RUMINAL FATTY ACID BIO-HYDROGENATION IN RESPONSE TO LINSEED OIL AND SUNFLOWER OIL IN COMBINATION WITH FISH OIL ADDITION TO FISTULATED CATTLE’S DIETS
Keywords:
Trans-vaccenic acid, bio–hydrogenation, oil supplementation, fatty acidsAbstract
The effects of linseed oil or sunflower oil in combination with fish oil on ruminal bio-hydrogenation and rumen fermentation in fistulated cattle were studied. Four fistulated cattle were used in a 4×4 Latin square design; the experiment consisted of 4 periods of 21 d in each period, with the first 14 d for adaptation followed by 7 d of measurement period. The animals were fed 3 kg/d of 14% crude protein concentrate and 2 kg/d rice straw. The treatments were: 1) control no oil added, 2) supplemented with 70 g/d linseed oil (LO) plus 70 g/d fish oil (FO), 3) supplemented with 70 g/d sunflower oil (SO) plus 70 g/d FO, and 4) supplemented with 46.7 g/d LO, plus 46.7 g/d SO, plus 46.7 g/d FO. The results demonstrated that the ruminal C18: 1n-9t proportion was highest in the cattle fed SOFO followed by LOFO, while the ruminal proportion of C18:2n-6 was highest in the cattle fed the SOFO diet. The ruminal C18:3n-3 proportion was highest in the cattle fed the LOFO diet. No significant difference was found for ruminal pH, volatile fatty acids, and ammonia nitrogen (NH3-N), except for NH3-N at 4 h after feeding that was highest in the cattle fed the control diet. Based on the results from the current study, the FO addition to plant oils rich in C18:2n-6 and C18:3n-3 inhibited bio-hydrogenation of C18:2n-6 and C18:3n-3 to C18:0 and yielded trans-11 - C18:1 in the rumen. The increase in trans-11 - C18:1 would increase the conjugated linoleic acid in the meat.
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