SCREENING OF BIOACTIVE COMPOUNDS FROM BACTERIAL ENDOPHYTES ISOLATED FROM MICROPROPAGATED PLANTS
Abstract
Bacterial endophytes are microorganisms inhabit in plant tissues. Additionally, they are able to produce effective bioactive compounds leading to apply in agricultural, industrial and medical fields. This study was carried on isolation of bacterial endophytes from riceand pineapple cultivated in micropropagation, identification of bacterial strains and evaluation for bioactive compounds production including antimicrobial activities, Indoleacetic-acid (IAA) production and phosphate solubilization ability. Fourteen isolates of bacterial endophytes were obtained for the present study. Antimicrobial activities againstsix strains of human pathogenic bacteria and phytopathogenic fungi were investigated.Three isolates of bacterial endophytes from pineapple were able to inhibit the growth of Bacillus cereus and Salmonella typhimurium. Then five bacterial endophyte isolates exhibited the growth inhibitory activity on Aspergillus sp., Collectrichum sp., Campylocarponfasciculare and Rhizopus nigricans. Furthermore, evaluation of plant growth promoting activities including IAA production and the ability of phosphate solubilizing were examined. Results showed that all of sixteen bacterial isolates exhibited IAA production activity ranged from 0.38 μg/ml to 237.5 μg/ml nutrient broth supplemented with 200 μg/mlof tryptophan. Additionally, eight isolates of bacterial endophytes possessed the phosphate solubilization ability and the highest solubilization index was 2.8.
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