ANTIOXIDANT PROPERTIES OF PUERARIN AND GENISTEIN FROM WHITE KWAO KRUA INDUCED BY ELICITORS AND THEIR ANTIHYPERGLYCEMIC EFFECT ON RATS
Keywords:
White Kwao Krua, elicitors, antioxidant activity, isoflavonoids, antihyperglycemicAbstract
White Kwao Krua [Pueraria candollei Grah. ex Benth. var. mirifica (A.Shaw and Suvatabandhu)Niyomdham] is a Thai native medicinal plant with a long record of traditional medicinal consumptionamong menopausal women for the purposes of rejuvenation and estrogen replacement. It is attractinggreat interests due to the antioxidant and estrogen-like properties of some of its natural products.The antioxidant activities of puerarin and genistin in White Kwao Krua (WKK) that were treatedwith elicitors, and their antihyperglycemic effect on rats (Rattus norvegicus) were investigated. Eightcombinations of chitosan, CuCl2 and salicylic acid (SA) were applied over two months to forty eightplants of WKK grown in a growth chamber. The results showed that all combinations of additives hadstatistically significant effects on antioxidant activities and the amount of genistein and puerarinproduced. The combination of chitosan (1000 mg/L) + CuCl2 (200 mg/L) + salicylic acid (100 mg/L)gave the highest antioxidant activities [IC50 value = 2482 μg/ml for DPPH, FRAP value = 45.5 μmolFe2+/g dry weight (dw)], while the combination of chitosan (1000 mg/L) + CuCl2 (200 mg/L) gave thehighest amount of genistein (423 μg/g dw) and puerarin (22.6 μg/g dw). WKK crude extract couldreduce blood sugar level in streptozotocin (STZ)-diabetic rats. The results showed that the elicitorsused can increase the amount of puerarin and genistein and their antioxidant activities in WKK.Furthermore, WKK crude extract pronounced an antihyperglycemic effect in STZ-diabetic rats also.
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