HUMAN ORAL LACTOBACILLI CELL FREE SUPERNATANT FOR COSMETIC CREAM
Keywords:
Antimicrobial, antioxidant, cell free supernatant, cosmetic, lactobacilli, liposome creamAbstract
The Lactobacillus paracasei SD1 and Lactobacillus rhamnosus SD11 are commensal microbes found in the oral cavity of caries-free human. They are widely used in oral health care, by incorporating them into fermented milk or fruit juice, to prevent dental caries and to promote the homeostasis of oral microbiota. However, the cell free supernatant (CFS) of Lactobacilli, which consists of various beneficial substances for dermal health, is wasted as a by-product. Therefore, it was interesting to investigate the activities and the usefulness of CFS of the L. paracasei SD1 and L. rhamnosus SD11 for cosmetic applications. Liposomal technology was used to improve the appearance and skin permeation of CFS. The optimal CFS liposome cream containing 2.5% w/w CFS showed good stability and high antioxidant activity. Moreover, the antimicrobial activity of the CFS liposome cream was the same as commercial products. In addition to that, the result of in vitro release study demonstrated that the CFS liposome cream could maintain and control the release of active compounds over 24 h. Thus, this study highlights the advantages of the CFS of L. paracasei SD1 and L. rhamnosus SD11 for dermal applications and not wasting them as by-products. The study also suggested that the CFS of L. paracasei SD1 and L. rhamnosus SD11 could be good bio-natural sources for cosmetic ingredients, and the CFS liposome cream could be promising for use as a cosmetic product.
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