ZNO AND TIO2 NANOPARTICLES STIMULATE CALLUS INDUCTION AND SECONDARY METABOLITE PRODUCTION IN Indica RICE
Keywords:
Callus induction, Flavonoids, Indica rice, Phenolic compounds, TiO2 nanoparticles\, ZnO nanoparticlesAbstract
Metal nanoparticles are widely used in various fields of industry and their impact in the agricultural field is increasing. This research was carried out to investigate the effects of ZnO and TiO2 nanoparticles on callus induction and plant metabolism in indica rice. Indica rice was assessed in plant tissue culture condition to estimate the influence of metal nanoparticles on morphological and physiological factors over 4 weeks. The seeds of Oryza sativa L. var. KDML105 were exposed to 1-3 mg L-1 of 2,4-dichlorophenoxyacetic acid (2,4-D) in the callus induction medium. The induced-calli were cultured on the callus induction medium supplemented with 2 mg L-1 of 2,4-D and showed a high increase in size and dry weight contents under the light condition when compared with the control and other treatments. Moreover, this research also investigated the potential impacts of ZnO and TiO2 nanoparticles at 200, 400 and 800 mg L-1 on morphological and metabolic responses of the induced-calli cultured on the callus induction medium supplemented with 2,4-D and each type of metal nanoparticles. At 4 weeks after treatment, the induced-calli were analyzed for plant growth and assays on aspects of their metabolism were undertaken. The induced-calli exposed to 200 mg L-1 of ZnO or TiO2 nanoparticles were found to have significant increase of biomass (size and dry weight of callus), total phenolic compounds and total flavonoid contents in KDML105 variety. The results demonstrated that utilization of ZnO and TiO2 nanoparticles at appropriate concentration could positively induce the production of beneficial secondary metabolites in the induced-calli of indica rice and are worthy of further study for applications in other plant species.
References
Adhikari, T., Kundu, S., Biswas, A., Tarafdar, J., and Subba Rao, A. (2015). Characterization of zinc oxide nano particles and their effect on growth of maize (Zea mays L.) plant. J. Plant Nutr., 38:1505–1515.
Alothman, M., Bhat, R., and Karim, A.A. (2009). Antioxidant capacity and phenolic content of selected tropical fruits from Malaysia, extracted with different solvents. Food Chem., 115:785–788.
Bakhshandeh, E., Rahimian, H., Pirdashti, H., and Nematzadeh, G.A. (2015). Evaluation of phosphate-solubilizing bacteria on the growth and grain yield of rice (Oryza sativa L.) cropped in northern Iran. J. Appl. Microbiol., 119:1371–1382.
Balbuena, T.S., Santa-Catarina, C., Silveira, V., Kato, M.J., and Floh, E.I. (2009). In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae). Acta Bot. Bras., 23:274–281.
Bicer, P.O., Demirci, T., Aşcı, O.A., and Baydar, N.G. (2017). Effects of methyl jasmonate and caffeic Acid applications on secondary metabolite production in madder (Rubia tinctorum) root cultures. Indian J. Pharm. Educ. Res., 51:508–512.
Chatterjee, S.S. (2015). From covalent bonds to eco-physiological pharmacology of secondary plant metabolites. Biochem. Pharmacol., 98:269–277.
Chutipaijit, S. (2015). Establishment of condition and nanoparticle factors influencing plant regeneration from aromatic rice (Oryza sativa). Int. J. Agric. Biol., 17:1049–1054.
Chutipaijit, S., and Sutjaritvorakul, T. (2017). Application of nanomaterials in plant regeneration of rice (Oryza sativa L.). Mater. Today Proc., 4:6140–6145.
Chutipaijit, S., and Sutjaritvorakul, T. (2018). Application of activated charcoal and nanocarbon to callus induction and plant regeneration in aromatic rice (Oryza sativa L.). Chem. Spec. Bioavailab., 30:1–8.
