Effects of y-irradiation on free radicals, active components and toxicity of  Turmeric rhizomes

Piyanuch Thongphasuk; Jarunee Thongphasuk

Authors

Piyanuch Thongphasuk Department of Pharmacognosy, Faculty of Pharmacy, Rangsit University, Patumthani 12000, Thailand
Jarunee Thongphasuk Research and Development Division, Thailand Institute of Nuclear Technology (Public Organization), Nakornnayok 26120, Thailand

Keywords:

gamma irradiation, turmeric, volatile oil, free radicals, antioxidant activity, total phenolic content

Abstract

Gamma irradiation is a method utilized to improve safety of medicinal herbs by inactivation of microorganisms. Since irradiation may also affect active compounds and toxicity of the irradiated herbs, the objective of this research is to study the effects of $gif.latex?\gamma$ -irradiation (10 and 25 kGy) on turmeric (Curcuma longa L.). GC-MS, HPLC and Electron paramagnetic resonance spectroscopy (EPR) were used to determine volatile oil content, curcuminoid content and free radicals, respectively. Total phenolic content and free radical scavenging activity were investigated by spectroscopic techniques. Toxicity was determined by Toxi-Chromo Test. The results showed that g-irradiation at the doses of 10 and 25 kGy significantly (P < 0.05) increased free radicals. However, volatile oil content, curcuminoid content, total phenolic content, free radical scavenging activity and toxicity were not significantly (P > 0.05) affected by the irradiation doses.

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