EFFECT OF NUTRIENT CONCENTRATION ON THE GROWTH AND LIPID CONTENT OF CHLORELLA VULGARIS CULTIVATION USING FROZEN SEAFOOD EFFLUENT
Keywords:
Nutrient, Chlorella vulgaris, seafood effluent, lipid contentAbstract
Microalgae can offer several benefits for wastewater treatment with their ability to produce amounts of lipid contents for biofuel production. The aim of this research was to evaluate the feasibility of microalgae cultivation using treated saline wastewater from frozen seafood factory. The effect of nutrient concentration on lipid content of Chlorella vulgaris was investigated. The frozen seafood effluent as a saline wastewater used for cultivating microalgae because it contains high nutrients for microalgae growth. Microalgae cultivation can provide several advantages consists of high photosynthesis, growth rate and lipid content. Furthermore, the microalgae are able to use carbon dioxide and produce the renewable energy such as fuels and chemicals. These productions can decrease the using of fossil fuel and greenhouse gas emission. The frozen seafood effluent was mixed with tap water in the ratio of effluent: tap water 10:0, 9:1, 8:2, 7:3, 6:4 and 5:5 by volume that cultivates in R1, R2, R3, R4, R5 and R6, respectively to decrease the concentrations of TKN and TP from the effluent. After microalgae cultivation, the results show that the maximum dry cell weight (DCW) of R1, R2, R3, R4, R5 and R6 was 1.265, 2.529, 1.859, 1.917, 2.729 and 2.533 g/L, respectively. The lipid content of Chlorella vulgaris in R1, R2, R3, R4, R5 and R6 was 3.82±0.06%, 4.58±0.01%, 5.09±0.04%, 4.30±0.02%, 3.70±0.08% and 5.20±0.03%, respectively at the end of cultivation. This study found that nutrient concentration, especially TKN and TP, had more of an effect on the metabolisms of Chlorella vulgaris has been described. In this study, the lipid content of Chlorella vulgaris revealed good increasing trends in the low nutrient concentration from R1 to R3 and the highest lipid content of Chlorella vulgaris represented in R6, which was cultivated in the lowest nutrient concentration (58.1±9.3 mg/L of TKN and 10.1±0.9 mg/L of TP). The low nutrient condition can induce high lipid and biomass content of Chlorella vulgaris.
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