Conway, J.R., Beaulieu, A.L., Beaulieu, N.L., Mazer, S.J., and Keller, A.A. (2015). Environmental stresses increase photosynthetic disruption by metal oxide nanomaterials in a soil-grown plant. ACS Nano, 9:1137–1149.
Feizi, H., Moghaddam, P.R., Shahtahmassebi, N., and Fotovat, A. (2012) Impact of bulk and nanosized titanium dioxide (TiO2) on wheat seed germination and seedling growth. Biol. Trace Elem. Res., 146:101–106.
Frank, T., Röhlig, R.M., Davies, H.V., Barros, E., and Engel, K.H. (2012). Metabolite profiling of maize kernelsdgenetic modification versus environmental influence. J. Agric. Food Chem., 60:3005–3012.
Gardea-Torresdey, J.L., Rico, C.M., and White, J.C. (2014). Trophic transfer, transformation, and impact of engineered nanomaterials in terrestrial environments. Environ. Sci. Technol., 48:2526–2540.
Ghabooli, M., Hosseini Salekdeh, G., and Sepehri, M. (2015). The effect of mycorrhiza-like fungus Piriformospora indica on some morphophysiological traits of rice under normal and drought stress conditions. Plant Prod. Technol., 7:59–69.
Ghorbanpour, M. (2015). Major essential oil constituents, total phenolics and flavonoids content and antioxidant activity of Salvia officinalis plant in response to nano-titanium dioxide. Ind. J. Plant Physiol., 20:249–256.
Ghorbanpour, M., and Hadian, J. (2015). Multi-walled carbon nanotubes stimulate callus induction, secondary metabolites biosynthesis and antioxidant capacity in medicinal plant Satureja khuzestanica grown in vitro. Carbon, 94:749–759.
Gill, S.S., and Tuteja, N. (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol. Biochem., 48:909–930.
Helaly, M.N., El-Metwally, M.A., El-Hoseiny, H., Omar, S.A., and El-Sheery, N.I. (2014). Effect of nanoparticles on biological contamination of in vitro cultures and organogenic regeneration of banana. Aust. J. Crop. Sci., 8:612–624.
Khot, L.R., Sankaran, S.R., Mari, M.J., Ehsani, R., and Schuster, E.W. (2012). Application of nanomaterials in agricultural production and crop protection: a review. Crop Prot., 35:64–70.
Kim, S., Lee, S., and Lee, I. (2012). Alteration of phytotoxicity and oxidant stress potential by metal oxide nanoparticles in Cucumis sativus. Water Air Soil Pollut., 223:2799–2806.
Klaine, S.J., Alvarez, P.J., Batley, G.E., Fernandes, T.F., Handy, R.D., Lyon, D.Y., Mahendra, S., Mclaughlin, M.J., and Lead, J.R. (2008). Nanomaterials in the environment: behavior, fate, bioavailability, and effects. Environ. Toxicol. Chem., 27:1825–1851.
Lee, S., and Huang, W. (2014). Osmotic stress stimulates shoot organogenesis in callus of rice (Oryza sativa L.) via auxin signaling and carbohydrate metabolism regulation. Plant Growth Regul., 73:193–204.
Mirzajani, F., Askari, H., Hamzelou, S., Schober, Y., Rompp, A., Ghassempour, A., and Spengler, B. (2014). Proteomics study of silver nanoparticles toxicity on Oryza sativa L. Ecotoxicol. Environ. Saf., 108:335–339.
Mousavi Kouhi, S.M., Lahouti, M., Ganjeali, A., and Entezari, M.H. (2014). Comparative phytotoxicity of ZnO nanoparticles, ZnO microparticles, and Zn2+ on rapeseed (Brassica napus L.): investigating a wide range of concentrations. Toxicol. Environ. Chem., 96:861–868.
Oloumi, H., Soltaninejad, R., and Baghizadeh, A. (2015). The comparative effects of nano and bulk size particles of CuO and ZnO on glycyrrhizin and phenolic compounds contents in Glycyrrhiza glabra L. seedlings. Ind. J. Plant Physiol., 20:157–161.
