Development and evaluation of “Safe, Affordable, Friendly, and Effective” UVC sterilizer for reusing N95 medical masks
Keywords:
COVID-19, ; filtering facepiece respirators (FFRs), N95 medical masks, ultraviolet germicidal irradiation (UVGI), UVC germicidal cabinet, UVC sterilizerAbstract
Shortage of NIOSH-approved N95 filtering facepiece respirators (FFRs) has made healthcare workers concerned during the COVID-19 pandemic. CDC has recommended ultraviolet germicidal irradiation (UVGI) for medical mask reuse. This study aims to develop UVC germicidal cabinet to sterilize N95 FFRs with four to five log reduction without losing protective properties. UVC germicidal cabinet is fitted with two low-pressure mercury discharge lamps (UVC T8,40W) at the top and bottom. Radiometric performance tests were conducted and exposure time for decontamination was calculated targeting 1 J×cm-2 or 10000 J×m-2. Four samples of 1870+N95 3M™ were selected to be tested after decontamination. The peak wavelength of UVC light source was 253.89 nm ± 0.60 nm with UVC irradiance value in the range from 6.56 W×m-2 at P#9 to 17.9W×m-2 at P#5. The result showed no ozone production from the lamp after one-hour monitoring with ≤1 percent instability after six minutes of lamp operation. The calculated time is 564.961 sec or 10 minutes according to maximum UVC irradiance at P#5 (17.9W×m-2), resulting in UVC dose of 3463.21 J×m-2 at minimum UVC irradiance at P#9, which still exceeds D90 values. No visible change and unfavorable odor were detected up to 12 cycles. The SEM showed no significant change up to 10 cycles, the distortion and fusion became obvious at cycle 15 and totally damaged at cycle 16, which correlated with the percentage filtration efficiency, which was lower than 95 percent, more specifically, 90.4070 percent at 16 cycle at the maximum point "P#5". This study demonstrated decontamination of N95 FFRs in dose 1 J×cm-2 up to 10 cycles without losing properties and recommended placing masks at the center of UVC germicidal cabinet to gain targeted dose. Prospective studies with additional models of N95 FFRs are required and performing strength tests on respiratory coupons and straps is recommended.
References
Chin, A., Chu, J., Perera, M., Hui, K., Yen, H.-L., Chan, M., . . . Poon, L. (2020). Stability of SARS-CoV-2 in different environmental conditions. The Lancet Microbe, 1(1), e10. DOI: 10.1016/S2666-5247(20)30003-3
Derraik, J. G. B., Anderson, W. A., Connelly, E. A., & Anderson, Y. C. (2020). Rapid evidence summary on SARS-CoV-2 survivorship and disinfection, and a reusable PPE protocol using a double-hit process. International Journal of Environmental Research and Public Health 17(17), 6117. DOI: https://doi.org/10.3390/ijerph17176117
Gurzadyan, G. G., Gorner, H., & Schulte-Frohlinde, D. (1995). Ultraviolet (193, 216 and 254 nm) photoinactivation of Escherichia coli strains with different repair deficiencies. Radiation Research, 141(3), 244-251. DOI: 10.2307/3579001
Kowalski, W. (2009). UV Surface Disinfection. In: Ultraviolet Germicidal Irradiation Handbook. Springer, Berlin, Heidelberg. DOI: https://doi.org/10.1007/978-3-642-01999-9_10
Kowalski, W., Walsh, T., & Petraitis, V. (2020). 2020 COVID-19 Coronavirus ultraviolet susceptibility. Technical Report, Report number: COVID-19_UV_V20200312, Project: UV Disinfection. https://www.researchgate.net/publication/339887436_2020_COVID-19_Coronavirus_Ultraviolet_Susceptibility
Letko, M., Marzi, A., & Munster, V. (2020). Functional assessment of cell entry and receptor usage for SARS-CoV-2 and other lineage B betacoronaviruses. Nature Microbiology, 5(4), 562-569. DOI: 10.1038/s41564-020-0688-y
Li, Q., Guan, X., Wu, P., Wang, X., Zhou, L., Tong, Y., . . . Feng, Z. (2020). Early transmission dynamics in Wuhan, China, of novel Coronavirus-infected pneumonia. New England Journal of Medicine, 382(13), 1199-1207. DOI: 10.1056/NEJMoa2001316
Liao, L., Xiao, W., Zhao, M., Yu, X., Wang, H., Wang, Q., . . . Cui, Y. (2020). Can N95 respirators be reused after disinfection? How Many Times? ACS Nano, 14(5), 6348-6356. DOI: 10.1021/acsnano.0c03597
Lindsley, W. G., Martin Jr, S. B., Thewlis, R. E., Sarkisian, K., Nwoko, J. O., Mead, K. R., & Noti, J. D. (2015). Effects of ultraviolet germicidal irradiation (UVGI) on N95 respirator filtration performance and structural integrity. Journal of occupational and environmental hygiene, 12(8), 509-517. DOI: 10.1080/15459624.2015.1018518
Lu, R., Zhao, X., Li, J., Niu, P., Yang, B., Wu, H., . . . Tan, W. (2020). Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. Lancet, 395(10224), 565-574. DOI: 10.1016/S0140-6736(20)30251-8
Matsuyama, S., Nao, N., Shirato, K., Kawase, M., Saito, S., Takayama, I., . . . Takeda, M. (2020). Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells. Proceedings of the National Academy of Sciences of the United States of America, 117(13), 7001-7003. DOI: 10.1073/pnas.2002589117
Trevisan, A., Piovesan, S., Leonardi, A., Bertocco, M., Nicolosi, P., Pelizzo, M. G., & Angelini, A. (2006). Unusual high exposure to ultraviolet-C radiation. Photochemistry and Photobiology, 82(4), 1077-1079. DOI: 10.1562/2005-10-27-ra-728
van Doremalen, N., Bushmaker, T., Morris, D., Holbrook, M., Gamble, A., Williamson, B., . . . Munster, V. (2020). Aerosol and surface stability of SARS-CoV-2 as compared with SARS-CoV-1. New England Journal of Medicine, 382. DOI: 10.1056/NEJMc2004973
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