PHYSICAL PROPERTIES AND BIOACTIVITIES OF CURCUMIN-LOADED IN-SITU IMPLANT
Physical Properties and Bioactivities of Curcumin-Loaded In-Situ Implant
DOI:
https://doi.org/10.55766/sujst10615Keywords:
Curcumin, In Situ Implant, Borneol, Ibuprofen, Bioactivity PropertiesAbstract
Curcumin, the important active component in turmeric extracts, has gained significant attention demonstrating notable effectiveness across a wide range of human pathological conditions. This study explores the development and characterization of borneol and ibuprofen-based in situ implant (ISI) systems loaded with curcumin, designed for advanced therapeutic applications. Curcumin ISI solution enabled self-transformation into matrix at 40% w/w concentration. Ibuprofen and borneol enhanced curcumin transformation at lower concentration. These ISI systems exhibited low viscosity and swift transformation aiding for their administration by injection. Macroscopic and cross-sectional imaging provided insights into their phase inversion process. Curcumin-loaded borneol-based ISI demonstrated high antioxidant activity. Both curcumin-loaded borneol-based and ibuprofen-based ISIs showed efficient antimicrobial activities against related periodontitis pathogens. These findings underscore the potential of curcumin-loaded borneol and ibuprofen-based ISI systems as promising candidates for localized drug delivery, particularly in treating periodontitis.
References
Amato, M., Santonocito, S., Polizzi, A., Tartaglia, G.M., Ronsivalle, V., Viglianisi, G., Grippaudo, C., and Isola, G. (2023). Local delivery and controlled release drugs systems: A new approach for the clinical treatment of periodontitis therapy. Pharmaceutics, 15(4):1312. https://doi.org/10.3390/pharmaceutics15041312
Batool, F., Agossa, K., Lizambard, M., Petit, C., Bugueno, I. M., Delcourt-Debruyne, E., Benkirane-Jessel, N., Tenenbaum, H., Siepmann, J., Siepmann, F., and Huck, O. (2019). In-situ forming implants loaded with chlorhexidine and ibuprofen for periodontal treatment: Proof of concept study in vivo. International Journal of Pharmaceutics, 569:118564. https://doi.org/10.1016/j.ijpharm.2019.118564
Baliyan, S., Mukherjee, R., Priyadarshini, A., Vibhuti, A., Gupta, A., Pandey, R.P., and Chang, C.M. (2022). Determination of antioxidants by DPPH radical scavenging activity and quantitative phytochemical analysis of Ficus religiosa. Molecules, 27(4):1326. https://doi.org/10.3390/molecules27041326
Brayton, C.F. (1986). Dimethyl sulfoxide (DMSO): A review. Cornell Veterinarian, 76(1):61-90.
Chantadee, T., Santimaleeworagun, W., Phorom, Y., Chuenbarn, T., and Phaechamud, T. (2020). Saturated fatty acid-based in situ forming matrices for localized antimicrobial delivery. Pharmaceutics, 12(9):808. https://doi.org/10.3390/pharmaceutics12090808
Chantadee, T., Sirirak, J., Hoshino, T., and Phaechamud, T. (2021). Augmentative molecular aspect for phase inversion of vancomycin hydrochloride-loaded fatty acid in situ forming matrices. Materials and Design, 199:109429. https://doi.org/10.1016/j.matdes.2020.109429
Chaiya, P., Senarat, S., Phaechamud, T., and Narakornwit, W. (2022). In vitro anti-inflammatory activity using thermally inhibiting protein denaturation of egg albumin and antimicrobial activities of some organic solvents. Materials Today: Proceedings, 65:2,290-2,295. https://doi.org/10.1016/j.matpr.2022.04.916
Chen, Z.X., Xu, Q.Q., Shan, C.S., Shi, Y.H., Wang, Y., Chang, R.C.C., and Zheng, G.Q. (2019). Borneol for regulating the permeability of the blood-brain barrier in experimental ischemic stroke: Preclinical evidence and possible mechanism. Oxidative Medicine and Cellular Longevity, 2019:2936737. https://doi.org/10.1155/2019/2936737
Chuenbarn, T., Sirirak, J., Tuntarawongsa, S., Okonogi, S., and Phaechamud, T. (2022). Design and comparative evaluation of vancomycin HCl-loaded rosin-based in situ forming gel and microparticles. Gels, 8(4):231. https://doi.org/10.3390/gels8040231
Cozmin, M., Lungu, I.I., Gutu, C., Stefanache, A., Duceac, L.D., Șoltuzu, B.D., Damir, D., Calin, G., Bogdan Goroftei, E.R., Grierosu, C., and Boev, M. (2024). Turmeric: From spice to cure. A review of the anti-cancer, radioprotective and anti-inflammatory effects of turmeric sourced compounds. Frontiers in Nutrition, 11:1399888. https://doi.org/10.3389/fnut.2024.1399888
Eldeeb, A.E., Salah, S., Mabrouk, M., Amer, M.S., and Elkasabgy, N.A. (2022). Dual-drug delivery via zein in situ forming implants augmented with titanium-doped bioactive glass for bone regeneration: Preparation, in vitro characterization, and in vivo evaluation. Pharmaceutics, 14(2):274. https://doi.org/10.3390/pharmaceutics14020274
Fogueri, L.R. and Singh, S. (2009). Smart polymers for controlled delivery of proteins and peptides: A review of patents. Recent Patents on Drug Delivery and Formulation, 3:40-48. https://doi.org/10.2174/187221109787158300
Gad, H.A., El-Nabarawi, M.A., and Abd El-Hady, S.S. (2008). Formulation and evaluation of PLA and PLGA in situ implants containing secnidazole and/or doxycycline for treatment of periodontitis. AAPS PharmSciTech, 9:878-884. https://doi.org/10.1208/s12249-008-9126-9129
Grimling, B., Meler, J., Szcześniak, M., Pluta, J., and Górniak, A. (2015). The study of physicochemical properties of solid dispersions of ibuprofen in the presence of chitosan. Progress on Chemistry and Application of Chitin and Its Derivatives, 20:64-72.
