TOXICOLOGICAL AND HISTOPATHOLOGICAL EFFECTS OF Plumbago indica ROOT EXTRACT IN HAMSTERS
Keywords:
Plumbago indica, toxicity, histopathology, biochemical testAbstract
The Plumbago indica roots have been used as traditional medicines against many maladies; however, the information of toxicity has been needed when consumed in the high volume or for a long period. The objective of this study was to investigate the toxicity of crude extract from P. indica root (cPI) in hamsters by mortality, behavior changes, histochemical and histopathological changes. Golden Syrian hamsters were randomly divided into 5 groups and administered with cPI at 0, 100, 400, 1,000, and 3,000 mg/kg orally for 24 h and every day for 7 days for studying acute and subacute toxicity assessments, respectively. Neither mortality nor behavior changes of animals were found during acute and subacute toxicity tests. Weights of liver and kidney in cPI treated at 3,000 mg/kg were significantly increased after oral administration at 24 h and 7 days. The biochemical parameters revealed insignificant differences in the liver function tests (ALT and AST) and kidney function tests (creatinine and BUN). The morphological hepatic lobules in the subacute assessment were better than acute assessment. Although glomeruli swelling and angiectasis in the kidney were found in cPI treated group at 3,000 mg/kg for both the 24 h and 7 days, the renal tubular compartments had become similar to the control groups. The LD50 of cPI is classified as category 5 (2,000-5,000 mg/kg). These results suggest that the precaution of users should be concern harmful to hepatotoxic property and renal function in early acute and subacute periods at the high dose of cPI extract.
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