Efficacy of Vegetable Crude Extracts to Inhibit Bacteria, Exiguobacterium Indicum
Keywords:antibacterial, Chinese kale, crude extract, Exiguobacterium indicum, pumpkin, yard-long bean
Crude vegetable extracts are very interesting to study and then applied to inhibit bacteria. In this study, we obtained crude extracts from vegetable wastes, including yard-long bean leaf, pumpkin peel, and Chinese kale leaf, and studied their efficacies of those crude extracts to inhibit the bacteria, and investigated their phytochemicals using Gas chromatography-mass spectrometry. The efficacy of the crude vegetable extracts in inhibiting bacteria was evaluated based on the size of the inhibition zone. The results showed that the pathogenic bacteria isolated from the infected Nile tilapia fish was Exiguobacterium indicum presenting yellow colony. This species showed the highest inhibition activity at 15.33 mm from Chinese kale leaf crude extract. After testing antibacterial activity, crude extract from Chinese kale leaf had the highest antibacterial efficiency; however, there was no significant difference (P>0.05) with yard-long bean leaf extract. Phytochemical studies of all three vegetable wastes showed that crude extracts from Chinese kale leaves and yard-long bean leaves contained high quantities of 3,7,11,15-Tetramethyl-2-hexadecen-1-ol Phytol, 9,12,15-Octadecatrienoic acid, (Z,Z,Z)-, Butyl 9,12,15-octadecatrienoate, and Ethyl 9,12 ,15-octadecatrienoate in high quantity to antimicrobials, which are antimicrobials. In contrast, upon studying the phytochemical compounds, it was found that the crude extracts from yard-long bean leaves contained the highest number of various compounds. This study indicated that the crude extracts from Chinese kale leaf and yard-long bean leaf can be applied in preventing pathogenic bacteria in aquaculture, especially Nile tilapia fish
Ahmed, O. M., Fahim, H. I., Ahmed, H. Y., Al-Muzafar, H. M., Ahmed, R. R., Amin, K. A., ... & Abdelazeem, W. H. (2019). The Preventive Effects and the Mechanisms of Action of Navel Orange Peel Hydroethanolic Extract, Naringin, and Naringenin in N-Acetyl-p-aminophenol-Induced Liver Injury in Wistar Rats. Oxidative Medicine and Cellular Longevity, 2019, Article 2745352. https://doi.org/10.1155/2019/2745352.
Al-Marzoqi, A. H., Sahi Al-Khafaji, N. M., & Hussein, H. J. (2016). In vitro Antibacterial Activity Assessment of the crude Phenolic, Alkaloid and Terpenoid compounds extracts of Lepidium sativum L. on Human Pathogenic Bacteria. International Journal of ChemTech Research, 9(4), 529-532.
Altschul, S. F., Madden, T. L., Schaeffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research, 25(17), 3389–3402. https://doi.org/10.1093/nar/25.17.3389
Brosius, J., Dull, T. J., Sleeter, D. D., & Noller, H. F. (1981). Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. Journal of Molecular Biology, 148(2), 107–127. https://doi.org/10.1016/0022-2836(81)90508-8
Chakraborty, S. B., & Hancz, C. (2011). Application of phytochemicals as immunostimulant, antipathogenic and antistress agents in Finfish Culture. Review in Aquaculture, 3(3), 103–119. https://doi.org/10.1111/j.1753-5131.2011.01048.x
Chaturvedi, P. & Shivaji, S. (2006). Exiguobacterium indicum sp. nov., a psychrophilic bacterium from the Hamta glacier of the Himalayan Mountain ranges of India. International Journal of Systematic and Evutionary Microbilogy, 56(12), 2765-2770. https://doi.org/10.1099/ijs.0.64508-0
Chitmanat, C., Tongdonmuan, K., & Nunsong, W. (2005). The use of crude extracts from traditional medicinal plants to eliminate Trichodina sp. in Nile tilapia (Oreochromis niloticus) fingerlings. Songklanakarin Journal of Science and Technology, 27(1), 359–364.
