Cytotoxicity and induction of the apoptotic activity of hirsutinolide series/sesquiterpene lactones from Vernonia cinerea on human colorectal cancer cells (COLO 205)

Suchada Jongrungruangchok; Nalinee Pradubyat; Thanapat Songsak; Faongchat Jarintanun; Marisa Wall; Leng Chee Chang; Supakit Wongwiwatthananukit

Authors

Suchada Jongrungruangchok College of Pharmacy, Rangsit University, Patumthani 12000, Thailand
Nalinee Pradubyat College of Pharmacy, Rangsit University, Patumthani 12000, Thailand
Thanapat Songsak College of Pharmacy, Rangsit University, Patumthani 12000, Thailand
Faongchat Jarintanun Faculty of Medical Technology, Rangsit University, Patumthani 12000, Thailand
Marisa Wall Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center, USDA-ARS, Hilo, HI, 96720, USA
Leng Chee Chang Department of Pharmaceutical Sciences, The Daniel K. Inouye College of Pharmacy, University of Hawaii at Hilo, Hilo 96720, USA
Supakit Wongwiwatthananukit Department of Pharmacy Practice, The Daniel K. Inouye College of Pharmacy, University of Hawaii at Hilo, Hilo 96720, USA

Keywords:

COLO 205, cytotoxicity, hirsutinolide series/sesquiterpene lactones, induction of apoptotic activity, Vernonia cinerea

Abstract

Hirsutinolide series/sesquiterpene lactones from Vernonia cinerea, were investigated for cytotoxic effects and induction of the apoptotic activity on human colorectal cancer cells (COLO 205). The results showed that hirsutinolide series significantly inhibited cell viability of COLO 205 with IC₅₀ less than 1.5 μM. Flow cytometric analysis indicated that hirsutinolide series (compounds 7, 10, 11, and 22) inhibited DNA synthesis as evidenced by a decline of DNA content in S, G2/M, and G0/G1 phases of the cell cycle. Furthermore, hirsutinolide series provided anticancer activity by inducing apoptosis on COLO 205 cells through the downregulation of anti-apoptotic genes; Bcl-2 and Bcl-xL.

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