Guo, Y., Yan, S., Xu, L., Zhu, G., Yu, X., and Tong, X. (2014). Use of Angong Niuhuang in treating central nervous system diseases and related research. Evidence-Based Complementary and Alternative Medicine, 2014:1-9. https://doi.org/10.1155/2014/346918
Irvine, J., Afrose, A., and Islam, N. (2018). Formulation and delivery strategies of ibuprofen: Challenges and opportunities. Drug Development and Industrial Pharmacy, 44(2):173-183. https://doi.org/10.1080/03639045.2017.1391838
Jin, J., Ruohua, Z., Hongmin, L., Jie, Z., Yongzheng, P., and Qin, G. (2020). Analgesic and anti-inflammatory effects and mechanism of action of borneol on photodynamic therapy of acne. Environmental Toxicology and Pharmacology, 75:103329. https://doi.org/10.1016/j.etap.2019.103329
Jouyban, A., Fakhree, M.A.A., and Shayanfar, A. (2010). Review of pharmaceutical applications of N-methyl-2-pyrrolidone. Journal of Pharmacy and Pharmaceutical Sciences, 13(4):524. https://doi.org/10.18433/j3p306
Khaing, E.M., Lertsuphotvanit, N., Thammasut, W., Rojviriya, C., Chansatidkosol, S., Phattarateera, S., Pichayakorn, W., and Phaechamud, T. (2024). Cellulose acetate butyrate-based in situ gel comprising doxycycline hyclate and metronidazole. Polymers, 16:3477. https://doi.org/10.3390/polym16243477
Lei, C., Jianyu, S., Lin, L., Bing, L., and Wang, L. (2011). A new source of natural D-borneol and its characteristic. Journal of Medicinal Plants Research, 5(15):3440-3447.
Lertsuphotvanit, N., Santimaleeworagun, W., Narakornwit, W., Chuenbarn, T., Mahadlek, J., Chantadee, T., and Phaechamud, T. (2022). Borneol-based antisolvent-induced in situ forming matrix for crevicular pocket delivery of vancomycin hydrochloride. International Journal of Pharmaceutics, 617:121603. https://doi.org/10.1016/j.ijpharm.2022.121603
Li, J.M., Hu, S.X., and Li, H.Q. (2013). The commodity types and historical origins of traditional Chinese medicine borneol. Modern Chinese Medicine, 2013(6).
