THE DEVELOPMENT OF AN ALTERNATIVE ALGAL LIPID QUANTIFICATION METHOD BASED ON RESISTANCE OF SCREEN PRINTED CARBON PAPER ELECTRODES
Keywords:
Algal lipid, biodiesel, lipid quantification, screen-printed carbon electrodesAbstract
Quantification of algal lipids may require large sample sizes and specialized equipment. Here we present an alternative principle using screen-printed carbon electrodes. Based on a set of experiments using soy bean oil, glyceryl trioleate and crude lipids extracted from Spirulina (Arthrospira platensis), Ankistrodesmus sp. and Chlorella vulgaris, the normalized resistance of the electrodes changes with concentration of the lipid deposited. The lipid concentration- normalized resistance relationships are of bell patterns with a linear range started from concentration = 0-20 mg/ml and ended around 30-100 mg/ml, depending on lipid types. The sensitivity of the measurement is improved by about an order when fatty acid methyl esters obtained from Ankistrodesmus was used instead of the crude extract of the same algae. The technique consumes a dry algal biomass of 0.1-4 mg for an analysis. The amount is much less than that conventionally required for the gravitational analysis (80-100 mg) and smaller than that demanded in the sample preparation for analysis with GC (7-10 mg). The technique has a potential to develop into a cost-effective and less equipment-dependent lipid quantification for microfarms.
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