Sleep phenotype of Period3 gene knockout mice under light-dark entrained condition

Thanes Fuangfoo; Watchara Damjuti; Yan-Jun Wei; Chuan-Fang Wu; Hong-Sheng Bian; Xiao-Yan Liu; Ting-Li Li

Authors

Thanes Fuangfoo Department of Pharmacology, Faculty of Pharmacy, Rangsit University, Muang, Patumthani, Thailand, 12000
Watchara Damjuti College of Public Health Sciences, Chulalongkorn University, Pathumwan, Bangkok, Thailand, 10330
Yan-Jun Wei Department of Traditional Chinese Medicine Pharmacology, College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, China, 150040
Chuan-Fang Wu Department of Traditional Chinese Medicine Pharmacology, College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, China, 150040
Hong-Sheng Bian Department of Traditional Chinese Medicine Pharmacology, College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, China, 150040
Xiao-Yan Liu Heilongjiang Vocational College, Harbin, Heilongjiang, China, 150040
Ting-Li Li Department of Traditional Chinese Medicine Pharmacology, College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, China, 150040

Keywords:

Per3 gene, circadian rhythm, sleep phenotype, sleep-wake phenotypes, NREMS, REMS

Abstract

Circadian rhythmicity contributes to sleep-wake phenotypes, and is regulated by clock genes. Although Period3 gene (Per3) is not essential for circadian rhythm generation, it does modulate circadian clock outputs and polymorphisms of this gene related to some circadian sleep disorders. We studied the role of Per3 gene on sleep-wake phenotypes by comparing Per3 knockout mice (Per3^-/-) with wild-type (WT) mice under light-dark condition. Here we report that Per3^-/- mice had more wakefulness and less non-rapid eye movement sleep (NREMS) at ZT 0 in the light period than WT mice. Likewise, in last hour of the light period on second day, wakefulness was higher and NREMS was lower in Per3 mutants. Per3^-/- mice began their main sleeping episodes later and waking episodes earlier than WT mice, whereas the average time spent in wakefulness, NREM and rapid eye movement (REM) sleep were not different between the genotypes. In addition, Per3^-/- mice had significantly less sleep bouts than WT mice at early and late light periods. These results demonstrate the influence of Per3 on sleep phenotypes related to light-dark transition and additionally show subtle effects on sleep times and sleep fragmentation.

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