INFRARED MICROSPECTROSCOPY TO INVESTIGATE STRUCTURAL CHANGES OF SUBCHONDRAL BONE AND ARTICULAR CARTILAGE IN OSTEOARTHRITIC KNEE
Keywords:
Infrared microspectroscopy, osteoarthritis, articular cartilageAbstract
Osteoarthritis (OA) is characterized by degeneration of the articular cartilage and thickening of the subchondral bone. The aim of this study is to investigate the changing of the biochemical components of the cartilage and bone in OA of the knee by using histochemical staining and synchrotron infrared micro spectroscopy (SR-IR). Three samples were collected from the medial and lateral condyle of the femur. The control group is 21 years old and 47 and 82 years old are the ages of the experimental groups. Histochemical staining was done with hematoxylin and eosin stain, alcian blue. Regarding the results in the articular cartilage, the peak intensities of amide I significantly decreased (0.2073±0.0019 and 0.2070±0.00270) in the 47 years old and82 years old OA knees, respectively, when compared to the normal 21 years old (0.2154±0.0009). The subchondral bone of both (0.2133±0.0008 and 0.2122±0.0007, respectively) were significantly higher than the normal (0.2084±0.0009). Amide II significantly decreased in the82 years old (0.0936±0.0054) compared to the 47 years old (0.1057±0.0013), but was still ata higher level than the normal (0.0854±0.0016). The peak intensity of amide II in the calcified cartilages was not different in all the knees. The peak intensity of proteoglycans was.
References
Aigner, T., Fundel, K., Saas, J., Gebhard, P.M., Haag, J.,Weiss, T., Zien, A., Obermayr, F., Zimmer, R., andBartnik, E. (2006). Large-scale gene expressionprofiling reveals major pathogenetic pathways ofcartilage degeneration in osteoarthritis. ArthritisRheum., 54(11):3533-3544.
Bobinac, D., Spanjol, J., and Zoricic, S. (2003). Changesin articular cartilage and subchondral bonehistomorphometry in osteoarthritic knee joints inhumans. Bone, 32(3):284-290.
Boskey, A. and Camacho, N.P. (2007). FT-IR imaging ofnative and tissue-engineered bone and cartilage.Biomaterials, 28(15):2465-2478.
Chappard, C., Peyrin, F., Bonnassie, A., Lemineur, G.,Brunet-Imbault, B., Lespessailles, E., andBenhamou, C.L. (2006). Subchondral bone microarchitecturalalterations in osteoarthritis: asynchrotron micro-computed tomography study.Osteoarthr. Cartilage,14(3):215-223.
Dragica, B., Josip, S., Sanja, Z., and Ivana, M. (2003).Changes in articular cartilage and subchondral bonehistomorphometry in osteoarthritic knee joints inhumans. Bone, 32:284-290.
Eckstein, F., Milz, S., Anetzberg, H., and Putz, R. (1998).Thickness of the subchondral mineralized tissuezone (SMZ) in normal male and female andpathological human patellae. J. Anat., 192:81-90.
Eckstein, F., Muller-Gerbl, M., Steinlechner, M., Kierse,R., and Putz, R. (1995). Subchondral bone densityof the human elbow assessed by computedtomography osteoabsorptiometry: a reflection of theloading history of the joint surfaces. J. Orthop. Res.,13(2):268-278.
Eckstein, F., Muller-Gerbl, M., and Putz, R. (1992).Distribution of subchondral bone density andcartilage thickness in the human patella. J. Anat.,180:425-433.
Figueiredo, M.M., Gamelas, J.A.F., and Martins, A.G.(2012). Characterization of bone and bone-basedgraft materials using FTIR spectroscopy. In:Infrared Spectroscopy - Life and BiomedicalSciences. Theophile, T. (ed). In Tech, London, UK,p. 315-338.
Goldring, M.B., and Goldring, S.R. (2010). Articularcartilage and subchondral bone in the pathogenesisof osteoarthritis. Ann. NY Acad. Sci., 1192:230-237.
Guilak, F., Ratcliffe, A., Lane, N., Rosenwasser, M.P., andMow, V.C. (1994). Mechanical and biochemicalchanges in the superficial zone of articular cartilagein canine experimental osteoarthritis. J. Orthop.Res., 12:474-484.
