IN VITRO RESPONSE OF VARIOUS GROWTH REGULATORS ON THE REGENERATION OF BOUGAINVILLEA SPECTABILIS WILLD
Keywords:
Bougainvillea, shoot tips culture, callus induction, in vitro regeneration, rootingAbstract
Bougainvillea is commonly propagated by cuttings but this method is tedious and time consuming with very little success. Tissue culture technique has been proved as a potential tool for quick and mass propagation of several ornamental species. The research was carried out to investigate optimum levels of BAP, glutamine, IAA and IBA supplemented to MS medium for micro propagation of Bougainvillea spectabilis Willd. Shoot apices were excised, sterilized and then cultured on MS medium supplemented with plant regulators for callus induction and plant regeneration. Maximum number of plantlets (81.25%) with conspicuous callus formation was observed with BAP 1.0 mg/l + Glutamine 500 mg/l followed by BAP 1.0 mg/l + 250 mg/l Glutamine where 64.58% plantlets have been developed. The minimum number of plantlets developed on control MS basic medium was 12.5%. Plantlets produced were then re-cultured in full and half strength MS medium supplemented with different concentrations and combinations of IAA and IBA. It was found that half strength MS medium supplemented with IAA 0.5 mg/l and IBA 0.5 mg/l, proved the best medium where 79.16% of plantlet rooted. Higher average number of roots per plant (30.0) was obtained on IBA 5 mg/l. The lowest number of plantlets (25.0%) rooted when IAA 5.0 mg/l was used only. The lowest average number (2.7) of roots per plant was obtained on MS medium with IAA 2.5 mg/l. The maximum number of plantlets (58.33%) rooted when full strength MS medium was supplemented with higher concentrations (5.0 mg/l) each of IAA and IBA. The maximum number of average roots per plant (15.4) were obtained when full strength MS medium was supplemented with IAA 2.5 mg/l. Half strength MS medium supplemented with IAA and IBA 0.5 mg/l each gave maximum root induction while full strength MS medium with IAA and IBA 5.0 mg/l each proved best for root initiation.
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