Effect of different mashing on non-alcoholic beer quality from Thai rice malt


  • Yupakanit Puangwerakul Faculty of Food Technology, College of Agricultural Innovation, Food and Biotechnology Rangsit University, Patumthani 12000, Thailand
  • Maneerat Lekpan Faculty of Biotechnology, College of Agricultural Innovation, Food and Biotechnology Rangsit University, Patumthani 12000, Thailand
  • Napaporn Khamhaeng Faculty of Biotechnology, College of Agricultural Innovation, Food and Biotechnology Rangsit University, Patumthani 12000, Thailand


mashing, infusion mash, sugar composition, rice beer, non-alcoholic beer, healthy beverage


The effect of mashing temperature on the quality of wort and beer from Thai rice malt was studied using two non-glutinous rice varieties; Homnin and PathumThani 1.  The mashing program was carried out at two different temperatures, 65° and 75°C, for the sugar rest period.  It was found that the chemical properties of wort from infusion mash at 65° and 75°C were not different in % extract, reducing sugar and free amino nitrogen contents.  However, sugar composition from different worts was significantly different.  Sugar composition of wort from infusion mash at 65°C gave 8.65 g/100g maltose and 0.98 g/100g glucose while at 75°C gave 3.26 g/100g maltose and 6.70 g/100g glucose.  Wort fermentation was prepared in batch sizes of 20 litre (L) using yeast Saccharomyces cerevisiae RSU no.10.  The beer qualities of color value, turbidity, pH, and reducing sugar were not different.  It was also found that the chemical properties of both beer patterns were the same in quantities of Free Amino Nitrogen (FAN), % extract, % alcohol and bitterness.  The bio-functional compounds content corresponded to specification of a healthy beer product which focused on antioxidant, phenolic compound and GABA and were not significantly different, except for the thiamine content remaining in the final product.  It was found that thiamine content in beer from infusion mash at 75°C wort was higher than beer from infusion mash at 65°C wort.  The sensory evaluation of beers indicated that appearance, aroma, taste, mouth-feel and overall impression of non-alcoholic beer from both beer patterns were judged as drinkable and preferred to the commercial non-alcoholic beer from Germany, namely Clausthaler.  The results obtained from this research clearly indicated that non-alcoholic beer from Thai rice malt influenced perception of the consumer and could be used as alternative healthy beverage with an acceptable quality.


AOAC. (2000). Official Methods of Analysis (17th ed). The Association of Official Analytical Chemists. Arlington, Virginia, USA: AOAC International.

Alvarez, E., Correa, J. M., Navaza, J. M., & Riverol, C. (2000). Injection of steam into the mashing process as alternative method for the temperature control and low-cost of production. Journal of Food Engineering, 43(4), 193-196. DOI: 10.1016/S0260-8774(99)00144-2

Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of free radical method to evaluate antioxidant activity. Lebensmittel-Wissenschaft & Technologie, 28(1), 25-30.

Briggs, D. E., Hough, J. S., Stevens, R., & Young, T. W. (1981). Malting and brewing science volume 1: Malt and sweet wort (2nd ed). Weimar, Texas, USA: Culinary and Hospitality Industry Publications Services (C.H.I.P.S.).

Cadenas, E., & Packer, L. (2002). Antioxidants in health and disease. In E. Cadenas & L. Packer Handbook of antioxidants, second edition revised and expand. New York, NY, USA: Marcel Dekker, Inc.

Capanzana, M. V., & Buckle, K. A. (1997). Optimization of germination conditions by response surface methodology of a high amylase rice (Oryza sativa) Cultivar. LWT- Food Science and Technology, 30(2), 155-163. DOI: 10.1006/fstl.1996.0142

Chavana, P. R., Kadamb, S. S., & Beuchat, L. R. (1989). Nutritional improvement of cereals by sprouting. Critical Reviews in Food Science and Nutrition, 28(5), 401-437. DOI:10.1080/10408398909527508

Codoner-Franch, P., Hernandez-Aguilar, M. T., Navarro-Ruiz, A., Lopez-Jaem, A. B., Borja-Herrero, C., & Valls-Belles, V. (2013). Diet supplementation during early lactation with non-alcoholic beer increases the antioxidant properties of breastmilk and decreases the oxidative damage in breastfeeding mothers. Breastfeeding Medicine, 8(2), 147-148. doi: 10.1089/bfm.2012.0059

De Clerck, J. A. (1957). A Textbook of Brewing. Volume 1, translated by K. Barton-Wright. London, England: Chapman and Hall.

