EFFECT OF CULTURE CONDITIONS ON BACTERIAL CELLULOSE (BC) PRODUCTION FROM ACETOBACTER XYLINUM TISTR976 AND PHYSICAL PROPERTIES OF BC PARCHMENT PAPER
Keywords:
Acetobacter xylinum, optimization, bacterial cellulose, parchment paperAbstract
In this study, Acetobacter xylinum strain TISTR976 was cultured in coconut juice base medium underthree culture conditions: static tray, shaking flask, and a 5 liter stirring bioreactor, with optimal pH4.5 at 30°C. From these three culturing conditions, BC yields of 4.73, 5.32, and 7.94 g/liter wereobtained on day 6 of cultivation. By using a specific process, the BC from the three conditions wasmade into paper called BC paper. The BC papers produced from three culture conditions were testedfor physical properties, and studied for micro-structures by a scanning electron microscope (SEM).The physical properties of the BC papers, such as wet strength retention were figured out as 124.2,25.7, and 12.2%, respectively, however, the oil resistant value of the bioreactor BC paper was so poorin comparison with that of the static tray and shaking flask. From this evidence, it was shown thatonly BC paper from the static tray and shaking flask exhibited the characteristics of specialtyparchment paper. Furthermore, the SEM micrographs of BC paper from the static tray and shakingflask showed the crystalline fibril layers with an orderly fibril layer arrangement, whereas thatfrom the bioreactor was disorderly. A hypothesis on the correlation between the parchment paperproperty and the orderly fibril layer arrangement of BC paper has been notified.
References
Borzani, W. and de Souza, S.J. (1995). Mechanismof the film thickness increasingduring the bacterial production of celluloseon non-agitated liquid media.Biotechnol. Lett., 17:1,271-1,272.
Brown, Jr. R.M. (1992). Emerging technologiesand future prospects for industrializationof microbially derived cellulose.In: Harnessing Biotechnology for the 21stCentury. Ladisch, M.R. and Bose, A. (ed).Proceedings of the 9th InternationalBiotechnology Symposium and Exposition.Crystal City, Virginia. AmericanChemical Society, Washington, D.C.,p. 76-79.
Cannon, R.E. and Anderson, S.M. (1991).Biogenesis of bacterial cellulose. Crit.Rev. Microbiol., 17:435-447.
Casey, J.P. (1960). Pulp and Paper Chemistry andChemical Technology. Vol. 2, 2nd ed.Interscience, NY, 1,249p.
Iguchi, M., Yamanaka, S., and Budhiono, A.(2000). Bacterial cellulose - a masterpieceof nature’s arts. J. Mater. Sci., 35:261-270.
Jonas, R. and Farah, L.F. (1998). Production andapplication of microbial cellulose. Polym.Degrad. Stab., 59:101-106.
Masaoka, S., Ohe, T., and Sakota, N. (1993).Production of cellulose from glucose byAcetobacter xylinum. J. Ferment. Bioeng.,75:18-22.
Okiyama, A., Motoki, M., and Yamanaka, S.(1993). Bacterial cellulose IV. Applicationto processed foods. Food Hydrocolloids,6:503-511.
Smook, G.A. (1990). Handbook of Pulp andPaper Terminology. 1st ed. Angus WildeBellingham, Washington, D.C., 447p.
Suwannapinunt, N., Sookson, R., andLaosangtham, D., inventors; Departmentof Science Service and the NationalResearch Council of Thailand, assignee.July 24, 2001. Bacterial cellulose parchmentpaper with oil resistant property andprocess. Thai Petty Patent no. 306.
Technical Association of Pulp and PaperIndustry (TAPPI). (1998). TAPPI Press,Atlanta, Georgia. TAPPI Test method:T410 om-98, T411-om-97, T404 om-92, T456om-96, T414 om-98, T460 om-96, T462om-93.
Watanabe, K. and Yamanaka, S. (1995). Effectsof oxygen tension in the gaseous phaseon production and physical properties ofbacterial cellulose formed under staticculture conditions. Biosci. Biotechnol.Biochem., 59:65-68.
Watanabe, K., Tabuchi, M., Morinaga, Y., andYoshinaga, F. (1998). Structural featuresand properties of bacterial celluloseproduced in agitated culture. Cellulose,5:187-200.
Yamanaka, S., Watanabe, K., Kitamura, N.,Iguchi, M., Mitsuhashi, S., Nishi, Y., andUryu, M. (1989). The structure andmechanical properties of sheets preparedfrom bacterial cellulose. J. Mater. Sci.,24:3,141-3,145.
