SOLID STATE CULTIVATION OF THE SELECTED FUNGUS STRAINS FOR PROTEIN INCREMENT IN FRESH CASSAVA PULP
Keywords:
Cassava pulp, solid state cultivation, high protein content, Aspergillus sp, Trichoderma spAbstract
Solid state cultivation of the 3 selected fungal strains, Aspergillus sp. CASP01 and UN03, and Trichoderma sp. CASP02, was performed to increase the protein content in fresh cassava pulp. The fungi were isolated from cassava pulp, and exhibited over-production of cellulase, amylase, and pectinase. For increasing protein content in fresh cassava pulp, the addition of 1 of these 4 nitrogen sources, namely soybean meal, yeast extract, ammonium sulfate, and urea, at the equivalent concentration of 2% nitrogen, enhanced growth of Aspergillus sp. CASP01 and UN03, and at 1% nitrogen stimulated growth of Trichoderma sp. CASP02. Soybean meal was selected as the most suitable nitrogen source. From solid state cultivation of the selected 3 fungal strains in a packed bed bioreactor using fresh cassava pulp (600 g with 85% moisture content and 2% original crude protein) supplemented with soybean meal, Aspergillus sp. UN03 provided the highest protein increase (13.36% crude protein yield) after 36 h cultivation. The achieved results reveal the promising process for feed production and the management of solid waste in a value-added bio-product.
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