Hada, S., Kaneko, H., Sadatsuki, R., Liu, L., Futami, I.,Kinoshita, M., Yusup, A., Saita, Y., Takazawa, Y.,Ikeda, H., Kaneko, K., and Ishijima, M. (2014). Thedegeneration and destruction of femoral articularcartilage shows a greater degree of deteriorationthan that of the tibial and patellar articular cartilagein early stage knee osteoarthritis: a cross-sectionalstudy. Osteoarthr. Cartilage, 22(10):1583-1589.
Hattori, S., Sakane, M., Mutsuzaki, H., Tanaka, J., Ochiai,N., and Nakajima, H. (2007). Chondrocyteapoptosis and decrease of glycosaminoglycan incranial cruciate ligament insertion after resection inrabbits. J. Vet. Med. Sci., 69(3):253-258.
Ijiri, K., Zerbini, L.F., Peng, H., Otu, H.H., Tsuchimochi,K., Otero, M., Dragomir, C., Walsh, N., Bierbaum,B.E., Mattingly, D., van Flandern, G., Komiya, S.,Aigner, T., Libermann, T.A., and Goldring, M.B.(2008). Differential expression of GADD45 innormal and osteoarthritic cartilage: potential role inhomeostasis of articular chondrocytes. ArthritisRheum., 58(7):2075-2087.
Kamibayashi, L., Wyss, U.P., Cooke, T.D.V., and Zee, B.(1995). Trabecular microstructure in the medialcondyle of the proximal tibia of patients with kneeosteoarthritis. Bone, 17:27-35.
Kellgren, J.H. and Lawrence, J.S. (2000). Radiologicalassessment of osteo-arthrosis. Ann. Rheum. Dis.,16(4):494-502.
Khanarian, N.T., Boushell, M.K., Spalazzi, J.P., Pleshko,N., Boskey, A.L., Lu, H.H. (2014). FITI-Icompositional mapping of the cartilage to boneinterface as a function of tissue region and age. J.Bone Miner. Res., 29(12):2643-2652.
Knudson, C.B. and Knudson, W. (2001). Cartilageproteoglycans. Semin. Cell Dev. Biol., 12:69-78.
Kobrina, Y., Rieppo, L., Saarakkala, S., Jurvelin, J.S., andIsaksson, H. (2012). Clustering of infrared spectrareveals histological zones in intact articularcartilage. Osteoarthr. Cartilage, 20(5):460-468.
Kobrina, Y. (2014). Infrared Microspectroscopic ClusterAnalysis of Bone and Cartilage. Dissertations inForestry and Natural Sciences, The University ofEastern Finland, Kuopio, Finland, 103p.
Kumar, R., Gronhaug, K.M., Afseth, N.K., Isaksen, V., deLange Davies, C., Drogset, J.O., and Lilledahl,M.B. (2015). Optical investigation of osteoarthritishuman cartilage (ICRS grade) by confocal Ramanspectroscopy: a pilot study. Anal. Bioanal. Chem.,407:8067-8077.
Lajeunesse, D. (2004). The role of bone in the treatment ofosteoarthritis. Osteoarthr. Cartilage, 12(SupplementA):S34-S38.
Matsui, H., Shimizu, M., and Tsuji, H. (1997). Cartilageand subchondral bone interaction in osteoarthrosisof human knee joint: a histological andhistomorphometric study. Microsc. Res. Techniq.,37(4):333-342.
Muller-Gerbl, M., Putz, R., Hodapp, N., Schulte, E., andWimmer, B. (1989). Computed tomographyosteoabsorptiometryfor assessing the densitydistribution of subchondral bone as a measure oflong term mechanical adaptation in individualjoints. Skeletal Radiol., 18(7):507-512.
Nelson, F., Billinghurst, R.C., Pidoux, I., Reiner, A.,Langworthy, M., McDermott, M., Malogne, T.,Sitler, D.F., Kilambi, N.R., Lenczner, E., and Poole,A.R. (2006). Early post-traumatic osteoarthritislikechanges in human articular cartilage followingrupture of the anterior cruciate ligament.Osteoarthr. Cartilage., 14:114-119.
Oinas, J., Rieppo, L., Finnilä, M.A.J., Valkealahti, M.,Lehenkari, P., and Saarakkala, S. (2016). Imagingof osteoarthritic human articular cartilage usingFourier transform infrared microspectroscopycombined with multivariate and univariate analysis.Sci. Rep-UK, 6:30008.
