Growth inhibition of Escherichia coli and Pseudomonas aeruginosa strains by Piper betle Linn. extracts

Authors

  • Watcharacha Krongkeha Department of General Education, Faculty of Liberal Arts, Rajamangala University of Technology Rattanakosin, Bophit Phimuk Chakkrawat Campus, Bangkok 10100, Thailand.
  • Sineewan Pitaktim Department of Environmental Science, Faculty of Science, Udon Thani Rajabhat University, Udon Thani 41000, Thailand.

Keywords:

antibacterial activity, Escherichia coli, natural agent, Piper betle L, Pseudomonas aeruginosa

Abstract

Piper betle Linn. is a medicinal plant belonging to family Piperaceae. Its leaves are widely applied as a traditional herbal medicine due to their bioactive constituents. Ā In this study, we investigated the antibacterial activity, growth inhibition, bacterial cell morphology effect of Piper betle L. extract (PBE) against Escherichia coli ATCC 25922 and Pseudomonas aeruginosa DMST 37166. Ā PBE at the concentration of 4 mg/ml possessed the widest inhibition zones of 18.50 mm and 22.67 mm against E. coli ATCC 25922 and P. aeruginosa DMST 37166, respectively. Ā PBE showed the same minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 1 and 2 mg/ml against of E. coli ATCC 25922 and P. aeruginosa DMST 37166, respectively. Ā The killing kinetics of PBE against bacterial strains were time and dose dependent. Ā Scanning electron microscopy micrographs exhibited morphological alterations of both PBE-treated bacterial strains. Ā These results clearly indicate that the crude extract of Piper betle L. has a promising potential to be used as a natural agent for the treatment of infectious diseases caused by E. coli and P. aeruginosa.

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Published

2022-01-25

How to Cite

Krongkeha, W. ., & Pitaktim, S. . (2022). Growth inhibition of Escherichia coli and Pseudomonas aeruginosa strains by Piper betle Linn. extracts . Journal of Current Science and Technology, 12(1), 141ā€“150. retrieved from https://ph04.tci-thaijo.org/index.php/JCST/article/view/336

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Vol. 12 No. 1 (2022): January - April

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Research Article

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