DESIGN AND DEVELOPMENT OF 3D ROTATIONAL BIOREACTOR FOR 3D CELL CULTURE

Chakkapong Chamroon; Kittiya Thunsiri; Sarawut Kumphune; Wassanai Wattanutchariya; Kanya Rattanamongkhonkun

doi:10.55766/sujst-2023-02-e01601

Authors

Chakkapong Chamroon Chiang Mai University
Kittiya Thunsiri Chiang Mai University
Sarawut Kumphune Chiang Mai University
Wassanai Wattanutchariya Chiang Mai University
Kanya Rattanamongkhonkun Chiang Mai University

DOI:

https://doi.org/10.55766/sujst-2023-02-e01601

Keywords:

3D cell culture, 3D rotation, Bioreactor, pharmaceutical industry, programable

Abstract

Currently, cell and tissue culture are a key to success for regenerative medicine and the pharmaceutical industry for implementing new therapeutic concepts. Cell culture is a complex process in which cells are grown under controlled conditions. Three-dimensional (3D) scaffolds provide space for cell adhesion and growth in 3D structures. However, in 3D cell culture, the ability to supply nutrients and oxygen, and the simultaneous removal of waste products and metabolites are the limiting factors for cells survival, due to nutrient starvation and metabolite toxicity. Therefore, an in-house 3D rotational bioreactor, for 3D cell culture, was designed, fabricated, and developed based on a chitosan porous scaffold. The 3D rotational bioreactor provides a continuously moving culture medium led to high rates of mass transfer throughout the scaffolds. The rotation speed and duration are programable and provide a continuous free flow during the rotating period. The results demonstrated the potential of an in-house 3D rotational bioreactor for better nutrient supply and waste transfer systems outweighs the conventional cell culture technique.

References

Agueda, J.R.H.S., Chen, Q., Maalihan, R.D., Ren, J., da Silva, Í.G.M., Dugos, N.P., Caldona, E.B., and Advincula, R.C. (2021). 3D printing of biomedically relevant polymer materials and biocompatibility. MRS Communications, 11:197-212. https://doi.org/10.1557/s43579-021-00038-8.

Altmann, B., Grün, C., Nies, C., and Gottwald, E. (2021). Advanced 3D cell culture techniques in micro-bioreactors, Part II: systems and applications. Processes, 9(1):21. https://doi.org/10.3390/pr9010021.

Bang, K.H. and Kim, K.S. (2021). A strategy for improvement of recombinant pharmaceutical industry using animal cell culture technology. Yakhak Hoeji, 65(1):1-9. https://doi.org/10.17480/psk.2021.65.1.1.

Chung, T.W., Yang, J., Akaike, T., Cho, K.Y., Nah, J. W., Kim, S.I., and Cho, C.S. (2002). Preparation of alginate/

galactosylated chitosan scaffold for hepatocyte attachment. Biomaterials, 23(14):2827-2834.

https://doi.org/10.1016/S0142-9612(01)00399-4.

Da Silva, K., Kumar, P., van Vuuren, S.F., Pillay, V., and Choonara, Y.E. (2021). Three-Dimensional Printability of an ECM-Based Gelatin Methacryloyl (GelMA) Biomaterial for Potential Neuroregeneration. ACS Omega, 6(33):21368-21383. https://doi.org/10.1021/acsomega.1c01903.

Dasgupta, S. and Barui, A. (2021). 3D cell culture for pharmaceutical application. In: Advances and Challenges in Pharmaceutical Technology, Nayak, A.K., Pal, K., Banerjee, I., Maji, S., and Nanda, U. (eds). p. 261-282. https://doi.org/10.1016/B978-0-12-820043-8.00003-7.

Jaifu, J., Thunsiri, K., Udomsom, S., Boonyawan, D., and Wattanutchariya, W. (2019). Blood absorption improvement of a naturally derived hemostatic agent by atmospheric pressure plasma jet. Materials Today: Proceedings, 17(4):2088-2096. https://doi.org/10.1016/j.matpr.2019.06.258.

Jensen, C. and Teng, Y. (2020). Is it time to start transitioning from 2D to 3D cell culture?. Frontiers in Molecular Biosciences, 7:33. https://doi.org/10.3389/fmolb.2020.00033.

Kizilova, N. (2021). 3D bioreactors for cell culture: fluid dynamics aspects. In: The International Conference of the Polish Society of Biomechanics, Springer, Cham., p. 80-99. https://doi.org/10.1007/978-3-030-86297-8_8.

