CHANGES IN THE PROFILE OF VOLATILE COMPOUND IN SEED STORAGE OF RUBUS FRAXINIFOLIUS
DOI:
https://doi.org/10.55766/sujst-2023-06-e02235Keywords:
GC-MS, Metabolite, Raspberry seed, Rubus, SeedAbstract
Storage conditions are one of the factors that affect seed quality. However, storage cannot improve seed quality, but it can maintain seeds in good condition, including their volatile compounds. Therefore, the study aimed to determine the changes of volatile compounds during seed storage on Rubus fraxinifolius seeds. The seeds were obtained from Cibodas Botanical Garden and divided into three groups i.e., seed without storage, seed stored at room temperature for four months, and seed stored in the refrigerator for four months. The seed extract using ethanol and methanol solvents was analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). The results showed that the total volatile compounds detected in the ethanol and methanol extracts of R. fraxinifolius seed were 113 and 80, respectively. Seven types of main volatile compounds are found in all seed treatments and solvents included g-Sitosterol, g-Tocopherol, 9-Octadecenamide, (Z)-, 9,12-Octadecadienoic acid (Z,Z)-, 9,19-Cyclolanostan-3-ol, 24-methylene-,(3.beta.)-, dl-a-Tocopherol, and Squalene. Compounds were belong to sterols, vitamin E, fatty acids, and triterpenoids. In addition, seed storage treatment increased the percentage of specific volatile compounds, such as g-Sitosterol, g-Tocopherol, 2,4-Decadienal, (E,E)-, 2,4-Decadienal, (E,Z)-, 9-Octadecenamide, (Z)-, 9,12-Octadecadienoic acid (Z,Z)-, 9,19-Cyclolanostan-3-ol,24-methylene-, (3.beta.)-, dl-a-Tocopherol, Eicosane, n-Hexadecanoic acid, and Squalene.
References
Aguirre, M., Kiegle, E., Leo, G., and Ezquer, I. (2018). Carbohydrate reserves and seed development: An overview. Plant Reproduction, 31:263-290.
Ali, V., Rashid, A., Kumar, D., and Vyas, D. (2023). Stage-specific metabolomics suggests a trade-off between primary and secondary metabolites for nutritional advantage in Lepidium latifolium L. Food Chemistry, 419:136035. Doi: https://doi.org/https://doi.org/10.1016/j. foodchem.2023.136035
Ameachi, N.C. and Chijioke, C.L. (2018). Evaluation of bioactive compounds in Pseudarenthemum tunicatum leaves using gas chromathography-mass spectrometry. Scientific Papers-Series Management Economic Engineering in Agriculture and Rural Development, 18(1):53-59.
Ash, M.M., Wolford, K.A., Carden, T.J., Hwang, K.T., and Carr, T.P. (2011). Unrefined and refined black raspberry seed oils significantly lower triglycerides and moderately affect cholesterol metabolism in male Syrian hamsters. Journal of Medicinal Food, 14(9):1,032-1,038.
Bakar, M.F.A., Ismail, N.A., Isha, A., and Ling, A.L.M. (2016). Phytochemical composition and biological activities of selected wild berries (Rubus moluccanus L., R. fraxinifolius Poir., and R. alpestris Blume). Evidence-based Complementary and Alternative Medicine: Ecam, 2016:1-10.
Dalawai, D. and Murthy, H.N. (2021). Chemical profile and antioxidant properties of Andrographis producta (C.B. Clarke) Gamble. Pharmacognosy Journal, 13(2):475-485. Doi: https://doi.org/10.5530/pj.2021.13.60
Desmiaty, Y. and Elya, B. (2021). Unripe fruit of Rubus fraxinifolius as a potential source of antioxidant and antielastase agent. International Journal of Applied Pharmaceutics, 13(2):78-81.
Desmiaty, Y., Elya, B., Saputri, F.C., Hanafi, M., and Prastiwi, R. (2018). Antioxidant activity of Rubus fraxinifolius Poir. and Rubus rosifolius J. Sm. leaves. Journal of Young Pharmacists, 10 (2s):S93.
