Cytotoxicity and induction of the apoptotic activity of hirsutinolide series/sesquiterpene lactones from Vernonia cinerea on human colorectal cancer cells (COLO 205)


  • Suchada Jongrungruangchok College of Pharmacy, Rangsit University, Patumthani 12000, Thailand
  • Nalinee Pradubyat College of Pharmacy, Rangsit University, Patumthani 12000, Thailand
  • Thanapat Songsak College of Pharmacy, Rangsit University, Patumthani 12000, Thailand
  • Faongchat Jarintanun Faculty of Medical Technology, Rangsit University, Patumthani 12000, Thailand
  • Marisa Wall Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center, USDA-ARS, Hilo, HI, 96720, USA
  • Leng Chee Chang Department of Pharmaceutical Sciences, The Daniel K. Inouye College of Pharmacy, University of Hawaii at Hilo, Hilo 96720, USA
  • Supakit Wongwiwatthananukit Department of Pharmacy Practice, The Daniel K. Inouye College of Pharmacy, University of Hawaii at Hilo, Hilo 96720, USA


COLO 205, cytotoxicity, hirsutinolide series/sesquiterpene lactones, induction of apoptotic activity, Vernonia cinerea


Hirsutinolide series/sesquiterpene lactones from Vernonia cinerea, were investigated for cytotoxic effects and induction of the apoptotic activity on human colorectal cancer cells (COLO 205).  The results showed that hirsutinolide series significantly inhibited cell viability of COLO 205 with IC50 less than 1.5 μM.  Flow cytometric analysis indicated that hirsutinolide series (compounds 7, 10, 11, and 22) inhibited DNA synthesis as evidenced by a decline of DNA content in S, G2/M, and G0/G1 phases of the cell cycle.  Furthermore, hirsutinolide series provided anticancer activity by inducing apoptosis on COLO 205 cells through the downregulation of anti-apoptotic genes; Bcl-2 and Bcl-xL.


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How to Cite

Suchada Jongrungruangchok, Nalinee Pradubyat, Thanapat Songsak, Faongchat Jarintanun, Marisa Wall, Leng Chee Chang, & Supakit Wongwiwatthananukit. (2023). Cytotoxicity and induction of the apoptotic activity of hirsutinolide series/sesquiterpene lactones from Vernonia cinerea on human colorectal cancer cells (COLO 205). Journal of Current Science and Technology, 9(1), 41–47. Retrieved from



Research Article