ANTI-STAPHYLOCOCCAL SCREENING OF SELECTED THAI MEDICINAL PLANTS FROM NAKHON RATCHASIMA PROVINCE
Keywords:
Methicillin-resistant S, aureus, S. aureus, anti-staphylococcal activity, plant extractAbstract
To investigate and evaluate the anti-staphylococcal activity of selected plants which used in Thai traditional medicine, twenty-six ethanolic extracts of 24 selected Thai medicinal plant species were prepared. An antibacterial activity of the extracts was evaluated forStaphylococcus aureus and Methicillin-resistant S. aureus (MRSA) using paper disc agardiffusion method and broth microdilution method. Of the 26 extracts tested, four plantextracts including, whole plants of Limnophila aromatica (Lam.) Merr, leaves of Piper betleLinn., leaves of Rhodomyrtus tomentosa (Aiton) Hassk., and leaves of Xanthostemon youngiiC.T.White & W.D.Francis produced inhibition zones on S. aureus and MRSA. Theinhibition zones were ranged from 8 mm to 13 mm. Then, minimal inhibitory concentration(MIC) and minimal bactericidal concentration (MBC) values of all extracts were determined. An ethanolic extract of R. tomentosa demonstrated as the most effective plantwith MIC and MBC values of 0.31/0.62 and 0.62/0.62 mg/ml against S. aureus and MRSA,respectively. In addition, MIC values of P. betle, X. youngii and L. aromatica against S.aureus and MRSA ranged from 1.25-2.5 mg/ml whereas the extracts had the MBCs value ranging from 1.25-5.0 mg/ml. The results from this study showed the four plant extracts acted as an alternative source of anti-staphylococcal activity against S. aureus and itsresistant strain.
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