Antihypertensive effect of MCHF in L-NAME induced hypertensive rats
Keywords:antihypertensive, hepatotoxicity, lipid peroxidation, Moa Chub’s formulation, MCHF, L-NAME, nitric oxide
Moa Chub’s formulation (MCHF), a traditional medicinal formulation, is used in Thailand for treatment of hypertension. It is composed of 7 kinds of medical herbs, Cyperusrotundus, Tinosporacrispa, Tinosporacordifolia, Acmellaoleracea, Syzygiumaromaticum, Piper chaba, and Zingiberofficinale. This study aimed to determine the effect of MCHF on blood pressure by investigating nitric oxide (NO) and lipid peroxidation in N-nitro-L-arginine methyl ester (L-NAME) induced hypertensive rats. Rats were gavaged with MCHF (100, 200 and 400 mg/kgBW/day) and fed with L-NAME in drinking water for 21 days. Non- invasive tail cuff was used to monitor blood pressure every 7 days. At the end of trial, blood sample was collected and plasma nitric oxide and lipid peroxidation were measured. In addition, the cytotoxicity of MCHF was evaluated in human hepatoma HepG2 cell line. Cell viability of HepG2 cells was measured by MTT assay. The results showed that administration of MCHF extract at dose of 200 and 400 mg/kgBW/day significantly decreased a rise of systolic blood pressure in hypertensive rats induced by L-NAME on the 21st day. Moreover, MCHF significantly increased the serum level of NO and reduced the level of MDA compared to the group that was treated with only L-NAME. The high doses of MCHF may lead to increased risk of hepatotoxicity. These results suggested that MCHF has the potential ability to be used as herbal remedy to treat hypertension. However, further studies are needed to explore the mechanism of antihypertensive effect of MCHF in detail.
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