Desmiaty, Y., Hanafi, M., Saputri, F.C., Elya, B., Rifai, E.A., and Syahdi, R.R. (2021). Two triterpenoids from Rubus fraxinifolius leaves and their tyrosinase and elastase inhibitory activities. Scientific reports, 11(1):20452.
Dewi, R.T., Fitria, I., Sundowo, A., Agustian, E., Ismaini, L., Normasiwi, S., Noviady, I., Destri, and Surya, M.I. (2019). Phytochemical Constituent’s Comparison Using Various Drying Effects on Rubus Fraxinifolius Pour Leaves. Current Agriculture Research Journal, 7(3):310-317. Doi: https://doi.org/10.12944/CARJ.7.3.06
Famiani, F., and Walker, R.P. (2009). Changes in abundance of enzymes involved in organic acid, amino acid and sugar metabolism, and photosynthesis during the ripening of blackberry fruit. Journal of the American Society for Horticultural Science, 134(2):167-175.
Fraga-Corral, M., Otero, P., Echave, J., Garcia-Oliveira, P., Carpena, M., Jarboui, A., Nuñez-Estevez, B., Simal-Gandara, J., and Prieto, M.A. (2021). By-products of agri-food industry as tannin-rich sources: A review of tannins’ biological activities and their potential for valorization. Foods, 10(1):137. Doi: https://doi.org/https://doi.org/10.3390/foods10010137
Fujiwara, T., Nambara, E., Yamagishi, K., Goto, D.B., and Naito, S. (2002). Storage proteins. The Arabidopsis Book/American Society of Plant Biologists, 1, American Society of Plant Biologists.
Huang, Z.R., Lin, Y.K., and Fang, J.Y. (2009). Biological and pharmacological activities of squalene and related compounds: Potential uses in cosmetic dermatology. Molecules, 14(1):540-554. Doi: https://doi.org/10.3390/molecules14010540
Jan, R., Asaf, S., Numan, M., Lubna, and Kim, K.-M. (2021). Plant secondary metabolite biosynthesis and transcriptional regulation in response to biotic and abiotic stress conditions. Agronomy, 11(5):968.
Jiang, Q., Im, S., Wagner, J.G., Hernandez, M.L., and Peden, D.B. (2022). Gamma-tocopherol, a major form of vitamin E in diets: Insights into antioxidant and anti-inflammatory effects, mechanisms, and roles in disease management. Free Radical Biology and Medicine, 178(2):347-359. Doi: https://doi.org/10.1016/j.freeradbiomed.2021.12.012
Kaur, M., Tak, Y., Bhatia, S., Asthir, B., Lorenzo, J.M., and Amarowicz, R. (2021). Crosstalk during the carbon-nitrogen cycle that interlinks the biosynthesis, mobilization and accumulation of seed storage reserves. International Journal of Molecular Sciences, 22(21):12,032.
Kolo, E., and Tefa, A. (2016). Pengaruh kondisi simpan terhadap viabilitas dan vigor benih tomat (Lycopersicum esculentum Mill). Savana Cendana, 1(03):112-115. Doi: https://doi.org/10.32938/sc.v1i03.57
Krishnaveni, M., Dhanalakshmi, R., and Nandhini, N. (2014). GC-MS analysis of phytochemicals, fatty acid profile, antimicrobial activity of gossypium seeds. International Journal of Pharmaceutical Sciences Review and Research, 27(1):273-276.
Likittrakulwong, W., Chanburee, S., Kitpot, T., Ninjiaranai, P., and Pongpamorn, P. (2023). Phytochemical properties, in vitro antimicrobial, and bioactive compounds of banana peel extractions using GC-MS. Natural and Life Sciences Communications, 22(2):e2023021. Doi: https://doi.org/10.12982/NLSC.2023.021
Lozano-Grande, M.A., Gorinstein, S., Espitia-Rangel, E., Dávila-Ortiz, G. and Martínez-Ayala, A.L. (2018). Plant sources, extraction methods, and uses of squalene. International Journal of Agronomy, 2018:1-13. Doi: https://doi.org/10.1155/2018/1829160
Monforte, M.T., Smeriglio, A., Germanò, M.P., Pergolizzi, S., Circosta, C., and Galati, E.M. (2018). Evaluation of antioxidant, antiinflammatory, and gastroprotective properties of Rubus fruticosus L. fruit juice. Phytotherapy Research, 32(7):1,404-1,414.