European Brewery Convention (EBC Analysis Committee). (1987). European Brewery Convention. Analytica-EBC. (4th ed.). Zurich, Switzerland: Brauerei-und Getränke-Rundschau.

European Herbal Infusions Association, EHIA. (2013). European Herbal Infusions Association (13 pages, 1-13). Inventory list of herbals considered as food.

Evans, D. E., Collins, H., Eglinton, J., & Wilhelmson, A. (2005). Assessing the impact of the level of diastatic power enzymes and their thermostability on the hydrolysis of starch during wort production to predict malt fermentability. Journal of the American Society of Brewing Chemists, 63(4), 185-198. DOI: 10.1094/asbcj-63-0185

German Beer Institute. (2013). Alkoholfreies beer. Retrieved August 1, 2013, from http://www.germanbeerinstitute.com/alkoholfrei.html

Goode, D. L., Wijngaard, H. H. & Arendt, E. K. (2005). Mashing with unmalted barley-impact of malted barley and commercial enzyme (Bacillus spp.) additions. Master Brewers Association of the Americas Technical Quarterly, 42(3), 184-198.

Igyor, A. M., Ogbonna, A. C., & Palmer, H. G. (2001). Effect of malting temperature and mashing methods on sorghum wort composition and beer flavour. Process Biochemistry, 36(11), 1039-1044. DOI: 10.1016/S0032-9592(00)00267-3

Kiadbanphai, D., Chahom, H., Nalad, P., & Kitluekiat, K. (2012). Preparation and properties of syrup and porridge from Thai rice malt (Special Project, Faculty of Biotechnology, Rangsit University, Patumthani, Thailand).

Kitaoka, S., & Nakano, Y. (1969). Colorimetric determination of w-amino acids. Journal of Biochemistry, 66(1), 87-94.

Komatsuzaki, N., Tsukahara, H., Toyoshima, H., Suzuki, T., Shimiz, N., & Kimura, T. (2007). Effect of soaking and gaseous treatment on GABA content in germinated brown rice. Journal of Food Engineering, 78(2), 556-560. DOI: 10.1016/j.jfoodeng.2005.10.036

Kongkaew, A., Usansa, U., & Wanapu, C. (2012). Optimisation of wort production from rice malt using enzymes and barley malt. African Journal of Biotechnology, 11(42), 9941-9949. DOI: 10.5897/AJB11.2303

Kunze, W. (2010). Technology brewing and malting. Translated by S. Pratt. (4th updated edition). Berlin, Germany: Versuchs-und Lehranstalt für Brauerei in Berlin (VLB).

Liu, S., Zhang, Z., Liu, Q., Luo, H., & Zheng, W. (2002). Spectrophotometric determination of vitamin B1 in apharmaceutical formulation using triphenylmethane acid dyes. Journal of Pharmaceutical and Biomedical Analysis, 30(3), 685-694. DOI: 10.1016/S0731-7085(02)00356-4

MacGregor, W. A., Bazin, L. S., Macri, J. L., & Babb, C. J. (1999). Modeling the contribution of alpha-amylase, beta-amylase and limit dextrinase to starch degradation during mashing. Journal of Cereal Science, 29(2), 161-169. DOI: 10.1006/jcrs.1998.0233

Martinez Alvarez, J. R., Belles, V. V., Lopez-Jaen, A.B., Marin, A. V., Codoner-Franch, P. (2009). Effects of alcohol-free beer on lipid profile and parameters of oxidative stress and inflammation in elderly women. Nutrition, 25(2), 182-187. doi:10.1016/j.nut.2008.08.005

Miller, G. L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31(3), 426-428.

Moat, A. G., & Foster, J. W. (1988). Subcellular structure of microorganism. Microbial Physiology (2nd ed.). New York, USA: John Wiley and Sons.

Moonjai, N. (2005). Comparative study of experimental beers brewed from rice malt with some starchy adjuncts. The 31st Congress on Science and Technology of Thailand. Nakhon Ratchasima Suranaree University of Technology.

Naleszkiewicz, J. (1995). Brew a great non-alcoholic beer. Brew your own: the how-to homebrew beer Magazine. Retrieved July 1, 2013, from http://byo.com/stories/item/263-brew-a-great-non-alcoholic-beer.

