PHYTOCHEMICALS AND ANTIOXIDANT PROPERTIES OF WAN KHAN MAK (Aglaonema simplex BL.) FRUIT EXTRACT
Keywords:
Aglaonema simplex Bl, phytochemicals composition, DPPH assay, FRAP assay, DCFH-DA, RAW264.7 cellsAbstract
Aglaonema simplex Bl. (also called wan khan mak (WKM) in Thai), a plant in the family Araceae, has long been claimed in Thai traditional medicine to possess several ethno-pharmacologic properties. Nevertheless, research into its claimed biological activities has never been explored. The present study aimed to investigate the proximate composition, the phytochemicals, and the chemical and intracellular antioxidant activities of 95% ethanol crude extract of the dried fruits of WKM. The results showed that the yield of WKM crude extract was 12.65% of the dry weight, and the contents of total phenolics and flavonoids in the extract contributed to the antioxidant capacities as demonstrated by DPPH and FRAP assays. The WKM extract could also effectively attenuate intracellular reactive oxygen species (ROS) generation in tBuOOH-induced oxidative stress in macrophage RAW264.7 cells, as monitored by using a DCFH-DA fluorescent probe. Overall, the present data suggest that WKM could be a potential source of natural antioxidants and merits further investigation for its possible roles in prevention and/or alleviation of ROS-mediated diseases.
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