Preparation of puerarin-loaded zein nanoparticles: characterization and stability study


  • Vilai Rungsardthong Department of Agro-Industrial, Food and Environmental Technology, Faculty of Applied Science, King Mongkut’s University of Technology North Bangkok, Bangsue, Bangkok 10800, Thailand
  • Usaraphan Pithanthanakul Department of Agro-Industrial, Food and Environmental Technology, Faculty of Applied Science, King Mongkut’s University of Technology North Bangkok, Bangsue, Bangkok 10800, Thailand
  • Chureerat Puttanlek Department of Biotechnology, Faculty of Engineering and Industrial Technology, Silpakorn University, Nakhon Pathom 73000, Thailand
  • Dudsadee Uttapap Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkhuntian, Bangkok 10150, Thailand
  • Korawinvich Boonpisuttinant Department of Thai Traditional Medicine College, Rajamangala University of Technology Thanyaburi, Prathumthani 12130, Thailand


encapsulation efficiency, Pueraria extract, puerarin, Pueraria mirifica, zein nanoparticles, zeta potential


The Thai herb Pueraria mirifica has been used in cosmetics, dietary supplements and natural health products for a long time because of its rejuvenation properties.  In this study, zein was used to enhance the stability during four-month storage of puerarin, the glycosylic isoflavone in the Pueraria extract (PE).  Zein nanoparticles (ZNs) were prepared by the liquid-liquid dispersion method.  The process was based on dissolving zein in 85% aqueous ethanol, followed by shearing the stock solution into citrate solution, and ethanol was removed by rotary vacuum evaporation.  Three different loading percentages (10, 20 and 30%) of PE were applied, and the characteristics of puerarin loaded-zein nanoparticles (PuZNs) were investigated for their size, per cent yield, per cent encapsulation efficiency (% EE) and stability.  The results showed that the particles size and zeta potential of PuZNs varied from 165.3 ± 2.4 nm to 232.8 ± 1.9 nm and -26.7 ± 0.8 mV to -31.3 ± 1.3 mV, respectively.  Increasing the % PE loading increased % yield and % EE of puerarin. Scanning electron microscopy images showed that the PuZNs were spherical with an average size of less than 200 nm.  The puerarin of Pueraria extract in the ZNs from 20 and 30% PE loading presented stability during four months of storage at 4 ± 1°C.  The results indicated the potential use of zein for encapsulation of puerarin to maintain its stability for food applications.


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How to Cite

Rungsardthong, V. ., Pithanthanakul, U. ., Puttanlek, C. ., Uttapap, D. ., & Boonpisuttinant, K. . (2023). Preparation of puerarin-loaded zein nanoparticles: characterization and stability study. Journal of Current Science and Technology, 11(1), 60–70. Retrieved from



Research Article