LIGHT MICROSCOPIC OBSERVATIONS OF THE MESENTERO- PROCTODEAL REGIONS IN ADULT CATOPSILLA POMONA (FABRICIUS, 1758) (LEPIDOPTERA: PIERIDAE)

Pisit  Poolprasert; Ezra  Mongkolchaichana; Sinlapachai  Senarat; Jes  Kettratad; Watiporn  Yenchum; Waristha  Angsirijinda

Authors

Pisit Poolprasert Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok, 65000, Thailand.
Ezra Mongkolchaichana Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok, 65000, Thailand.
Sinlapachai Senarat Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Jes Kettratad Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Watiporn Yenchum Bio-Analysis Laboratory, Department of Chemical Metrology and Biometry, National Institute of Metrology (Thailand), Pathum Thani, 10120, Thailand.
Waristha Angsirijinda Department of Veterinary Medicine, Mahanakorn University of Technology, Bangkok, 10530, Thailand.

Keywords:

Catopsilia pomona, mesentero-proctodeal regions, histology, Thailand

Abstract

The aim of this study was to examine the basic histological structure of the mesentero-proctodeal regions (midgut and hindgut) of Catopsilia pomona (Fabricius, 1758) which were living in their natural habitat in the northern part of Thailand. All specimens were processed by the standard histological technique. Each section was cut at 6 micrometers and stained with Harris's haematoxylin and eosin. For the longitudinal sections, the results revealed that the structural wall in both the midgut and hindgut could be divided obviously into 4 layers: the mucosa, submucosa, muscularis, and serosa, respectively. The midgut was mainly shown to be 2 types of epithelial cells, based on histological localization: (i) a simple columnar epithelium and (ii) a simple cuboidal epithelium. The hindgut was classified distinctly into 2 subparts: the ilium and the rectum. The ilium was a simple squamous epithelium, whereas the rectum was a simple squamous epithelium with 5-6 rectal papillae. The results of this study showed the details of the histology of the mesentero-proctodeal regions and are considered the first such report in Thailand.

References

Arab, A. and Caetano, F.H. (2002). Segmental specializations in the Malpighian tubules of the fire ant Solenopsis saevissima Forel 1904 (Myrmicinae): an electron microscopical study. Arthropod Struct. Dev., 30:281-292.

Areekul, S. (1957). The comparative internal larval anatomy of several genera of Scarabaeidae (Coleoptera). Ann. Entomol. Soc. Am., 50:562- 577.

Bancroft, J.D. and Gamble, M. (2002). Theory and Practice of Histological Techniques. Churchill Livingstone, London, 744p.

Beals, G.R. and Berberet, R.C. (1976). Anatomy and histology of selected systems in larval and adult Elasmopalus lingosellus (Lepidoptera: Pyralidae). Ann. Entomol. Soc. Am., 69:1,105-1,119.

Bordas, L. (1920). Etude anatomique et histologique de l’appareil digestif des Lipidopteres. Ann. Sci. Nat. Zool., 3:175-250.

Burgess, E. (1880). Contributions to the anatomy of the milkweed butterfly, Danias archippus. Anniv. Mem. Boston Soc. Nat. Hist., 1-16.

Caetano, F.H. and Overal, W.L. (1984). Estudos do trato digestivo de vespas (Hymenoptera, Vespidae) e estruturas excretoras associadas. I. Morfologia. Rev. Bras. Entomol., 28:403-408.

Chauthani, A.R. and Callahan, P. S. (1967). Developmental morphology of the alimentary canal of Heliothes zea (Lepidoptera: Noctuidae). Ann. Entomol. Soc. Am., 60:1,136-1,140.

Cruz-Landim, C. (1985). Ultra-estrutura e função do tubo digestivo dos insetos. Proceedings of the Eighth Annual Symposium of the Academy of Sciences of the State of São Paulo (ACIESP); October 9-14, 1983; São Paulo, Brazil, ACIESP 44, p. 28-49.

Ek-Amnuay, P. (2006). Butterflies of Thailand. Baan LaeSuan - Amarin Printing and Publishing Co, Bangkok, Thailand, 849p.

