THERMOTOLERANT -GALACTOSIDASE PRODUCING BACTERIA APPLIED FOR FERMENTATION OF SOYBEAN MEAL IN ANIMAL FEED
Keywords:
-Galactosidase, animal feed, raffinose family oligosaccharides, soybean mealAbstract
Raffinose family oligosaccharides (RFOs) are one of anti-nutritional factors (ANFs) found in soybean meal (SBM) and unable to hydrolyze by enzymes in intestinal tract of monogastric animals resulted in flatulence which reduced their diet performance. Removal these oligosaccharides from SBM by -galactosidase is therefore an effective approach for monogastric animal feeds. The aim of this research was to isolate and screen thermotolerant bacteria capable to digest RFOs in SBM. Only 11 out of 28 thermotolerant isolates appeared light blue colony on selective medium. The isolates showed the -galactosidase activity in range of 0.100-0.600 U/ml. By 16S rDNA sequence analysis, the isolate S2 having enzyme activity at 0.417 U/ml was identified as Bacillus megaterium which is one of bacteria in the list of animal feed supplement. The α-galactosidase production of that S2 isolate was maximal reached at 32 h after cultivating in LB supplemented 1% raffinose. Increasing of B. megaterium S2 growth on SBM from 7.47 to 9.46 log CFU/g indicated its ability to use SBM as sole nutrient source. Moreover, it was also able to degrade 87.21% raffinose and 97.46% stachyose in autoclaved SBM within 72 h of fermentation which indirectly suggested the removal of flatulence-causing oligosaccharides by its -galactosidase activity. Therefore, B. megaterium S2 could be potentially applied for production of fermented SBM containing low RFOs further used as animal feed supplement.
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