Peng, Y., Ao, M., Dong, B., Jiang, Y., Yu, L., Chen, Z., Hu, C., and Xu, R. (2021). Anti-inflammatory effects of curcumin in the inflammatory diseases: Status, limitations and countermeasures. Drug Design, Development and Therapy, 15:4,503-4,525. https://doi.org/10.2147/DDDT.S327378
Phaechamud, T., Mahadlek, J., Charoenteeraboon, J., and Choopun, S. (2012). Characterization and antimicrobial activity of N-methyl-2-pyrrolidone-loaded ethylene oxide-propylene oxide block copolymer thermosensitive gel. Indian Journal of Pharmaceutical Sciences, 74(6):498-504. https://doi.org/10.4103/0250-474X.110574
Phaechamud, T., Mahadlek, J., and Tuntarawongsa, S. (2018a). Peppermint oil/doxycycline hyclate-loaded Eudragit RS in situ forming gel for periodontitis treatment. Journal of Pharmaceutical Investigation, 48, 451-464. https://doi.org/10.1007/s40005-017-0340-x
Phaechamud, T., Lertsuphotvanit, N., and Praphanwittaya, P. (2018b). Viscoelastic and thermal properties of doxycycline hyclate-loaded bleached shellac in situ-forming gel and -microparticle. Journal of Drug Delivery Science and Technology, 44:448-456. https://doi.org/10.1016/j.jddst.2018.01.021
Phaechamud, T., Thurein, S.M., and Chantadee, T. (2018c). Role of clove oil in solvent exchange-induced doxycycline hyclate-loaded Eudragit RS in situ forming gel. Asian Journal of Pharmaceutical Sciences, 13(2):131-142. https://doi.org/10.1016/j.ajps.2017.09.004
Phaechamud, T., Senarat, S., Puyathorn, N., and Praphanwittaya, P. (2019). Solvent exchange and drug release characteristics of doxycycline hyclate-loaded bleached shellac in situ-forming gel and -microparticle. International Journal of Biological Macromolecules, 135:1,261-1,272. https://doi.org/10.1016/j.ijbiomac.2018.11.098
Puyathorn, N., Lertsuphotvanit, N., Chantadee, T., Pichayakorn, W., and Phaechamud, T. (2023a). Lincomycin HCl-loaded borneol-based in situ gel for periodontitis treatment. Gels, 9(6):495. https://doi.org/10.3390/gels9060495
Puyathorn, N., Tamdee, P., Sirirak, J., Okonogi, S., Phaechamud, T., and Chantadee, T. (2023b). Computational insight of phase transformation and drug release behaviour of doxycycline-loaded ibuprofen-based in-situ forming gel. Pharmaceutics, 15(9):2315. https://doi.org/10.3390/pharmaceutics15092315
Puyathorn, N., Senarat, S., Lertsuphotvanit, N., and Phaechamud, T. (2023c). Physicochemical and bioactivity characteristics of doxycycline hyclate-loaded solvent removal-induced ibuprofen-based in situ forming gel. Gels, 9(2):128. https://doi.org/10.3390/gels9020128
Rein, S. M. T., Lwin, W. W., Tuntarawongsa, S., and Phaechamud, T. (2020). Meloxicam-loaded solvent exchange-induced in situ forming beta-cyclodextrin gel and microparticle for periodontal pocket delivery. Materials Science and Engineering: C, 117:111275. https://doi.org/10.1016/j.msec.2020.111275
Rungseevijitprapa, W. and Bodmeier, R. (2009). Injectability of biodegradable in situ forming microparticle systems (ISM). European Journal of Pharmaceutical Sciences, 36:524-531. https://doi.org/10.1016/j.ejps.2008.12.003 https://doi.org/10.1016/j.ejps.2008.12.003
Sabira, S., Munir, N., Mahmood, Z., Riaz, M., Akram, M., Rebezov, M., Kuderinova, N., Moldabayeva, Z., Shariati, M.A., Rauf, A., and Rengasamy, K.R.R. (2021). Molecular targets for the management of cancer using Curcuma longa Linn. phytoconstituents: A review. Biomedicine and Pharmacotherapy, 135:111078. https://doi.org/10.1016/j.biopha.2020.111078
Saw, C.L., Olivo, M., Wohland, T., Fu, C.Y., Kho, K.W., Soo, K.C., and Sia Heng, P.W. (2007). Effects of N-methyl pyrrolidone on the uptake of hypericin in human bladder carcinoma and co-staining with DAPI investigated by confocal microscopy. Technology in Cancer Research and Treatment, 6:383-394. https://doi.org/10.1177/153303460700600503
Schwach-Abdellaoui, K., Vivien-Castioni, N., and Gurny, R. (2000). Local delivery of antimicrobial agents for the treatment of periodontal disease. European Journal of Pharmaceutics and Biopharmaceutics, 50:83-99. https://doi.org/10.1016/S0939-6411(00)00086-2
Senarat, S., Tuntarawongsa, S., Lertsuphotvanit, N., Rojviriya, C., Phaechamud, T., and Chantadee, T. (2023a). Levofloxacin HCl-loaded Eudragit L-based solvent exchange-induced in situ forming gel using monopropylene glycol as a solvent for periodontitis treatment. Gels, 9:583. https://doi.org/10.3390/gels9060583
Senarat, S., Rojviriya, C., Sarunyakasitrin, K., Charoentreeraboon, J., Pichayakorn, W., and Phaechamud, T. (2023b). Moxifloxacin HCl-incorporated aqueous-induced nitrocellulose-based in situ gel for periodontal pocket delivery. Gels, 9:572. https://doi.org/10.3390/gels9070572
Urošević, M., Nikolić, L., Gajić, I., Nikolić, V., Dinić, A., and Miljković, V. (2022). Curcumin: Biological activities and modern pharmaceutical forms. Antibiotics, 11(2):135. https://doi.org/10.3390/antibiotics11020135
