.022.

Siong, T.E., Choo, K.S., and Shahid, S.M. (1989). Determination of iron in foods by the atomic absorption spectrophotometric and colorimetric methods. Pertanika, 12(3):313-322.

Subramani, D., Kumaraguruparaswami, M., Muthusamy, H., Arunachalam, S., and Shanmugam, G. (2022). Formulation and quality evaluation of quinoa enriched ready to cook string hoppers (Indian traditional noodles). Journal of Culinary Science & Technology, 1-20. https://doi.org/10.1080/15428052.2022.2040680.

Sun, X.D. and Arntfield, S.D. (2012). Molecular forces involved in heat-induced pea protein gelation: Effects of various reagents on the rheological properties of salt-extracted pea protein gels. Food Hydrocolloids, 28(2):325-332. https://doi.org/10.1016/j.foodhyd.2011.12.014.

Taghian D.S., Charles Carrillo, M.F., Boom, R., and van der Goot, A.J. (2023). Quality improvement of plant-based meat alternatives by addition of iota carrageenan to pea protein-wheat gluten blend. European Food Research and Technology, 249(6):1637-1654. https://doi.org/10.1007/s00217-023-04244-7.

Vernaza, M.G. and Chang, Y.K. (2020). Amido resistente e isolado proteico de soja na produção de macarrão instantâneo obtido pelo processo de fritura convencional e fritura a vácuo. Brazilian Journal of Food Technology, 23.

Webb, D., Dogan, H., Li, Y., and Alavi, S. (2023). Physico-Chemical Properties and Texturization of Pea, Wheat and Soy Proteins Using Extrusion and Their Application in Plant-Based Meat. Foods, 12(8):1586. https://doi.org/10.3390/foods12081586.

Yuliarti, O., Kovis, T.J.K., and Yi, N.J. (2021). Structuring the meat analogue by using plant-based derived composites. Journal of Food Engineering, 288:110138. https://doi.org/10.1016/j.jfoodeng.2020.110138.

Zhang, R., Yang, Y., Liu, Q., Xu, L., Bao, H., Ren, X., Jin, Z., and Jiao, A. (2023). Effect of Wheat Gluten and Peanut Protein Ratio on the Moisture Distribution and Textural Quality of High-Moisture Extruded Meat Analogs from an Extruder Response Perspective. Foods, 12(8):1696. https://doi.org/10.3390/foods12081696.

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Published

2024-03-15

How to Cite

Rajan, D., Chitra Devi Venkatachalam, & Sangeetha Arunachalam. (2024). OPTIMIZATION OF LEGUME BASED MEAT ALTERNATIVE USING FREEZE STRUCTURING AND EVALUATION OF ITS QUALITY INDICES. Suranaree Journal of Science and Technology, 31(1), 020029(1–14). https://doi.org/10.55766/sujst-2024-01-e02685

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