Devakumar, B., Halappa, P., & Shivakumara, C. (2017). Dy3+/Eu3+ co-doped CsGd(MoO4)2 phosphor with tunable photoluminescence properties for near-UV WLEDs applications. Dyes and Pigments, 137, 244-255. https://doi.org/10.1016/j.dyepig.2016.10.016.
Enerijiofi, K. E., Akapo, F. H., & Erhabor, J.O. (2021). GC–MS analysis and antibacterial activities of Moringa oleifera leaf extracts on selected clinical bacterial isolates. Bulletin of the National Research Centre, 45, 1-10. https://doi.org/10.1186/s42269-021-00640-9
Ghosh, G., Panda, P., Rath, M., Pal, A., Sharma, T., & Das, D. (2015). GC-MS analysis of bioactive compounds in the methanol extract of Clerodendrum viscosum leaves. Pharmacognosy Research, 7(1), 110–113. https://doi.org/10.4103/0974-8490.147223
Harikrishnan, R., Balasundaram, C. & Heo, M.S. (2011). Impact of plant products on innate and adaptive immune system of cultured finfish and shellfish. Aquaculture, 317(1-4), 1-15. https://doi.org/10.1016/j.aquaculture.2011.03.039
Hazarika, S., Goswami, N. Dutta, N. N., & Hazarika, A. (2002). Ethyl oleate synthesis by porcine pancreatic lipase in organic solvent. Journal of Chemical Engineering, 85(1), 61–68. https://doi.org/10.1016/S1385-8947(01)00144-9
Kadhim, K. L., Kadhim, H. B., & Lami, F. H. (2017). Prevalence, Incidence, Trend, and Complications of Thalassemia in Iraq. Hemoglobin, 41(3), 164-168. https://doi.org/10.1080/03630269. 2017.1354877
Kamolrat, N., & Chopjit, P. (2018). Effect of Mao juice (Antidesma sp.) to Preventing Streptococcus spp. in Nile tilapia (Oreochromis niloticus). Agricultural Science Journal, 49(3), 182–186.
Katsura, K., Kawasaki, H., Potacharoen, W., Saono, S., Seki, T., Yamada, Y., Uchimura, T.., & Komagata, K. (2001). Asaia siamensis sp. nov., an acetic acid bacterium in the α-Proteobacteria. International Journal of Systematic and Evolutionary Microbiology, 51(2), 559–563. https://doi.org/10.1099/00207713-51-2-559
Kawasaki, H., Hoshino, Y., Hirata, A., & Yamasato, K. (1993). Is intracytoplasmic membrane structure a generic criterion? It does not coincide with phylogenetic interrelationships among photosynthetic purple non-sulfur bacteria. Archives of Microbiology, 160, 358–362. https://doi.org/10.1007/BF00252221
Lisa, K. A., Alam, S. M., Jubayer, A. A., & Mone, H. F. (2021). Antagonistic activity of Exiguobacterium indicum LIS01 isolated from sediment. Journal of Advance Biotechnology and Experimental Therapeutics, 4(2), 114–119. https://doi.org/10.5455/jabet.2021.d112
Mongkolvai, P., Insuwan, W., Nanuam, J., Bunjun, K., Phosai, W., Thanomsit, C., … & Phimchan, P. (2021). Phytochemical and protein extract of Gloden Shower Tree (Cassia fustula), Naresuan University Journal: Science and Technology, 29(1), 21–35.
Muthulakshmi, A., Jothibai Margret, R., & Mohan, V. R., (2012). GC-MS Analysis of Bioactive Components of Feronia elephantum Correa (Rutaceae). Journal of Applied Pharmaceutical Science, 02(02), 69–74.
Myers, E. W. & Miller, W. (1988). Optimal alignments in linear space. Computer Applications in the Biosciences, 4(1), 11–17. https://doi.org/10.1093/bioinformatics/4.1.11
Naiumsawang, W. (2019). Isolation and Screening of Bacterial Antagonistic for Controlling Aspergillus flavus. Wichcha Journal, 38(1), 28-41.
