PREVALENCE AND VIRULENCE GENE OF Salmonella STRAINS ISOLATED FROM DUCKS IN NORTH VIETNAM

Dang  Xuan Binh; Dang  Thi Mai Lan; Pham  Thi Phuong Lan; Nghiem  Ngoc Minh

Authors

Dang Xuan Binh Thai Nguyen University of Agriculture and Forestry: Quyet Thang Commune, Thai Nguyen City, Vietnam.
Dang Thi Mai Lan Thai Nguyen University of Agriculture and Forestry: Quyet Thang Commune, Thai Nguyen City, Vietnam.
Pham Thi Phuong Lan Thai Nguyen University of Agriculture and Forestry: Quyet Thang Commune, Thai Nguyen City, Vietnam.
Nghiem Ngoc Minh Institute of Genome Research, Vietnam Academy of Science and Technology: 18 Hoang Quoc Viet, Cau Giay District, Hanoi, Vietnam.

Keywords:

Duck, Salmonella, virulence gene, antibiotic

Abstract

In order to determine the prevalence of Salmonella infection and Salmonellosis on ducks, feces and cloacal swap samples were collected from 3,700 ducks in Thai Nguyen, Bac Giang, Bac Ninh, Lang Son and Tuyen Quang provinces in 2016 and 2018. The results show that: Diseases caused by Salmonella in ducks were found in 100% of the farms and 11.51% of the flocks. Of all Salmonella bacteria studied, 6.63% belonged to serotype Salmonella enteritidis, 14.79% were Salmonella typhimurium, 11.73% were Salmonella anatum, 7.65% were Salmonella montevideo, 10.20% were Salmonella heidelberg, 8.67% were Salmonella indiana, 7.65% were Salmonella orion, 10.20% were Salmonella senftenberg, and Salmonella wetevreden accounted for 7.65%. DNA encoded genes enterotoxin (Stn), adherence (fimA), and invasion (InvA) were identified; Salmonella enteritidis, 92.30%, 69.23%, and 53.84%; Salmonella typhimurium, 89.65%, 72.41%, and 65.51%; Salmonella anatum, 82.60%, 69.56%, and 56.52%; Salmonella montevideo, 86.66%, 60.00%, and 46.66%; Salmonella indiana, 88.23%, 58.82%, and 35.29%; Salmonella orion, 80.00%, 53.33%, and 33.33%; Salmonella senftenberg, 95.00%, 60.00%, and 45.00%; and Salmonella wetevreden: 86.66%, 40.00%, and 26.66% respectively. Salmonella isolates were susceptible to nalidixic acid, ceftazidime, ciprofloxacin, rifampicin, spectinomycin, trimethoprim-sulfamethoxazole, and nitrofurantoin. Of which, the antibiotics with the strongest antimicrobial resistance from Salmonella isolates were kanamycin, colistin, and neomycin.

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