DEVELOPMENT OF COMBINED ULTIMATE HYBRID RESPIROMETER-TITRATE METER TO ESTIMATE KINETIC PARAMETERS OF ACTIVATED SLUDGE
Keywords:
Activated sludge, kinetic, oxygen uptake rate, respirometry, titrimetryAbstract
The aim of this paper is to present a new development and an application of the ultimate hybridrespirometer-titrate meter for wastewater characterization and kinetic parameters estimation ofmicroorganisms in activated sludge. Currently, respirometry and titrimetry are considered highperformance tools with accuracy and frequency measurement. A new implementation of ultimatehybrid respirometer combined with titrate meter was developed. The new set-up of instrument wasdeveloped based on the previous knowledge and needed only sensor electrodes. The user interfaceprogramming to system control and monitor was developed based on LabVIEW 8.2 (student edition)software packages equipped with NI DAQ USB-6210 (A/D 16 bit). Finally, a laboratory implementationwas tested with readily biodegradable substrate, such as sodium acetate and ammonium chloride, toestimate the essential kinetic parameters of heterotrophic as yield, maximum specific growth rate, decayrate, saturation constant for substrate and initial ammonium concentration in activated sludge samples.The parameter estimation results from using respirometric measurements data were close to the defaultvalues in Activated Sludge Model No. 1 (ASM1) and other reported values. The ammonium concentrationcalculate by using base information were much correlated with the amount of ammonium applied to theactivated sludge sample. This demonstrated that the new instrument set-up was successfully applied toestimate kinetic parameters of activated sludge and measurement of ammonium concentration inactivated sludge samples.
References
Artiga, P., Gonzalez, F., Mosquera-Corral, A.,Campos, J.L., Garrido, J.M., Ficara, E.,and Mendez, R. (2005). Multiple analysisreprogrammable titration analyser for thekinetic characterization of nitrifying andautotrophic denitrifying biomass. J.Biochem. Eng., 26:176-183.
Cech, J.S., Chudoba, J., and Grau, P. (1984).Determination of kinetic constants ofactivated sludge microorganisms. WaterSci. Technol., 17:259-272.
Dosta, J. (2007). Operation and model descriptionof advanced biological nitrogen removaltreatments of highly ammonium loadedwastewater, [Ph.D. thesis]. Departmentd’Enginyeria Quimica, UniversitatAutonoma de Bacelona. Spain, 288p.
Dricks, K., Pind, P.f., Mosbaek, H., and Henze,M. (1999). Yield determination byrespirometry-The possible influence ofstorage under aerobic conditions inactivated sludge. Water SA., 25(1):69-74.
Gernaey, K., Borgaert, H., Massone, A.,Vanrolleghem, P.A., and Verstraete, W.(1997). On-line nitrification monitoring inactivated sludge with a titrimetric sensor.Environ. Sci. Technol., 31(8):2,350-2,355.
Gernaey, K., Petersen, B., Nopens, I., Comeau,Y., and Vanrolleghem, P.A. (2002a). Modellingaerobic carbon source degradationprocesses using titrimetric and combinedrespirometric-titrimetric data: Experimentaldata and model structure. Biotech.Bioeng., 79(7):741-753.
Gernaey, K., Petersen, B., Nopens, I., Dochain,D., and Vanrolleghem, P.A. (2002b).Modelling aerobic carbon source degradationprocesses using titrimetric andcombined respirometric-titrimetric data:Structural and practical identifiability.Biotech. Bioeng., 79(7):754-767.
Gernaey, K., Petersen, B., Ottoy, J.P., andVanrolleghem, P.A. (2001). Activatedsludge monitoring with combined respirometric-titrimetric measurement. Wat. Res.,35(5):1,280-1,294.
Gernaey, K., Vanrolleghem, P.A., and Verstraete,W. (1998). On-line estimation ofNITROSOMONAS kinetic parameters inactivated sludge samples using titrationin-sensor experiments. Wat. Res.,32(1):71-80.
Guisasola, A. (2005). Modelling biologicalorganic matter and nutrient removalprocesses from wastewater using respirometricand titrimetric techniques, [Ph.D.thesis]. Department d’EnginyeriaQuimica, Universitat Autonoma deBacelona. Spain, 337p.
