Phytochemical Compositions and Biological Activities of Essential Oil and Crude Extract of Sesuvium portulacastrum

Rittipun Rungruang; Oratai Kokilakanishtha; Orrapun Selamassakul; Nattapon Kaisangsri; Orapin Kerdchoechuen; Nutta Laohakunjit

Authors

Rittipun Rungruang Department of Cosmetic Science, Faculty of Science and Technology, Suan Dusit University, Bangkok, Thailand
Oratai Kokilakanishtha Research and Development Institute, Suan Dusit University, Bangkok, Thailand; Research Innovation Management Unit, Suan Dusit University, Bangkok, Thailand
Orrapun Selamassakul Agricultural and Functional Foods Processing Laboratory, Pilot Plant Development and Training Institute, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand
Nattapon Kaisangsri Agricultural and Functional Foods Processing Laboratory, Pilot Plant Development and Training Institute, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand
Orapin Kerdchoechuen Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand
Nutta Laohakunjit Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand

Keywords:

Sesuvium portulacastrum, SPME-GC-MS, Antioxidant Activity, Anti-microbial, Cytotoxicity

Abstract

This research aimed to study phytochemical compositions of essential oil and crude extracts of Sesuvium portulacastrum. Results showed that total phenolics contents of the crude extract and essential oil were 31.57±1.83 and 8.03±1.44 mg GAE/g extract, respectively. Three major compounds, as analyzed by HPLC, were found in crude extract, namely, epicatechin, vanillic acid and 2-hydroxycinnamic acids (o-Coumaric acid). The essential oil was analyzed by SPME-GC-MS and 2-methoxy-4-vinylphenol (2M4VP), β-damascenone and terpene were found. In terms of bioactivities, the crude extract exhibited higher antioxidant, metal chelating and anti-collagenase activities than the essential oil. When antimicrobial activity was tested, results showed that crude extract could inhibit Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli. On the other hand, the essential oil could inhibit S. aureus, E. coli and Candida albicans. These results revealed that the crude extract of S. portulacastrum has higher efficacy than the essential oil; crude extract did not show toxicity against CCD-986SK cells, of which the survival percentage of the cells was 87.28±6.21%.

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