POTENTIAL APPLICATION OF WASTEWATER FROM RICE NOODLE MANUFACTURE IN α -AMYLASE PRODUCTION
Keywords:
Amylase, wastewater, rice noodle, Aspergillus spAbstract
The feasibility of using wastewater from rice noodle manufacture as a substrate for the production of α-amylase was evaluated. The maximum α-amylase production by Aspergillus sp. NU4 was achieved after 24 h. Supplementation of appropriate nitrogen sources and inorganic salts to the wastewater medium significantly enhanced enzyme production. The enzyme yield reached a maximum of 36.5 U/ml with the wastewater adding with 10 g/l defatted soybean, 10 g/l KH2PO4, 5 g/l MgSO4.7H2O, and 0.1 g/l ZnCl2. It accounted for 8-fold increase. The α-amylase of Aspergillus sp. NU4 was subjected to catabolite repression. It was partially purified using 60% ammonium sulfate. The optimum pH and temperature of the partially purified enzyme were determined to be 5 and 50°C, respectively. After 2-h incubation, the enzyme degraded more than 90% of raw starch granules of potato starch, rice starch, and corn starch.
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