THE USE OF SSR MARKERS TO IDENTIFY HETEROTIC PATTERN OF F1 HYBRIDS IN TWO TROPICAL MAIZE POPULATIONS
Keywords:
Maize, simple sequence repeats (SSR), population, heterosis, genetic distance (GD), marker assisted selection (MAS)Abstract
The objectives of this study were to (i) select lines from two tropical maize populations using geneticdistance (GD) with 50 simple sequence repeats (SSR) markers for F1 hybrid production and (ii) studythe relationship between GD and yield and GD and mid-parent heterosis (MPH), using SSR markers.The results showed that the GD of the S1 selected lines from the two maize populations ranged from0.14 to 0.94, with the average of 0.44, which manifested the high genetic diversity among the S1 selectedlines. The grain yield of the F1 hybrids obtained from the crosses between the S1 selected lines of bothpopulations was evaluated. The mean grain yield of all F1 hybrids was 8,865 kgha-1. The F1 hybridsfrom the crosses between G2001 × Y1008 and G2068 × Y1002 gave the highest grain yields of 10,799kgha-1 and 10,721 kgha-1, respectively. The GD showed a positive correlation with the grain yield of theF1 hybrids and the MPH with the values of 0.21 and 0.07, respectively. However our research showed alow correlation between the GD and F1 hybrid grain yield and the MPH, because there was a differencein the linkage disequilibrium among the markers used and the quantitative trait loci (QTLs) betweenthe heterotic groups. This may be related to DNA markers being able to effectively predict hybridperformance with DNA heterozygosity. However the difference and number of markers studied mayhave also reflected the degree of correlation. The number of hybrids evaluated and the first generationof selfing in the lines studied may have also affected the value of the correlation.
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