Pedro, A.C., Granato, D., and Rosso, N.D. (2016). Extraction of anthocyanins and polyphenols from black rice (Oryza sativa L.) by modeling and assessing their reversibility and stability. Food Chem., 191:12–20.
Qi, M., Liu, Y., and Li, T. (2013). Nano-TiO2 improve the photosynthesis of tomato leaves under mild heat stress. Biol. Trace Elem. Res., 156:323–328.
Ramakrishna, A., and Ravishankae, G.A. (2011). Influence of abiotic stress signals on secondary metabolites in plants. Plant Signal. Behav., 6:1720–1731.
Rico, C.M., Morales, M.I., Mccreary, R., Castillo-Michel, H., Barrios, A.C., Hong, J., Tafoya, A., Lee, W.Y., Varela-Ramirez, A., Peralta-Videa, J.R., and Gardea-Torresdey, J.L. (2013). Cerium oxide nanoparticles modify the antioxidative stress enzyme activities and macromolecule composition in rice seedlings. Environ. Sci. Technol., 47:14110–14118.
Samart, S., Phakamas, N., and Chutipaijit, S. (2018). Assessment of antioxidant enzymes in response to exogenous titanium dioxide (TiO2) nanoparticles in Chainat 1 rice cultivar. Mater. Today Proc., 5:14160–14165.
Scalabrin, E., Radaelli, M., and Capodaglio, G. (2016). Simultaneous determination of shikimic acid, salicylic acid and jasmonic acid in wild and transgenic Nicotiana langsdorffii plants exposed to abiotic stresses. Plant Physiol. Biochem., 103:53–60.
Servin, A.D., Castillo-Michel, H., Hernandez-Viezcas, J.A., Diaz, B.C., Peralta-Videa, J.R., and Gardea-Torresdey, J.L. (2012). Synchrotron micro-XRF and micro-XANES confirmation of the uptake and translocation of TiO2 nanoparticles in cucumber (Cucumis sativus) plants. Environ. Sci. Technol., 46:7637–7643.
Shahsavari, E., Maheran, A.A., Siti Nor Akmar, A., and Hanafi, M.M. (2010). The effect of plant growth regulators on optimization of tissue culture system in Malaysian upland rice. Afr. J. Biotechnol., 9:2089–2094.
Song, U., Jun, H., Waldman, B., Roh, J., Kim, Y., Yi, J., and Lee, E. (2013). Functional analyses of nanoparticle toxicity: a comparative study of the effects of TiO2 and Ag on tomatoes (Lycopersicon esculentum). Ecotoxicol. Environ. Saf., 93:60–67.
Verma, D., Joshi, R., Shukla, A., and Kumar, P. (2011). Protocol for in vitro somatic embryogenesis and regeneration of rice (Oryza sativa L.). Ind. J. Exp. Biol., 49:958–963.
Wang, F.Y., Liu, X.Q., Shi, Z.Y., Tong, R.J., Adams, C.A., and Shi, X.J. (2016). Arbuscular mycorrhizae alleviate negative effects of zinc oxide nanoparticle and zinc accumulation in maize plants - a soil microcosm experiment. Chemosphere, 147:88–97.
Wu, B., Zhu, L., and Le, X.C. (2017). Metabolomics analysis of TiO2 nanoparticles induced toxicological effects on rice (Oryza sativa L.). Environ. Pollut., 230:302–310.
Zhao, L., Huang, Y., Hu, J., Zhou, H., Adeleye, A.S., and Keller, A.A. (2016). 1H NMR and GC-MS based metabolomics reveal defense and detoxification mechanism of cucumber plant under nano-Cu stress. Environ. Sci. Technol., 50:2000–2010.
Zhao, W., Zheng, S., and Ling, H.Q. (2011). An efficient regeneration system and Agrobacterium-mediated transformation of Chinese upland rice cultivar Handao297. Plant Cell Tiss. Org., 106: 475–483
