EFFECTS OF MET HYDROXY ANALOG (MHA®) SUPPLEMENTATION OF DAIRY COW’S DIETS ON MILK YIELD AND MILK COMPOSITION
Keywords:
Met hydroxy analog, milk production, milk composition, milk fatty acidsAbstract
Twenty one Holstein Friesian crossbred (>87.5% Holstein Friesian) lactating dairy cows, averaging 103 + 53 days in milk, 12.5 + 3.0 kg of milk, 58 + 19 mo old and 412 + 56 kg bodyweight (BW), were blocked by parity first and then stratified random balanced for milking days,milk yield, age and body weight into three groups of 7 cows. The first group (control) received approximately 17.4 kgDM of total mixed ration (TMR) which comprised approximately 7, 6,and 30 kg of commercial concentrate, corn silage and fresh cut grass, respectively. The second group was fed with the basal diet as the control group and supplemented with 11 g/d of Met hydroxy analog (MHA®). The third group was fed the same way as the control group with 22 g/dof Met hydroxy analog (MHA®). Performance parameters showed that DM, CP, and NEL intakes, final body weight and live weight change were similar in all treatments. Milk yield and milk composition were unaffected, however, UFA was increased while SFA was reduced byMHA® supplementation.
References
AOAC (Association of Official AnalyticalChemists). (1995). Official Methods of Analysis. 16th ed. Association of OfficialAnalytical Chemists, Arlington, VA.
Baumrucker, C.R. (1985). Amino acid transport systems in bovine mammary tissue. J.Dairy Sci., 68:2436.
Bhargava, P.K., Otterby, D.E., Murphy, J.M.,and Donker, J.D. (1977). Methioninehydroxyl analog in diets for lactating cows. J. Dairy Sci., 60:1594-1604.
Chalupa, W. (1975). Rumen bypass and protection of proteins and amino acids.J. Dairy Sci., 58:1198-1218.
Clark, J.I.L. (1975). Lactational responses to post ruminal administration of proteinsand amino acids. J. Dairy Sci., 58:1178.
Folch, J., Lees, M., and Stanley, G.H.S. (1957).A simple method for the isolation and purification of total lipides from animaltissues. J. Biol. Chem., 226:497-509
Graulet B., Richard, C., and Robert, J.C.(2005). Methionine availability inplasma of dairy cows supplemented with methionine hydroxyl analog isopropyl ester. J. dairy sci., 88:3640-3649.
Hansen, W.P., Otterby, D.E., Linn, J.G., andDonker, J.D. (1991). Influence of forage type, ratio of forage to concentrate, and methionine hydroxy analog on performance of dairy cows. J. DairySci., 74:1361–1369.
Kaufmann, V.W. and Lupping, W. (1979). The effect of protected protein and HMM-Caon milk yield of dairy cows. J. Anim.Physiol. Anim. Nutr., 41:202-217.
Kaufmann, W. and Lupping, W. (1982).Protected proteins and protected amino acids for ruminants. In: Protein Contribution of Feedstuffs for Ruminants:Application to Feed Formulation. Miller,E.L., Pike, I.H. and Van Es, A.J.H.(eds.), Studies in the Agricultural and Food Sciences, Butterworth Scientific, Washington, D.C., p. 36-75.
Lundquist, R., Bhargava, P.K., Linn, J.G., andOtterby, D.E. (1982). Methionine hydroxy analog for lactating dairy cattle Proc.43rd Minnesota Nutr. Conference, University of Minnesota Extension Service, Minneapolis, MN., p. 31-35.
Metcalfe, D.D., Astwood, J.D., Townsend, R.,Sampson, H.A., Taylor, S.T., and Fuchs,R.L. (1996). Assessment of allergenic potential of foods derived from genetically engineered crop plants. Crit. Rev. FoodSci. Nut., 36:S165-S186.
National Research Council. (2001). Nutrient Requirements of Dairy Cattle. 7th rev.ed. Nat. Acad. Press. Washington D.C.,408p.
Noftsger, S., St-Pierre, N.R., and Sylvester,J.T. (2005). Determination of rumen degradability and ruminal effects of three sources of methionine in lactating cows. J. Dairy Sci., 88:223–237.
Oldham, J.D. (1984). Protein and energy relationship in dairy cows. J. DairySci., 67:1090-1114.
Ostrowska, E., Dunshea, F.R., Muralitharan,M., and Cross, R.F. (2000). Comparison of Siliverion high performance liquid chromatographic quantification of free and methylated conjugated linoleic acid.Lipids., 35:1147-1153.
