Eupatorium ayapana, a natural source of anti-biofilm, anti-inflammatory and anti-oxidant agents


  • Sukanlaya Leejae College of Oriental Medicine, Rangsit University, Patumthani 12000, Thailand
  • Teeratad Sudsai College of Oriental Medicine, Rangsit University, Patumthani 12000, Thailand
  • Chat Krobthong College of Oriental Medicine, Rangsit University, Patumthani 12000, Thailand


Eupatorium ayapana, anti-biofilm formation, anti-inflammatory, anti-oxidant activity


Eupatorium ayapana is one of the most important plants used in herbal medicine.  The plant is considered to be a therapeutic agent for the treatment of various diseases.  The objective of this research was to evaluate anti-biofilm, anti-inflammatory, and anti-oxidant activities of the plant extracts.  E. ayapana leaves were extracted with hexane, CH2Cl2, and EtOAc solvents and the extracts were further utilized in biological assays.  The results demonstrated that all of the extracts exhibited pronounced anti-biofilm formation against Escherichia coli in a dose-dependent manner.  E. coli biofilm formation was inhibited more than 80% after treatment with the CH2Cl2 and EtOAc extracts (1,024 µg/ml) compared with untreated cells.  In addition, the microorganism produced biofilm 40% less after treatment with 1,024 µg/ml hexane extract.  Moreover, the established bacterial biofilm also decreased after treatment with 1,024 µg/ml of all the extracts.  For anti-oxidant activity, the EtOAc extract exhibited excellent activity against DPPH radicals with the half inhibition concentration (IC50) value at 22.7 µg/ml, which is very closed to that of BHT (24.3 µg/ml).  In addition, the CH2Cl2 and EtOAc extracts exhibited good anti-inflammatory activity against nitric oxide with IC50 values at 65.7 and 66.9 µg/ml, respectively. The differences in biological activities of the extracts of E. ayapana can be used as novel anti-biofilm, anti-inflammatory, and anti-oxidant agents.


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How to Cite

Sukanlaya Leejae, Teeratad Sudsai, & Chat Krobthong. (2023). Eupatorium ayapana, a natural source of anti-biofilm, anti-inflammatory and anti-oxidant agents. Journal of Current Science and Technology, 5(2), 141–149. Retrieved from



Research Article