Guisasola, A., Vargas, M., Marcelino, M.,Lafuente, J., Casas, C., and Baeza, J.A.(2007). On-line monitoring of theenhanced biological phosphorus removalprocess using respirometry and titrimetry.J. Biochem. Eng., 35:371-379.
Henze, M., Grady Jr., C.P.L., Gujer, W., Marais,G.V.R., and Matsuo, T. (2000). Activatedsludge models: ASM1, ASM2, ASM2dand ASM3. IWA Scientific and TechnicalReports No. 9. London, IWA, 130p.
Jeppsson, U. (1996). Modelling aspects ofwastewater treatment process, [Ph.D.thesis]. Department of IndustrialElectrical Engineering and Automation,Lund Institute of Technology, Sweden,428p.
Jubany, I. (2007). Operation, modelling andautomatic control of complete and partialnitrification of highly concentratedammonium wastewater, [Ph.D. thesis].Department d’Enginyeria Quimica,Universitat Autonoma de Bacelona, Spain,270p.
Kappeler, J. and Gujer, W. (1992). Estimationof kinetic parameters of heterotrophicbiomass under aerobic conditions andcharacterization of wastewater for activatedsludge modelling. Water Sci. Technol.,25(6):125-139.
Lu, P., Zhang, D., Zhang, X., and Cao, H. (2006).An implementation of the hybrid respirometricmeasurement principle withmore reliable oxygen uptake rate (OUR)measurement. Water Practice & Technology,1(4). Available from: www.iwaonline.com.
Manser, R., Gujer, W., and Siegrist, H. (2006).Decay processes of nitrifying bacteria inbiological wastewater treatment system.Wat. Res., 40:2,416-2,426.
Massone, A., Gernaey, K., Borgaert, H.,Vanderhasselt, A., Rozzi, A., andVerstraete, W. (1996). Biosensors fornitrogen control in wastewaters. WaterSci. Technol., 34(1-2):213-220.
Muller, A.W., Wentzel, M.C., and Ekama, G.A.(2004). Experimental determination of theheterotroph anoxic yield in anoxicaerobicactivated sludge systems treatingmunicipal wastewater. Water SA., 30(5):7-12.
Onnis, A., Carucci, A., and Cappai, G. (2006).Titration biosensore for the estimation ofthe biological nitrate demand of municipaland industrial wastes. J. Ind MicrobiolBiotechnol., 33:243-246.
Orhon, D. and Artan, N. (1994). Modelling ofactivated sludge systems. Pennsylvania,Technomic, 589p.
Penya-Roja, J.M., Seco, A., Ferrer, J., andSerralta, J. (2002). Calibration andvalidation of activated sludge model no.2d for Spanish municipal wastewater.Environ. Technol., 23:849–862.
Petersen, B. (2000). Calibration, identifiabilityand optimal experimental design ofactivated sludge models, [Ph.D. thesis].Faculty of Bioscience Engineering, GhentUniversity, Belgium, 362p.
Ramadori, R., Rozzi, A., and Tandoi, V. (1980).An automated system for monitoringthe kinetics of biological oxidation ofammonia. Wat. Res., 14:1,555-1,557.
Rozzi, A., Remigi, E., and Burkley, C. (2001).Methanogenic activity measurements bythe MAIA biosensor: Instructions guide.Water Sci. Technol., 44(4):287-294.
Sin, G. and Vanrolleghem, P.A. (2007). Extensionsto modeling aerobic carbon degradationusing combined respirometric-titrimetricmeasurements in view of activated sludgemodel calibration. Wat. Res., 41:3,345-3,358.
Spanjers, H. and Vanrolleghem, P.A. (1995).Respirometry as a tool for rapidcharacterisation of wastewater andactivated sludge. Water Sci. Technol.,31(2):105-114.
Spanjers, H., Vanrolleghem, P.A., Olsson, G.,and Dold, P.L. (1998). Respirometry incontrol of the activated sludge process:Principles. IWA Scientific and TechnicalReport No. 7. London, IWA, 48p.
Vanrolleghem, P.A. and Spanjers, H. (1998). Ahybrid respirometric method for morereliable assessment of activated sludgemodel parameters. Water Sci. Technol.,37(12):237-246