Morales, M.L., Callejón, R.M., Ubeda, C., Guerreiro, A., Gago, C., Miguel, M.G., and Antunes, M.D. (2014). Effect of storage time at low temperature on the volatile compound composition of Sevillana and Maravilla raspberries. Postharvest Biology and Technology, 96:128-134. Doi: https://doi.org/10.1016/j.postharvbio.2014.05.013
Ngo, T. Van, Scarlett, C.J., Bowyer, M.C., Ngo, P.D., and Vuong, Q. Van. (2017). Impact of different extraction solvents on bioactive compounds and antioxidant capacity from the root of Salacia chinensis L. Puértolas, E. (Ed). Journal of Food Quality, 2017, Hindawi., 9305047. Doi: https://doi.org/10.1155/2017/9305047
Oomah, B.D., Ladet, S., Godfrey, D.V, Liang, J., and Girard, B. (2000). Characteristics of raspberry (Rubus idaeus L.) seed oil. Food chemistry, 69(2):187-193.
Rosales-Garcia, T., Jimenez-Martinez, C. and Davila-Ortiz, G. (2017). Squalene extraction: Biological Sources and extraction methods. International Journal of Environment, Agriculture and Biotechnology, 2(4):1,662-1,670. Doi: https://doi.org/10.22161/ijeab/2.4.26
Roush, R.T. (2001). Biotechnology and Weed Management. In: Proceedings of the 10 th Australian Agronomy Conference, Hobart. 2001.
Saleh, B. (2023). GC / MS analysis of hypericum perforatum L. (Hypericaceae) Species. 19(2):25-33.
Sari, M., Tamrin, Kaban, J., and Alfian, Z. (2020). Bioactivity and phytochemical constituents of extract etanol from Musa paradisiaca Linn. (Icocsti 2019), 89-95. Doi: https://doi.org/10.5220/0008855100890095
Shamsudin, N.A., Matawali, A., and Gansau, J.A. (2019). Comparison of antioxidant activity and phytochemical content of Borneo wild berry, Rubus fraxinifolius (Rogimot). Transactions on Science and Technology, 6(1):36-41.
Sundarraj, S., Thangam, R., Sreevani, V., Kaveri, K., Gunasekaran, P., Achiraman, S. and Kannan, S. (2012). γ-Sitosterol from Acacia nilotica L. induces G2/M cell cycle arrest and apoptosis through c-Myc suppression in MCF-7 and A549 cells. Journal of Ethnopharmacology, 141(3):803-809. Doi: https://doi.org/10.1016/j.jep.2012.03.014
Surya, M.I., Suhartati, S., Ismaini, L., Lusini, Y., Destri, D., Anggraeni, D., Normasiwi, S., Asni, N., and Sidiq, M. (2018). Fruit nutrients of five species of wild raspberries (Rubus spp.) from Indonesian Mountain’s Forests. Journal of Tropical Life Science, 8 (1): 75–80. Doi: https://doi.org/10.11594/jtls.08.01.13
Theanphong, O., Jenjittikul, T., and Mingvanish, W. (2016). Essential Oil Compositions from Root and Rhizome of Zingiber niveum Mood Theilade from Laos. Biological and Chemical Research, 2015:157-160.
Tripathi, N., Kumar, S., Singh, R., Singh, C.J., Singh, P., and Varshney, V.K. (2013). Isolation and Identification of γ-Sitosterol by GC-MS from the Leaves of Girardinia heterophylla (Decne). Open Bioactive Compounds Journal, 4 (199):25-27. Doi: https://doi.org/10.2174/1874847301004010025
Weselake, R.J. (2020). Storage lipids. In: Plant Lipids. Blackwell, 162-225.
Yang, L., Wen, K.S., Ruan, X., Zhao, Y.X., Wei, F., and Wang, Q. (2018). Response of plant secondary metabolites to environmental factors. Molecules, 23(4):1-26. Doi: https://doi.org/doi:10.3390/molecules23040762