Fabricius, J.C. (1775). Systema Entomologyiae, Sistens Insectorum Classes, Ordines, Genera, Species, Adiectis Sysnonymis, Locis, Descriptionibus, Observationibus, Officina Libraria Kortii, Flensburg and Leipzig, Germany, 832p.

Food and Agriculture Organization of the United Nations (FAO), (2007). Overview of forest pests – Thailand. Forest Health & Biosecurity Working Papers FBS/32E, Forestry Department, FAO, Rome, Italy, 43 p.

Gillott, C. (1995). Entomology. Plenum Press, New York, NY, USA, 798p.

Gongalves, I.S. (1981). Anatomia do tubo digestive de Pseudaletia sequax Franclemont 1951 (Lepidoptera, Noctuidae). Rev. Bras. Entomol., 25: 113-121

Goyle, S. (1990). Anatomy of the Common Lemon Butterfly, Papilio Demoleus Demoleus (L). Today & Tomorrow’s Printers and Publishers, New Delhi, India, p. 151.

Irvine, B., Audsley, N., Lechleitner, R., Meredith, J., Thomson, B., and Phillips, J. (1988). Transport properties of locust ileum in vitro: Effects of cyclic AMP. J. Exp. Biol., 137:361-385.

Judy, K.J. and Gilbert, I.L. (1970). Morphology of the alimentary canal during the metamorphosis of Hyalophora cecropia (Lepidoptera: Saturniidae) Ann. Entomol. Soc. Am., 62:1,438-1,446.

Kabir, A. and Ameen, M. (1986). Anatomy and histology of the alimentary canal of adult Diacrisia obliqua Walker (Lepidoptera: Arctiidae). Bangaladesh J. Zool., 14:49-57.

Lacombe, D. (1971). Anatomy and histology of Embolyntha batesi Maclachian, 1877 (Embiidina). Mem. I. Oswaldo Cruz, 69:331-390.

Le Grice, D.S. (1968). Some observations on the morphology and histology of the alimentary canal of the fruit-sucking moth Serrodes inara Cram. (Lepidoptera: Noctuidae). S. Afr. J. Agric. Sci., 11:789-796.

Mathur, L.M.L. (1972). Developmental changes in the alimentary canal of Prodenia litura Fabr. (Lepidoptera). J. Nat. Hist., 6:39-46.

Mortimer, T.J. (1965). The alimentary canals of some adult Lepidoptera and Trichoptera. T. Roy. Ent. Soc. Lond., 117:67-93.

Pyle, R.W. (1940). The anatomy and histology of the digestive system of a supposedly non-feeding adult moth, Callosomia promethean Dur. (Lepidoptera: Saturniidae). Entomol. News, 51:181-185.

Raju, A.J.S., Lakshmi, P.V., Ramana, K.V., and Chandra, P.H. (2012). Entomophily, ornithophily and anemochory in the self- incompatible Boswellia ovalifoliolata Bal. & Henry (Burseraceae), an endemic and endangered medicinally important tree species. Journal of Threatened Taxa, 4(7):2,673-2,684.

Roy, S., Gayen, A.K., Mitra, B., and Duttagupta, A. (2014). Diversity, foraging activities of the insect visitors of Mustard (Brassica juncea Linnaeus) and their role in pollination in West Bengal. The Journal of Zoology Studies, 1(2):7-12.

Schumaker, T.T.S., Cristofoletti P.T., and Terra, W.R. (1993). Properties and compartmentalization of digestive carbohydrases and proteases in Scaptotrigona bipunctata (Apidae, Meliponinae) larvae. Apidologie, 24:3-17.

Serrão J.E., Marques-Silva, S., and Martins, G.F. (2004). The rectum of Oxaea flavescens (Andrenidae) has a specialized structure among bees. Micron, 35:245-253.

Serrão, J.E. and Cruz-Landim, C. (1996). A comparative study of digestive cells in different midgut regions of stingless bees (Hymenoptera Apidae: Meliponinae). J. Adv. Zool., 17:1-6.

Standlea, P.P. and Yonke, T.R. (1968). Clarification of the description of the digestive system of Heliothis zea. Ann. Entomol. Soc. Am., 61:147-148.