INCREASING GROWTH RATE OF Botryocuccus braunii USING ULTRASONIC WAVES

Authors

  • Asleena Salaeh Division of Physics, School of Science, Walailak University, Thasala, Nakhon Si Thammarat, Thailand 80160

DOI:

https://doi.org/10.55766/sujst-2024-01-e02492

Keywords:

Biomass productivity, Botryocuccus braunii, specific growth rate, ultrasonic parameters

Abstract

Microalgae have emerged as a promising alternative biomass source, offering distinct advantages over conventional crops in terms of oil content per mass. Maximizing their reproductive capacity throughout their life cycle is essential for optimizing biomass production. This study investigates the influence of ultrasonic waves on the reproductive rate of Botryococcus braunii microalgae. Various ultrasonic parameters, including power (56.2, 86.3, and 132.2 W for 37 kHz; 46.3, 67.2, and 101.2 W for 80 kHz), exposure times (3 and 5 min), and time intervals (2 and 4 days) were examined. The best outcomes were obtained with a 37 kHz ultrasonic frequency, 83.6 W power, 3-min exposure, and a 2-day interval, resulting in a maximum relative specific growth rate (16.67%) and relative biomass productivity (34.62%). Beyond these parameters, adjustments in ultrasonic treatment conditions resulted in decreased growth rates and biomass productivity. It is crucial to consider multiple ultrasonic factors, including frequency, power, exposure time, and time intervals, as each parameter significantly impacts microalgae growth.

References

Blifernez-Klassen, O., Chaudhari, S., Klassen, V., Wördenweber, R., Steffens, T., Cholewa, D., and Kruse, O. (2018). Metabolic survey of Botryococcus braunii: Impact of the physiological state on product formation. PLOS ONE, 13(6): e0198976. https://doi.org/10.1371/journal.pone.0198976

Chen, J.T., Mustafa, E.M., Vello, V., Lim, P., Sulaiman, NMN., Majid, N.A., Phang, S., Tahir, P.Md., and Liew, K. (2016). Preliminary assessment of malaysian micro-algae strains for the production of bio jet fuel. IOP Conference Series: Materials Science and Engineering, 152:012042. https://doi.org/10.1088/1757-899X/152/1/012042

Dayananda, C., Sarada, R., Usha Rani, M., Shamala, T., and Ravishankar, G. (2007). Autotrophic cultivation of Botryococcus braunii for the production of hydrocarbons and exopolysaccharides in various media. Biomass and Bioenergy, 31(1):87-93. https://doi.org/10.1016/j.biombioe.2006.05.001

Duan, Z., Tan, X., Dai, K., Gu, H., and Yang, H. (2017). Evaluation on H2O2-aided ultrasonic pretreatment for cell disruption of Chlorella pyrenoidosa. Asia-Pacific Journal of Chemical Engineering, 12(3):502-510. https://doi.org/10.1002/apj.2093

Fu, L., Li, Q., Yan, G., Zhou, D., and Crittenden, J. C. (2019). Hormesis effects of phosphorus on the viability of Chlorella regularis cells under nitrogen limitation. Biotechnology for Biofuels, 12:121. https://doi.org/10.1186/s13068-019-1458-z

Gani, P., Mohamed sunar, N., Peralta, H.M., Abdul Latiff, A., Ab. Razak, A. R. (2016). Influence of initial cell concentrations on the growth rate and biomass productivity of microalgae in domestic wastewater. Applied Ecology and Environmental Research, 14, 399-409. https://doi.org/10.15666/aeer/1402_399409

Gogate, P.R. (2002). Cavitation: an auxiliary technique in wastewater treatment schemes. Advances in Environmental Research, 6:335-358. https://doi.org/10.1016/S1093-0191(01)00067-3

Han, F., Pei, H., Hu, W., Jiang, L., Cheng, J., and Zhang, L. (2016). Beneficial changes in biomass and lipid of microalgae Anabaena variabilis facing the ultrasonic stress environment. Bioresource Technology, 209:16-22. https://doi.org/10.1016/j.biortech.2016.02.103

Hanief, S., Prasakti, L., Pradana, Y.S., Cahyono, R.B., and Budiman, A. (2020). Growth kinetic of Botryococcus braunii microalgae using logistic and gompertz models. International conference on science and applied science; July 7, 2020; Surakarta, Indonesia; AIP Conference Proceedings, p. 2296. https://doi.org/10.1063/5.0030459

Joyce, E., King, P., and Mason T. (2014). The effect of ultrasound on the growth and viability of microalgae cells. Journal of Applied Phycology, 26:1741-1748. https://doi.org/10.1007/s10811-013-0202-5

Liu Y., Liu X., Cui Y., Yuan W. (2022), Ultrasound for microalgal cell disruption and product extraction: A review. Ultrasonics Sonochemistry, 87:106054. https://doi.org/10.1016/j.ultsonch.2022.106054