National Center of Biotechnology Information. (2022, September 25). Basic Local Alignment Search Tool. https://blast.ncbi.nlm.nih.gov/Blast.cgi
Nithikulworawong, N. (2012). Efficacy of Bauhinia sirinhorniae on resistance to against Streptococcus agalactia in Nile tilapia (Oreochromis niloticus), KKU Research Journal, 17(5), 715-724.
Potiwong, C., Suwan, C., Naimoon, P., Yawchai, P., Jitmanowan, S., & Chitmanat, C. (2020). Effects of medical plants on Fish immunity and its growth performances. Burapha Science Journal, 25(2), 595–619.
Raman, B. V., Samuel, L. A., Saradhi, M. P., Rao, B. N., Krishna, N. V., Sudhakar, M., & Radhakrishnan, T. M. (2012). Antibacterial, antioxidant activity and GC-MS analysis of Eupatorium odoratum. Asian Journal of Pharmaceutical and Clinical Research, 5(2), 99-106.
Rattanachaikunsopon, P. & Phumkhachorn P. (2009). Prophylactic effect of Andrographis paniculata extracts against Streptococcus agalactiae infection in Nile tilapia (Oreochromis niloticus). Journal of Bioscience and Bioengineering, 107(5), 579- 582.
Reverter, M., Bontemps, N., Lecchini, D., Banaigs, B. & Sasal, P. (2014). Use of plant extracts in fish aquaculture as an alternative to chemotherapy: current status and future perspectives. Aquaculture, 433, 50-61. https://doi.org/10.1016/j.aquaculture.2014.05.048
Shaheed, K. A., AlGaraawi, N. I., Alsultany, A. K., Abbas, Z. H., Khshayyish, I. K., & Al Khazali, M. T. (2019). Analysis of bioactive phytochemical compound of (Cyperus iria L.) By using gas chromatography-mass spectrometry. IOP Conference Series: Earth and Environmental Science, 388(1), 12–64. https://doi.org/10.1088/1755-1315/388/1/012064
Sirikhansaeng, P., Tanee, T., Sudmoon, R., & Chaveerach, A. (2017). Major phytochemical as γ-sitosterol disclosing and toxicity testing in Lagerstroemia species. Evidence-Based Complementary and Alternative Medicine, 2017, Article 7209851. https://doi.org/10.1155/2017/7209851.
Siswadi, S., & Saragih, G. S. (2021, May 25). Phytochemical analysis of bioactive compounds in ethanolic extract of Sterculia quadrifida R.Br [Conference presentation]. AIP Conference Proceedings, Malang, Indonesia. https://doi.org/10.1063/5.0053057
Tyagi, T., & Agarwal, M., (2017). Phytochemical screening and GC-MS analysis of bioactive constituents in the ethanol extract of Pistia stratiotes L. and Eichhornia crassipes (Mart.) solms. Journal of Pharmacognosy and Phytochemistry, 6(1), 195–206.
White, A. R., Soles, A. S., Gavelis. G., Gosselin, E., Slater, F. G., Lim, S. D., Leander, B., & Suttle A. C. (2019). The complete genome of physiology analysis of the eurythermal firmicute Exiguobacterium chiriqhucha strain RW2 isolated from a freshwater microbialite, widely adaptable to broad thermal, pH and salinity ranges. Frontiers in Microbiology, 9, 1–20. https://doi.org/10.3389/fmicb.2018.03189
Yamada, Y., Katsura, K., Kawasaki, H., Widyastuti, Y., Saono, S., Seki, T., ... & Komagata, K. (2000). Asaia bogorensis gen. nov., sp. nov., an unusual acetic acid bacterium in the alpha -Proteobacteria. International Journal of Systematic and Evolutionary Microbiology, 50(2), 823–829. https://doi.org/10.1099/00207713-50-2-823
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