DNA Extraction from Oyster Mushrooms Fruiting Bodies without Mycelial Activation Process
Keywords:
Oyster Mushrooms, Molecular Marker, ITS, HAT-RAPD, DNAAbstract
Background and Objectives: Oyster mushrooms (Pleurotus spp.) are economically valuable mushrooms with high market demand. They are popularly consumed worldwide due to their highly palatable taste and numerous medicinal properties. Despite the many benefits of oyster mushrooms, information regarding the genetic diversity of these mushrooms is limited; investigation into their diversity is of utmost importance. While molecular marker techniques, along with the study of phenotypes, are popular approaches, the use of these techniques necessitates the use of high-quality DNA. However, the process of stimulating mycelium growth is complex and time-consuming. A direct DNA extraction method from oyster mushroom fruiting bodies was therefore developed in this present study.
Methodology: For the method of DNA extraction from oyster mushrooms, we adjusted the composition of the extraction reagents to suit the samples by modifying the original extraction methods. The quality and quantity of the DNA were assessed using a Nanodrop Lite spectrophotometer, while the DNA quality was evaluated in reactions using ITS and HAT-RAPD techniques.
Main Results: The results demonstrated clear and high-quality DNA band patterns, enabling the identification and classification of oyster mushroom species.
Conclusions: The direct DNA extraction method from oyster mushrooms, using a modified approach, demonstrated that the extracted DNA was of high quality and purity, suitable for PCR reactions. Furthermore, when the DNA quality was analyzed for classification using ITS and HAT-RAPD techniques, clear and appropriately sized bands were obtained.
Practical Application: This modified extraction method allows for rapid direct DNA extraction from oyster mushroom fruiting bodies. It is also suitable for rare, deteriorated and limited quantity samples. Consequently, this method can be used to investigate the genetic diversity of oyster mushroom strains.
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