Onyeaka, H., Miri, T., Obileke, K., Hart, A., Anumudu, C., and Al-Sharify, Z. T. (2021). Minimizing carbon footprint via microalgae as a biological capture. Carbon Capture Science Technology, 1:100007. https://doi.org/10.1016/j.ccst.2021.100007

Pereira, R.N., Jaeschke, D.P., Mercali, G.D., Rech, R., and Marczak, L.D.F. (2023). Impact of ultrasound and electric fields on microalgae growth: a comprehensive review. Brazilian Journal of Chemical Engineering, 40:1-16. https://doi.org/10.1007/s43153-022-00281-z

Saatovich, S.Z., Mamatkulovich, K.I., and Nortoji, K. (2021). Stress factors’ effects on the induction of lipid synthesis of microalgae. Journal of Applied Biology and Biotechnology, 9(6):149-153. https://doi.org/10.7324/JABB.2021.96019-1

Shi-Kai, W., Feng, W., Amanda, R.S., Chen, G., and Chun-Zhao, L. (2014). Botryococcus braunii cells: Ultrasound-intensified outdoor cultivation integrated with in situ magnetic separation. Bioresource Technology, 167:376-382. https://doi.org/10.1016/j.biortech.2014.06.028

Sivaramakrishnan, R. and Incharoensakdi, A. (2019). Low power ultrasound treatment for the enhanced production of microalgae biomass and lipid content. Biocatalysis and Agricultural Biotechnology, 20:101230. https://doi.org/10.1016/j.bcab.2019.101230

Vintila, A.C.N., Vinatoru, M., Galan, A.M., Vlaicu, A., Ciltea-Udrescu, M., Paulenco, A., and Calinescu, I. (2023). The influence of ultrasound on the growth of Nannochloris sp. in modified growth medium. Life, 13(2):413. https://doi.org/10.3390/life13020413

Wang, B., Li, Y., Wu, N., and Lan, C.Q. (2008). CO2 bio-mitigation using microalgae. Applied Microbiology and Biotechnology, 79:707-718. https://doi.org/10.1007/s00253-008-1518-y

Wang, M. and Yuan W. (2016). Modeling bubble dynamics and radical kinetics in ultrasound induced microalgal cell disruption. Ultrasonics Sonochemistry, 28:7-14. https://doi.org/10.1016/j.ultsonch.2015.06.025

Wu, X., and Mason, T. (2017). Evaluation of power ultrasonic effects on algae cells at a small pilot scale. Water, 9(7):470. https://doi.org/10.3390/w9070470

Wu, X., Joyce, E.M., and Mason, T.J. (2012). Evaluation of the mechanisms of the effect of ultrasound on Microcystis aeruginosa at different ultrasonic frequencies. Water Research, 46:2851-2858. https://doi.org/10.1016/j.watres.2012.02.019

Xiao, S. and Ju, L. (2018). Energy-efficient ultrasonic release of bacteria and particulates to facilitate ingestion by phagotrophic algae for waste sludge treatment and algal biomass and lipid production. Chemosphere, 209:588-598. https://doi.org/10.1016/j.chemosphere.2018.06.120

Xiao-Man, S., Lu-Jing, R., Quan-Yu, Z., Xiao-Jun, J., and He, H. (2018). Microalgae for the production of lipid and carotenoids: a review with focus on stress regulation and adaptation. Biotechnology for Biofuels, 11(1):272. https://doi.org/10.1186/s13068-018-1275-9

Xu, L., Wang, S., Wang, F., Guo, C., and Liu, C.-Z. (2014). Improved biomass and hydrocarbon productivity of Botryococcus braunii by periodic ultrasound stimulation. BioEnergy Research, 7:986-992. https://doi.org/10.1007/s12155-014-9441-9

Yamamoto, K., King, P., Wu, X., Mason, T., and Joyce, E. (2015). Effect of ultrasonic frequency and power on the disruption of algal cells. Ultrasonics sonochemistry, 24:165-171. https://doi.org/10.1016/j.ultsonch.2014.11.002

Zhang, G., Zhang, P., Wang, B., and Liu, H. (2006). Ultrasonic frequency effects on the removal of Microcystis aeruginosa. Ultrasonics Sonochemistry, 13:446-450. https://doi.org/10.1016/j.ultsonch.2005.09.012

Zhang, L., Li, B., Wu, Z., Gu, L., and Yang, Z. (2016). Changes in growth and photosynthesis of Mixotrophic Ochromonas sp. in response to different concentrations of glucose. Journal of Applied Phycology, 28:2671-2678. https://doi.org/10.1007/s10811-016-0832-5

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Published

2024-04-19

How to Cite

Salaeh, A. (2024). INCREASING GROWTH RATE OF Botryocuccus braunii USING ULTRASONIC WAVES. Suranaree Journal of Science and Technology, 31(1), 030172(1–8). https://doi.org/10.55766/sujst-2024-01-e02492

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