Panula, H.E., Hyttinen, M.M., Arokoski, J.P., Langsjo,T.K., Pelttari, A., Kiviranta, I., and Helminen, H.J.(1998). Articular cartilage superficial zone collagenbirefringence reduced and cartilage thicknessincreased before surface fibrillation in experimentalosteoarthritis. Ann. Rheum. Dis., 57:237-245.
Potter K, Kidder LH, Levin IW, Lewis EN, Spencer RG.(2001). Imaging of collagen and proteoglycan incartilage sections using Fourier transform infrared.Arthritis Rheum.,44(4):846-55.
Price, J.S., Chambers, M.G., Poole, A.R., Fradin, A., andMason, R.M. (2002). Comparison of collagenase-cleaved articular cartilage collagen in mice in thenaturally occurring STR/ort model of osteoarthritisand in collagen-induced arthritis. Osteoarthr.Cartilage, 10:172-179.
Radin, E.L., Paul, I.L., and Tolkoff, M.J. (1970).Subchondral bone changes in patients with earlydegenerative joint disease. Arthritis Rheum.,13:400-405.
Rieppo, L., Rieppo, J., Jurvelin, J.S., and Saarakkala, S.(2012). Fourier transform infrared spectroscopicimaging and multivariate regression for predictionof proteoglycan content of articular cartilage. PlosOne, 7(2):1-8.
Rizkalla, G., Reiner, A., Bogoch, E., and Poole, A.R.(1992). Studies of the articular cartilageproteoglycan aggrecan in health and osteoarthritis.J. Clin. Invest., 90:2268-2277.
Saarakkala, S., Laasanen, M.S., Jurvelin, J.S., Torronen,K., Lammi, M.J., Lappalainen, R., and Toyras, J.(2003). Ultrasound indentation of normal andspontaneously degenerated bovine articularcartilage. Osteoarthr. Cartilage, 11(9):697-705.
Saarakkala, S., Rieppo, L., Rieppo, J., and Jurvelin, J.S.(2010). Fourier transform infrared (FTIR)microspectroscopy of immature, mature anddegenerated articular cartilage. In: Microscopy:Science, Technology, Applications and Education.Méndez-Vilas, A. and Díaz, J. (eds). FormatexResearch Center, Badajoz, Spain, p. 403-414.
Sabatini, M., Rolland, G., Léonce, S., Thomas, M., Lesur,C., Pérez, V., de Nanteuil, G., and Bonnet, J.(2000). Effects of ceramide on apoptosis,proteoglycan degradation, and matrixmetalloproteinase expression in rabbit articularcartilage. Biochem. Bioph. Res. Co., 267:438-444.
Schmitz, N., Laverty, S., Kraus, V.B., and Aigner, T.(2010). Basic methods in histopathology of jointtissues. Osteoarthr. Cartilage, 18:S113-116.
Schneevoigt, J., Fabian, C., Leovsky, C., Seeger, J., andBahramsoltani, M. (2017). In vitro expression of theextracellular matrix components aggrecan, collagentype I and II by articular cartilage-derivedchondrocytes. Anat. Histol. Embryol., 46(1):43-50.
Sindhupakorn, B., Thienpratharn, S., and Kidkhunthod, P.(2017). A structural study of bone changes in kneeosteoarthritis by synchrotron-based X-rayfluorescence and X-ray absorption spectroscopytechniques. J. Mol. Struct., 1,146:254-258.
Stoop, R., Buma, P., van der Kraan, P.M., Hollander, A.P.,Billinghurst, R.C., Meijers, T.H., Poole, A.R., andvan den Berg, W.B. (2001). Type II collagendegradation in articular cartilage fibrillation afteranterior cruciate ligament transection in rats.Osteoarthr. Cartilage, 9:308-315.
Takahashi, Y., Sugano, N., Takao, M., Sakai, T., Nishii,T., and Pezzotti, G. (2014). Raman spectroscopyinvestigation of load-assisted microstructuralalterations in human knee cartilage: preliminarystudy into diagnostic potential for osteoarthritis. J.Mech. Behav. Biomed., 31:77-85.
Temple, M.M., Bae, W.C., Chen, M.Q., Lotz, M., Amiel,D., Coutts, R.D., and Sah, R.L. (2007). Age- andsite-associated biomechanical weakening of humanarticular cartilage of the femoral condyle.Osteoarthr. Cartilage, 15(9):1042-1052.
