CULTURAL CHARACTERISTICS OF SPHACELOMA AMPELINUM, CAUSAL PATHOGEN OF GRAPE ANTHRACNOSE ON DIFFERENT MEDIA
Keywords:
Elsinoe ampelina, Sphaceloma ampelinum, culture medium, grapeAbstract
Sphaceloma ampelinum, the causal pathogen of grape anthracnose, is the anamorph stage of Elsinoeampelina. To evaluate its variability, isolates of S. ampelinum were collected from diseased plants fromthe northeastern, northern, eastern, and western regions of Thailand. The pathogen was isolated by atissue transplanting method on water agar (WA) and the growing mycelium was subsequentlytransferred onto cereal agar (CA) and susceptible grape leaves to induce sporulation. Single conidialisolates were obtained and 19 representatives from all regions were cultured on potato dextrose agar(PDA), CA, corn cereal agar (CCA) and Job’s tear corn cereal agar (JCCA) for cultural characterization.It was found that the different culture media affected growth, colony color, appearance, and formationof aerial mycelium. The best culture media for surface mycelium growth was CA, while PDA led to thehighest aerial mycelium formation. In addition, CA induced the highest morphological variability amongisolates. Variation of isolates appeared to be more pronounced among different regions than that withinthe same regions, except isolates from the eastern region. By using cultural characteristics alone, someisolates within the same region cannot be fully differentiated. These results suggest that diversity existsamong isolates of S. ampelinum in Thailand, particularly those from different regions.
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