Kropp, C., Massai, D., and Zweigerdt, R. (2017). Progress and challenges in large-scale expansion of human pluripotent stem cells. Process Biochemistry, 59(Part B):244-254. https://doi.org/10.1016/j.procbio.2016.09.032.

Li, Z. and Li, X. (2021). Will Innovation of Pharmaceutical Manufacturing Improve Perceived Health?. Frontiers in Public Health, 9:290. https://doi.org/10.3389/fpubh.2021.647357.

Liu, X., Chen, M., Luo, J., Zhao, H., Zhou, X., Gu, Q., Yang, H., Zhu, X., Cui, W., and Shi, Q. (2021). Immunopolarization-regulated 3D printed-electrospun fibrous scaffolds for bone regeneration. Biomaterials, 276:121037. https://doi.org/10.1016/j.biomaterials.2021.121037.

Lv, D., Hu, Z., Lu, L., Lu, H., and Xu, X. (2017). Three dimensional cell culture: A powerful tool in tumor research and drug discovery. Oncology letters, 14(6):6999-7010. https://doi.org/10.3892/ol.2017.7134.

Nikolova, M.P. and Chavali, M.S. (2019). Recent advances in biomaterials for 3D scaffolds: A review. Bioactive materials, 4:271-292. https://doi.org/10.1016/j.bioactmat.2019.10.005.

Nishi, M., Matsumoto, R., Dong, J., and Uemura, T. (2013). Engineered bone tissue associated with vascularization utilizing a rotating wall vessel bioreactor. Journal of Biomedical Materials Research Part A, 101(2):421-427. https://doi.org/10.1002/jbm.a.34340.

Ozturk, S., and Hu, W.S. (Eds.). (2005). Cell Culture Technology For Pharmaceutical and Cell-Based Therapies. CRC Press, 776p. https://doi.org/10.1201/9780849351068.

Radtke, A.L. and Herbst-Kralovetz, M.M. (2012). Culturing and applications of rotating wall vessel bioreactor derived 3D epithelial cell models. Journal of Visualized Experiments, 62:e3868. https://doi.org/10.3791/3868.

Scherer, F.M. (2000). The pharmaceutical industry. Handbook of Health Economics, 1(part B):1297-1336. https://doi.org/10.1016/S1574-0064(00)80038-4.

Sun, M., Liu, A., Yang, X., Gong, J., Yu, M., Yao, X., Wang, H., and He, Y. (2021). 3D cell culture-can it be as popular as 2D cell culture?. Advanced NanoBiomed Research, 1(5):2000066. https://doi.org/10.1002/anbr.202000066.

Thunsiri, K., Oonjai, A., and Wattanutchariya, W. (2018). Characterization of hydroxyapatite/silk fibroin/ chitosan scaffold for cartilage tissue engineering. In: Key Engineering Materials, Trans Tech Publications Ltd., 775:120-126. https://doi.org/10.4028/www.scientific.net/KEM.775.120.

Thunsiri, K., Pitjamit, S., Pothacharoen, P., Pruksakorn, D., Nakkiew, W., and Wattanutchariya, W. (2020). The 3D-printed bilayer's bioactive-biomaterials scaffold for full-thickness articular cartilage defects treatment. Materials, 13(15):3417. https://doi.org/10.3390/ma13153417.

Thunsiri, K., Udomsom, S., and Wattanutchariya, W. (2016). Characteristic of pore structure and cells growth on the various ratio of silk fibroin and hydroxyapatite in Chitosan base scaffold. In: Key Engineering Materials, Trans Tech Publications Ltd. 675:459-462. https://doi.org/10.4028/www.scientific.net/KEM.675-676.459.

Wattanutchariya, W., and Thunsiri, K. (2015). Effects of Fibroin Treatments on Physical and Biological Properties of Chitosan/Hydroxyapatite/Fibroin Bone's Scaffold. In: Applied Mechanics and Materials, 799:488-492. Trans Tech Publications Ltd. https://doi.org/10.4028/www.scientific.net/AMM.799-800.488.

Wattanutchariya, W., Oonjai, A., and Thunsiri, K. (2016). Effects of Hydroxyapatite/Silk Fibroin/Chitosan Ratio on Physical Properties of Scaffold for Tissue Engineering Application. In: Materials Science Forum, Trans Tech Publications Ltd. 872:261-265. https://doi.org/10.4028/www.scientific.net/MSF.872.261.

Wattanutchariya, W., Thunsiri, K., and Puntawang, S. (2020). Efficiency Improvement of Gelatin-Based Wound Dressings by Silk Fibroin and Chitosan. In: Materials Science Forum, Trans Tech Publications Ltd. 995:97-102. https://doi.org/10.4028/www.scientific.net/MSF.995.97.