Ohtsubo, K., Suzuki, T. K., Yasui, Y., & Kasumi, T. (2005). Bio-functional components in the processed pre- germinated brown rice by a twin-screw extruder. Journal of Food Composition and Analysis, 18(4), 303-316. DOI: 10.1016/j.jfca.2004.10.003

Pongsawadi, P. (2002). Standard of Wort and Standard of Beer. Malt analysis manual. Khon Kaen Brewery Co. Ltd., 2-14.

Puangwerakul, Y. (2007). Malt and wort characteristics of 42 cereal rice varieties cultivated in Thailand. Kasetsart Journal: Natural Science, 41(5), 15-20. Retrieved from: http://kasetsartjnatsci.kasetsart.org/browse/index.html

Randhir, R., & Shetty, K. (2005). Developmental stimulation of total phenolics and related antioxidant activity in light- and dark-germinated corn by natural elicitors. Process Biochemistry, 40(5), 1721-1732. DOI: 10.1016/j.procbio.2004.06.064

Salubchua, J., Srakeaw, K., & Moonjai, N. (2005). Primary study of rice beer fermentation. The 31st Congress on Science and Technology of Thailand. Nakhon Ratchasima Suranaree University of Technology.

Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16(3), 144-158.

Sohrabvandi, S., Mousavi, S. M., Razavi, S. H., Mortazavian, A.M., & Rezaei, K. (2010). Alcohol-free beer: Methods of production, sensorial defects, and healthful effects. Food Reviews International, 26(4), 335-352. DOI:10.1080/87559129.2010.496022

Stewart, G. G. (2006). Adjuncts. In F. G. Priest and G. G. Stewart (Eds.), Handbook of brewing (2nd ed), (pp.161). New York, USA: Taylor & Francis.

Tanaka, K., Sugimoto, T., Ogawa, M., & Kasai, Z. (1980). Isolation and characterization of two types of protein bodies in the rice endosperm. Agricultural and Biological Chemistry, 44(7), 1633-1639.

Usansa, U. (2008). Beer production from Thai rice (Ph.D. dissertation, Suranaree University of Technology, NakornRatchasima, Thailand).

Usansa, U., Sompong, N., Wanapu, C., Boonkerd, N., & Teaumroong, N. (2009). The influences of steeping duration and temperature on the a- and b-amylase activities of six Thai rice malt cultivars (Oryza sativa L.indica). Journal of Institute of Brewing, 115(2), 140-147. DOI: 10.1002/j.2050-0416.2009.tb00359.x

Usansa, U., Burberg, F., Geiger, E., Back, W., Wanapu, C., Arendt, E. K., . . . Zarnkow, M. (2011). Optimization of malting conditions for two black rice varieties, black non-waxy rice and black waxy rice (Oryza sativa L.Indica). Journal of Institute of Brewing, 117(1), 39-46. DOI: 10.1002/j.2050-0416.2011.tb00441.x

Vinh, T. T. N., Viet, N., & Mai, T. N. (1993). The use of high percentage of rice as an adjunct in beer brewing. Master Brewers Association of the Americas Technical Quarterly, 30(2), 42-43.

Watanabe, K., Nishida, N., Adachi, T., Ueda, M., Mitsunaga, T., & Kaamura, Y. (2004). Accumulation and degradation of thiamin-binding protein and level of thiamin in wheat seeds during seed maturation and germination. Bioscience Biotechnology and Biochemistry, 68(6), 1243-1248.

Watchararpapaiboon, W., Laohakunjit, N., & Kerdchoechurn, O. (2010). An improved process for high quality and nutrition of brown rice production. Food Science Technology International, 16(2), 147-158.

Wijngaard, H. H., & Ulmer, H. M. (2005). The effect of germination temperature on malt quality of buckwheat. Journal of the American Society of Brewing Chemists, 63(1), 31-36.

Zoecklein, B. W., Fugelsang, K. C., Gump, B. H., & Nury, F. S. (1990). Production wine analysis. New York, USA: An AVI BOOK Published by Van Nostrand Reinhold.




How to Cite

Yupakanit Puangwerakul, Maneerat Lekpan, & Napaporn Khamhaeng. (2023). Effect of different mashing on non-alcoholic beer quality from Thai rice malt. Journal of Current Science and Technology, 4(2), 133–143. Retrieved from https://ph04.tci-thaijo.org/index.php/JCST/article/view/557



Research Article