Pisulewski, P.M., Rulquin, H., Peyraud J.L,and Verite R. (1996). Lactational and systemic responses to postruminalinfusions of increasing amounts of methionine in dairy cows. J. Dairy Sci.,79:1781-1791.
Polan, C.E., Chandler, P.T., and Miller, C.N.(1970). Methionine hydroxy analog:varyios levels for lactating cows. J.Dairy Sci., 53:607.
Robinson, P.H., Fredeen, A.H., Chalupa, W.,Julien, W.E., Sato, H., Fujieda, T., and Suzuki, H. (1995). Ruminally protected lysine and methionine for lactating dairycows fed a diet designed to meet requirements for microbial and postruminalprotein. J. Dairy Sci., 78:582-594.
Rulquin, H. and Delaby, L. (1977). Effects ofthe energy balance of dairy cows on lactational responses to rumen-protected methionine. J. Dairy Sci., 80:2513-2522.
Rulquin, H., Pisulewski, P.M., Ve´rite´, R.,and Guinard, J. (1993). Milk production and composition as a function ofpostruminal lysine and methionine supply: a nutrient-response approach.Livest. Prod. Sci., 37:69-90.
SAS/STAT User’s Guide, (1996). 4th ed. SASInst. Inc., Cary, NC, USA, 474p.
Schingoethe, D.J., Casper, D.P., Yang, C.,lUg, D.J., Sommeffelth, J.L., and Mu¢ller, C.R. (1988). Lactational response tosoybean meal, heated soybean meal, andextruded soybeans with ruminallyprotected methionine. J. Dairy Sci.,71:173.
Schwab, C.G. (1995). Protected proteins and amino acids for ruminants. In:Biotechnology in Animal Feeds andAnimal Feeding. Wallace R.J. andChesson, A., (eds.). VCH Verlagsgesellschaft,Weinheim (Federal Republic ofGermany) and VCH Publishers Inc.,NY., p. 115-141.
Schwab, C.G., Bozak, C.K., Whitehouse,N.L., and Mesbah, M.M.A. (1992).Amino acid limitation and flow to duodenum at four stages of lactation.1. Sequence of lysine and methionine limitation. J. Dairy Sci., 75:3486–3502.
Spires, H.R., Clark, J.H., Derrig, R.G., andDavis, C.L. (1975). Milk production and nitrogen utilization in response to post ruminal infusion of sodium caseinate in lactating cows. J. Nutr., 105:1111-1121.
St-Pierre and Sylvester, J.T. (2005). Effects of2-hydroxy-4-(methylthio) butanoic acid(HMB) and its isopropyl ester on milk production and composition by holsteincows. J. Dairy Sci., 88:2487–2497.
Steel, R.G.D. and Torries, J.H. (1980).Principles and Procedures of Statistics:A Biometeric Approach. 2nd Ed.McGrowHill: NY, p. 401-437.
Stern, M.D., Rode, L.M., Prange, R.W.,Stauffacher, G.H., and Satter, L.D.(1983). Ruminal protein degradation of corn gluten meal in lactating dairy cattle fitted with duodenal T-type cannulae. J.Anim. Sci., 56:194.
Stokes, M.R., Clark, J.H., and Steinmetz,L.M. (1981). Performance of lactating dairy cows fed methionine or methionine analog at two concen trations of dietary crude protein. J. Dairy Sci., 64:1686-1694.
Suksombat, W. and Chullanandana, K. (2008).Effects of soybean oil or rumen protected conjugated linoleic acid supplementation on accumulation of conjugated linoleic acid in dairy cows’ milk. Asian-Aust. J.Anim. Sci., 21(9):1271-1277.
Třináctý, J., Křížová, L., Hadrová, S., Hanuš,O., Janštová, B., Vorlová, L., and Dračková, M., (2006). Effect of rumen-protected supplement with three amino acids onmilk yield, composition and fatty acids profile in dairy cows. J. Anim. Sci.,15:3-15
Vanhatalo, A., Huhtanen, P., Toivonen, V., andVarvikko, T. (1999). Response of dairy cows fed grass silage diets to abomasa infusions of histidine alone or in combinations with methionine and lysine. J. Dairy Sci., 82:2674-2685.
Van Soest, P. J., Robertson, J.B., and Lewis,B. A. (1991). Methods for dietary fiber, neutral detergent fiber and no starch polysaccharides in relation to animal production. J. Dairy Sci., 74:3583-3597








